Rabbit IgG (Fc Fragment) Rabbit Protein

SKU RA108

Rabbit IgG Fc Fragment rabbit protein, 1 mg

Product Data

• Species: Rabbit
• Protein Source: Serum
• Concentration: lot specific
• Purity: This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
• Conjugation: Biotin
• Buffer: State: Lyophilized purified Ig fraction.; Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA; IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG F(c) molecule; Presentation Label: Biotin
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purified Ig fraction.
• Recommended Dilution: Suitable for use in Immunoblotting, ELISA, immunohistochemistry, immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit IgG, anti-Rabbit IgG F(c) and anti-Rabbit Serum.; No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain.
• Protein Description: Biotin Conjugated Rabbit IgG F(c) fragment
• Storage: Store (undiluted) at 2-8°C prior to restoration.; For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.; Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.; Centrifuge product if not completely clear after standing at room temperature.; Dilute only prior to immediate use.
• Shipping: Blue Ice

Reference Data

• Synonyms: Rabbit Immunoglobulin G
• Protein Families: anti-Rabbit IgG, anti-Rabbit IgG F(c) and anti-Rabbit Serum.<br />No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain., ELISA, immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates.<br />Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, immunohistochemistry, Suitable for use in Immunoblotting