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DCUN1D2 (NM_001014283) Human Tagged ORF Clone

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SKU
RC208865
DCUN1D2 (Myc-DDK-tagged)-Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DCUN1D2
Synonyms C13orf17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208865 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATAAGCTTAAATCGTCTCAGAAGGACAAGGTCCGCCAGTTTATGGCGTGCACTCAGGCTGGCGAGA
GAACTGCTATCTACTGCTTAACGCAGAATGAGTGGAGACTAGACGAGGCCACGGACAGCTTCTTCCAAAA
CCCAGACTCGCTCCACAGGGAGTCCATGCGGAACGCTGTGGACAAGAAGAAGCTGGAGCGGCTGTACGGC
AGGTACAAAGATCCACAAGATGAAAACAAAATTGGAGTCGATGGGATTCAACAGTTTTGTGATGATCTGA
GCCTGGATCCTGCCAGTATCAGTGTATTGGTCATAGCGTGGAAGTTCAGGGCAGCAACTCAGTGTGAATT
TAGCAGAAAGGAATTTCTAGATGGCATGACAGAACTTGGGTGTGACAGCATGGAGAAGCTAAAGGCTCTT
CTGCCAAGACTGGAGCAGGAGCTGAAGGACACAGCCAAGTTTAAAGATTTTTATCAGTTTACCTTCACCT
TCGCTAAGAACCCAGGGCAGAAAGGTTTAGACTTAGAAATGGCTGTTGCGTATTGGAAATTAGTGTTATC
TGGAAGGTTTAAATTTTTAGATCTCTGGAACACATTCTTAATGGAACATCACAAAAGATCAATTCCAAGG
GACACCTGGAACCTCCTGCTGGACTTTGGAAACATGATTGCGGATGATATGTCTAACTACGATGAAGAAG
GAGCTTGGCCCGTTCTTATAGATGATTTTGTAGAATATGCACGGCCAGTAGTCACAGGTGGAAAACGCAG
CCTTTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208865 protein sequence
Red=Cloning site Green=Tags(s)

MHKLKSSQKDKVRQFMACTQAGERTAIYCLTQNEWRLDEATDSFFQNPDSLHRESMRNAVDKKKLERLYG
RYKDPQDENKIGVDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSRKEFLDGMTELGCDSMEKLKAL
LPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPR
DTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEYARPVVTGGKRSLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001014283
ORF Size 777 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001014283.2
RefSeq Size 3046 bp
RefSeq ORF 780 bp
Locus ID 55208
UniProt ID Q6PH85
Cytogenetics 13q34
MW 30.2 kDa
Summary Potently stimulates the neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes from the NEDD8-conjugating E2 enzyme UBC12. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.UniProtKB/Swiss-Prot Function
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"NM_001014283" in other vectors (6)
SKU Description Size Price
RC208865L1 Lenti ORF clone of Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2), Myc-DDK-tagged 10 ug
$600.00
RC208865L2 Lenti ORF clone of Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2), mGFP tagged 10 ug
$600.00
RC208865L3 Lenti ORF clone of Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2), Myc-DDK-tagged 10 ug
$600.00
RC208865L4 Lenti ORF clone of Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2), mGFP tagged 10 ug
$600.00
RG208865 DCUN1D2 (tGFP-tagged) - Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2) 10 ug
$500.00
SC321634 DCUN1D2 (untagged)-Human DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (DCUN1D2) 10 ug
$300.00

Plasmid Map for RC208865

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208865
Circular map for RC208865

file-alt Citations

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