DCUN1D2 (NM_001014283) Human Mass Spec Standard

CAT#: PH308865

DCUN1D2 MS Standard C13 and N15-labeled recombinant protein (NP_001014305)

Product Images

Coomassie blue staining of purified DCUN1D2 protein (Cat# TP308865). The protein was produced from HEK293T cells transfected with DCUN1D2 cDNA clone (Cat# RC208865) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC208865
• Predicted MW: 30.2 kDa
• Protein Sequence:
  Sequence Link

  >RC208865 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHKLKSSQKDKVRQFMACTQAGERTAIYCLTQNEWRLDEATDSFFQNPDSLHRESMRNAVDKKKLERLYG
  RYKDPQDENKIGVDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSRKEFLDGMTELGCDSMEKLKAL
  LPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPR
  DTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEYARPVVTGGKRSLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001014305
• RefSeq Size: 3046
• RefSeq ORF: 777
• Synonyms: C13orf17
• Locus ID: 55208
• UniProt ID: Q6PH85
• Cytogenetics: 13q34
• Summary: Potently stimulates the neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes from the NEDD8-conjugating E2 enzyme UBC12. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.[UniProtKB/Swiss-Prot Function]