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Hsp60 (HSPD1) (NM_002156) Human Over-expression Lysate

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SKU
LC400783
Lyophilized HSPD1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224428
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002156, NP_002147
Synonyms CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Predicted MW 60.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002147
Locus ID 3329
Cytogenetics 2q33.1
Protein Families WB
Protein Pathways RNA degradation, Type I diabetes mellitus
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file-alt Citations

Heat shock proteins stimulate APOBEC-3-mediated cytidine deamination in the hepatitis B virus
Chen, Eggerman, Bocharov et al
J. Biol. Chem. (2017)
AI Summary: OriGene contributed to this study by providing the A3B long form variant, which was used to investigate the role of APOBEC3 enzymes in heat shock protein-stimulated cytidine deamination of hepatitis B virus DNA.
Snippet: The A3B long form variant was purchased from Origene
Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 α1 expression
Barone, Macaluso, Sangiorgi et al
Sci Rep (2016) 6, 19781 DOI: 10.1038/srep19781
Cited by 86
AI Summary: OriGene's pCMV6-Entry-HSPD1 plasmid was used to overexpress Hsp60 in cultured myoblasts, enabling the study to demonstrate that increased Hsp60 levels induce PGC1α1 expression in skeletal muscle.
Snippet: Three point five (3.5) μg plasmid DNA [pCMV6-Entry-HSPD1] for overexpression of Hsp60 (OriGene Technologies Inc., Rockville, MD, USA) and pcDNA 3.1 plasmid for its negative control was diluted in 150 μl of Opti-MEM Medium (without FBS, L-glutamine, penicillin ... Cite SaveSaving
FigureFigureFigure
Showing 1-2 of 2 papers.
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