BCL2 Human qPCR Template Standard (BC027258)

Snippet: the cells were co-transfected with miR-185-5p (Qia- gen, USA) and luciferase reporter plasmid containing 3′UTR of BCL2 (OriGene, USA) and transfected with luciferase reporter plasmid as a control group by using Lipofectamine 2000 (Thermo Fisher Scientific, USA;BT-474 cells (3 × 104 cells/well) were seeded into a 96-well plate and cultured for 24 h. Subsequently, the cells were co-transfected with miR-185-5p (Qiagen, USA) and luciferase reporter plasmid containing 3′UTR of BCL2 (OriGene, USA) and transfected with luciferase reporter plasmid as a control group by using Lipofectamine 2000 (Thermo Fisher Scientific, USA) according to manufacturer’s protocol. …

Snippet: Then, membranes were washed by TBST 3 times and incubated overnight at 4°C with primary antibodies against FAM111B (PA5-58474;Invitrogen), BAG3 (AB47124;Abcam), BCL2 (TA806639S;Origene), β-actin (8H10D10;Cell Signaling Technology) at a 1:1,000

Snippet: Using DICER1 siRNA, we next substantially knocked down the DICER1 expression (Figure 5a) in wild-type ΔNp63α cells ... USA), whereas 3′-UTR luciferase reporter plasmids for HIPK1 (SC221730) and BCL2 (SC222289) were purchased from Origene Technologies (Rockville