Knockdown Lysates

Western blotting analysis. TARDBP protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61519, 1:5,000) against TARDBP and  Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. FN1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#62510, 1:5,000) against FN1 and  Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. DDB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DDB2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ATG16L1 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ATG16L1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. CTPS1 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against CTPS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. FOXO4 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against FOXO4 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. HSF1 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSF1 and  β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. HSP90AB1 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSP90AB1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. HSPA8 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61475, 1:5,000) against HSPA8 and  β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. IQGAP2 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61138, 1:5,000) against IQGAP2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ATG7 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ATG7 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. CUL1 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against CUL1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. HSPA5 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPA5 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. HSPB8 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against HSPB8 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DES protein expression in wild-type (WT) and shRNA knockdown (KD) C2C12 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DES and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AAMP protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies against AAMP and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ABCD1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ABCD1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ABHD5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ABHD5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACAA2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61563, 1:5,000) against ACAA2 and Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ACADVL protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ACADVL and  Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACAP2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies against ACAP2 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACAT1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies (Cat#69117, 1:5,000) against ACAT1 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ACAT2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ACAT2 and  Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACBD3 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ACBD3 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACLY protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#68149, 1:5,000) against ACLY and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ACSL4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ACSL4 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACTL6A protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ACTL6A and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ACTR3 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. HSP90-Alpha served as a loading control. The blots were incubated with primary antibodies (Cat#69173, 1:5,000) against ACTR3 and HSP90-Alpha, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ADIPOR1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α α served as a loading control. The blots were incubated with primary antibodies (Cat#61573, 1:5,000) against ADIPOR1 and Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ADSS2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies (Cat#61272, 1:5,000) against ADSS2 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. AGL protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AGL and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AGO2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AGO2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AHR protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AHR and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AK4 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AK4 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AKT1S1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against AKT1S1 and  Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ALCAM protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ALCAM and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ANPEP protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against ANPEP and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. ANXA10 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61578, 1:5,000) against ANXA10 and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ANXA5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies (Cat#69585, 1:10,000) against ANXA5 and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. ANXA7 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ANXA7 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AP2M1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61579, 1:5,000) against AP2M1 and Hsp90 α , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. DSP protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DSP and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DNM1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DNM1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DPP3 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DPP3 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DPYD protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DPYD and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DNAJC12 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DNAJC12 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DSC1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DSC1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. AP2S1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  β-Tubulin α served as a loading control. The blots were incubated with primary antibodies (Cat#61329, 1:5,000) against AP2S1 and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis. DIAPH1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DIAPH1 and  Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Western blotting analysis. DLAT protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. GAPDH served as a loading control. The blots were incubated with primary antibodies against DLAT and GAPDH, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.