p16INK4A (CDKN2A) Human qPCR Template Standard (BC015960)

SKU
HK200097
qPCR primer pairs and template standards against Homo sapiens gene CDKN2A
  $410.00
5 Days*
Specifications
Specifications
Product Data
Recommended Application Plasmid of exact quantity for transcript copy number calculation
ACCN BC015960
UniProt ID P42771
Synonyms ARF; CDK4 inhibitor p16-INK4; CDK4I; CDKN2; cell cycle negative regulator beta; CMM2; cyclin-dependent ki; cyclin-dependent kinase inhibitor 2A; INK4; INK4a; MLM; MTS1; OTTHUMP00000021147; OTTHUMP00000021148; p14; p14ARF; p16; p16INK4; p16INK4a; p19; TP16
Components 1 vial of lyophilized SybGREEN qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)
1 vial lyophilized Gene Specific qPCR template standard: 50 X 10^7 copies, double-stranded DNA
1 vial containing 1 mL 10x PCR template standard dilution buffer
Quality Control The kit has been tested to generate satisfactory qPCR data on ABI 7900HT
Storage Store at -20°C.
Stability The primer mix and template standard are stable for one year from date of shipping.
Shipping Ambient
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Documents
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file-alt Citations

The dual distinct role of telomerase in repression of senescence and myofibroblast differentiation
Harada, Hu, Lu et al
Aging (Albany NY) (2021) 13 (13), 16957-16973 DOI: 10.18632/aging.203246
Cited by 9
AI Summary: OriGene’s pCMV-mycDDK-CDKN2A plasmid was used to transfect cells for studying the effects of CDKN2A overexpression on senescence and myofibroblast differentiation in this research.
Snippet: the cells were transfected with human CDKN2A plasmid with the DDK tag (pCMV- mycDDK- CDKN2A plasmid, OriGENE, Rockville, MD)
FigureFigureFigure
Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation
Xiao, He, Liu et al
Oncol. Rep. (2019)
AI Summary: OriGene’s P16INK4A antibody (cat. no. [not specified in snippet]) and secondary antibody (cat. no. SP-9001) were used in this study to detect and analyze P16INK4A protein levels, facilitating the investigation of its role in uterine serous carcinoma.
Snippet: The membranes were blocked with 5% milk at room temperature for 1 h and the target proteins were probed by the following antibodies for 12 h at 4˚C: P16INK4A (OriGene Technologies, Inc., Rockville, MD, USA;cat. no no. SP-9001;OriGene Technologies, Inc.)
WB
Raltitrexed Inhibits HepG2 Cell Proliferation via G0/G1 Cell Cycle Arrest
Zhao, Zhang, Sun et al
Oncol. Res. (2016) 23 (5), 237-48 DOI: 10.3727/096504016X14562725373671
AI Summary: In this study, OriGene products contributed by providing antibodies against TS, CDK2, p53, and p16, which were used in Western blot analyses to assess the expression of key cell cycle regulatory proteins in HepG2 cells treated with raltitrexed.
Snippet: Bradford assay kit was purchased from Jiancheng Bioengineering (Nanjing, China). Antibodies against TS, CDK2, p53, and p16 were from Origene (Rockville, MD, USA). Anti-cyclin A was purchased from Bioworld Technology (Atlanta, GA, USA). ... Cite SaveSaving
WB
Effect of tumor suppressor gene cyclin-dependent kinase inhibitor 2A wild-type and A148T mutant on the cell cycle of human ovarian cancer cells
Bian, Yu, Yang et al
Oncol Lett (2014) 7 (4), 1229-1232 DOI: 10.3892/ol.2014.1867
Cited by 10
AI Summary: OriGene’s pTUNE inducible vector system and CDKN2A/p16 wild-type and A148T mutant open reading frame products were used to construct expression systems that enabled the authors to investigate the effects of a single-base mutation in CDKN2A/p16 on cell cycle arrest in ovarian cancer cells.
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Showing 1-4 of 4 papers.
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