Sm (Smith Antigen) Bovine Protein

CAT#: AR00133PU-N

Sm (Smith Antigen) bovine protein, 1 kU

Specifications

Product Data

• Species: Bovine
• Protein Source: Spleen
• Concentration: lot specific
• Purity: Immobilized human anti-Sm immune globulins. Purification is verified by SDS PAGE. Other antigens (SSA, SSB, RNP, Scl-70, Jo-1) are not detected with ELISA assays.
• Presentation: Aff - Purified
• Buffer: Presentation State: Aff - Purified
  State: Liquid purified
  Buffer System: 0.01M Tris, 0.53M Sodium chloride containing 0.02% Sodium azide
• Bioactivity: Biological: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions/protocols.
  Specific: 1000 Units/ml
• Preparation: Liquid purified
• Applications: Use in standard solid phase immunoassays.
• Protein Description: Sm (Smith) Antigen
• Storage: Store the protein at -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE. This antibody recognizes cross-reactive epitopes on the B'/B and D polypeptides of Sm. It precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes.