CYP24A1 Human qPCR Primer Pair (NM_000782)

Snippet: … Although the vitamin D receptor (VDR), a ligand dependent transcription factor, is required for growth regulation by vitamin D, the specific target genes that trigger these effects are unknown. Genomic profiling of murine mammary tumor cells with differential VDR expression identified 35 transcripts that were altered by the 1,25D3-VDR complex including Hyaluronan Synthase-2 (Has2). Here we confirmed that 1,25D3 reduces both HAS2 gene expression and hyaluronic acid (HA) synthesis in multiple models of breast cancer. … HAS2 expression and HA production are elevated in immortalized human mammary epithelial cells induced to undergo epithelial-mesenchymal transition (EMT) through stable expression of TGFβ, SNAIL or TWIST and in those expressing oncogenic H-RASV12, indicating that deregulation of HA production may be an early and frequent event in breast tumorigenesis. 1,25D3 also reduces HA secretion and acts additively with an HA synthesis inhibitor to slow growth of cells expressing TGFβ, SNAIL and TWIST. Analysis of mammary gland and tumors from Vdr knockout mice suggest that loss of VDR is associated with enhanced HAS2 expression and HA production in vivo. These data define a novel role for 1,25D3 and the VDR in control of HA synthesis in epithelial tissues that likely contributes to its anti-cancer actions

Snippet: A negative-control shRNA was used as a control. Cells were also transfected with CYP24A1-specific small-interfering RNAs (siRNAs;Santa Cruz Biotechnology, Santa Cruz, CA, USA), or a plasmid containing the full-length CYP24A1 cDNA (OriGene, Rockville, MD, ... Cite SaveSaving

Snippet: Sequence Detection System (Applied Biosystems, Foster City, CA). Primer sequences were obtained from Origene and are listed in Supplemental Table 2. Data were calculated by the ΔΔCt method and normalized against 18S expression. ... Cite SaveSaving