Albumin (ALB) Human qPCR Template Standard (NM_000477)

Snippet: For immunohistochemistry/-cytochemistry, sections or cell cultures were postfixed with 4% paraformaldehyde for 10 min at RT, washed with 10 mM PBS, blocked for 1 h at RT with 1 × PBS containing 5% bovine serum albumin (BSA), 1% normal goat serum and 0.3% Triton X-100 (Sigma), and incubated with primary antibody at 4 °C overnight. … Sections were stained with the primary antibodies to the following: FXII (1:50, Proteintech), human CD11c (1:100, clone 3.9, Abcam, Cambridge, UK), human albumin (1:50, Origene Technologies, Rockville, MD, USA), ionized calcium-binding adapter molecule 1 (1:250, polyclonal, Wako Chemicals GmbH, Neuss, Germany), glial fibrillary acidic protein (GFAP;1:1,000, clone G-A-5, Sigma-Aldrich) and mouse CD11c (1:1,000, clone N418, eBioscience, San Diego, CA, USA). …