Tnf Mouse qPCR Primer Pair (NM_013693)

Snippet: hesis was performed using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, United States). For qPCR, the resulting cDNA was mixed with iQ SYBR Green Supermix (Bio-Rad) and primer set for TNF-α (Origene, Rockville, MD, United States, cat. #: MP217748), IL-6 (Origene, cat. #: MP206798), IL-10 (Qiagen, cat. #: PPM03017C-200), IFN-γ (Origene, cat. #: MP206683), IL-17 (Origene, cat. #: MP206759)

Snippet: For this, forward and reverse primers for IFN-γ, IL-12, and IL-10 were designed following the sequence mentioned in Table 1. Likewise, the primer sequence for the TNF-α cytokines was taken from the OriGene website (Rockville, MD, USA)

Snippet: Ct value for the expression of these cytokines was normalized with ACTB and relative fold change was calculated with respect to mean of healthy controls. The primer sequence for the mentioned cytokines were taken from OriGene website. Page 6. Accepted Article

Snippet: Primers for IL-17 421 (MP206759), IL-1β (MP206724), IL-6 (MP206798), CXCL-1 (MP202409), TNF422 α (MP217748), CXCL-2 (MP202418), MMP-9 (MP207904), and G-CSF (MP202847) were 423 purchased from OriGene. All the data were normalized to the GAPDH expression level in the 424 same sample, and mRNA levels were calculated by the ΔΔCt method as compared with the 425 controls