ALK CytoSection

CAT#: TS422485P5

Transient overexpression of ALK in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Product Images

H&E staining of ALK overexpressed cytosection (TS422485), HEK293T cells were transfected with cDNA clone RC222485, 4um sections, 10x magnification

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  H&E staining of ALK overexpressed cytosection (TS422485), HEK293T cells were transfected with cDNA clone RC222485, 4um sections, 40x magnification

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  Immunohistology staining on ALK overexpressed cytosection (TS422485) with mouse anti DDK clone 11C3 C/N TA180144 at 1:1000 dilution 4C O/N. Antibody staining was achieved with HIER Tris/EDTA pH9 for 20minutes, Polink2 detection system with DAB chromogen (D22-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC222485, 4um sections, 40x magnification

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  Immunohistology staining on HEK293T untransfected control section screened with mouse anti-DDK clone 11C3 (TA180144) at 1:1000 dilution 4C O/N. Antibody staining was achieved with HIER Tris/EDTA pH9 for 20minutes, Polink2 detection system with DAB chromogen (D22-18). No brown chromogen showing negative stain.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC222485
• Tag: C-MYC/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Target Detection Antibodies: ALK Mouse Monoclonal Antibody [Clone ID: OTI1A4] (TA801287)
• Accession Number: NM_004304, NP_004295
• Other Names: CD246; NBLST3
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• RefSeq: NP_004295
• Locus ID: 238
• Cytogenetics: 2p23.2-p23.1
• Protein Families: Druggable Genome, Protein Kinase