ATG9A (NM_024085) Human Mass Spec Standard

SKU
PH322513
ATG9A MS Standard C13 and N15-labeled recombinant protein (NP_076990)
  $3,360.00
3 Weeks*
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Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC222513
Predicted MW 94.3 kDa
Protein Sequence
Protein Sequence
>RC222513 representing NM_024085
Red=Cloning site Green=Tags(s)

MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEIFEL
MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG
VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH
RILRFQNYMVALVNKSLLPLRFRLPGLGEAVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL
ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL
QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV
CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLIL
IFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSAGQTEASVYQQAEDGKTELSLMHF
AITNPGWQPPRESTAFLGFLKEQVQRDGAAASLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSC
RGPPLPRDLQGSRHRAEVASALRSFSPLQPGQAPTGRAHSTMTGSGVDARTASSGSSVWEGQLQSLVLSE
YASTEMSLHALYMHQLHKQQAQAEPERHVWHRRESDESGESAPDEGGEGARAPQSIPRSASYPCAAPRPG
APETTALHGGFQRRYGGITDPGTVPRVPSHFSRLPLGGWAEDGQSASRHPEPVPEEGSEDELPPQVHKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_076990
RefSeq Size 3816
RefSeq ORF 2517
Synonyms APG9L1; mATG9; MGD3208
Locus ID 79065
UniProt ID Q7Z3C6
Cytogenetics 2q35
Summary Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.UniProtKB/Swiss-Prot Function
Protein Families Transmembrane
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Documents
Resources
Other "ATG9A" proteins (7)
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LC411357 Lyophilized ATG9A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC421380 Lyophilized ATG9A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC425839 Lyophilized ATG9A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY411357 Transient overexpression lysate of ATG9 autophagy related 9 homolog A (S. cerevisiae) (ATG9A), transcript variant 2 (as WB positive control) 100 ug
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LY421380 Transient overexpression lysate of ATG9 autophagy related 9 homolog A (S. cerevisiae) (ATG9A), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY425839 Transient overexpression lysate of ATG9 autophagy related 9 homolog A (S. cerevisiae) (ATG9A), transcript variant 1 (as WB positive control) 100 ug
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TP322513 Recombinant protein of human ATG9 autophagy related 9 homolog A (S. cerevisiae) (ATG9A), transcript variant 2, 20 µg 20 ug
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file-alt Citations

SLC33A1/AT-1 protein regulates the induction of autophagy downstream of IRE1/XBP1 pathway
Pehar, Jonas, Hare et al
J. Biol. Chem. (2012) 287 (35), 29921-30 DOI: 10.1074/jbc.M112.363911
Cited by 5
AI Summary: OriGene’s human AT-1 (NM_004733) cDNA (catalog #SC117182) was used in this study to investigate the role of SLC33A1/AT-1 in autophagy regulation downstream of the IRE1/XBP1 pathway.
Snippet: Plasmid Constructs. Human AT-1 (NM_004733) cDNA was obtained from OriGene (SC117182) and cloned into pcDNA3.1AMyc/His (Invitrogen) and pcDNA3.1V5/His/ TOPO (Invitrogen) (14). The cDNA of human Atg9A (NM_024085
Showing 1-1 of 1 papers.
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