SPAG17 Human qPCR Primer Pair (NM_206996)

SKU
HP219614
qSTAR qPCR primer pairs against Homo sapiens gene SPAG17
  $150.00
5 Days*
Specifications
Specifications
Product Data
Locus ID 200162
Forward Sequence CCAGAGCCTAAATGTGTCTTGCC
Reverse Sequence GGTAGCTCTGACGGACCATGAT
ACCN NM_206996, NM_206996.2, NM_206996.3, BC043251, BC148427, BX648110, NM_206996.4
UniProt ID Q6Q759
Synonyms CT143; PF6; SPGF55
Components 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
Quality Control The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec.
Storage Store at -20°C.
Stability The primer mix is stable for one year from date of shipping.
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Documents
Resources

file-alt Citations

Spag17 deficiency results in skeletal malformations and bone abnormalities
Teves, Sundaresan, Cohen et al
PLoS One (2015) 10 (5), e0125936 DOI: 10.1371/journal.pone.0125936
Cited by 40
AI Summary: OriGene’s Spag17 siRNA duplex (SR421841) was used to knock down Spag17 expression in wild-type mouse embryonic fibroblasts, helping to demonstrate that reduced Spag17 levels lead to shorter primary cilia, thus implicating Spag17 in skeletal growth and bone abnormalities.
FigureFigureFigure
Showing 1-1 of 1 papers.
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