PI 3 Kinase catalytic subunit alpha (PIK3CA) (NM_006218) Human Mutant ORF Clone
- TrueORF®
SKU
RC400348
PIK3CA Mutant (E545K), Myc-DDK-tagged ORF clone of Homo sapiens phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA) as transfection-ready DNA
$930.00
In Stock*
Specifications
| Product Data | |
| Mutation Description | E545K |
|---|---|
| Affected Codon# | 545 |
| Affected NT# | c.1633 |
| Tag | Myc-DDK |
| Effect | Missense |
| Target Symbol | PI 3 Kinase catalytic subunit alpha |
| Synonyms | CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; PI3K; PI3K-alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_006218 |
| ORF Size | 3204 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_006209 |
| RefSeq Size | 3724 bp |
| RefSeq ORF | 3207 bp |
| Locus ID | 5290 |
| UniProt ID | P42336 |
| Cytogenetics | 3q26.32 |
| Domains | PI3Ka, PI3K_C2, PI3K_p85B, PI3K_rbd, PI3_PI4_kinase |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway |
| MW | 124 kDa |
| Summary | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. provided by RefSeq, Apr 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-altered prostate cancers
Mao, Zhang, Lee et al
Nat Commun (2021) 12 (1), 5053 DOI: 10.1038/s41467-021-25341-9
Mao, Zhang, Lee et al
Nat Commun (2021) 12 (1), 5053 DOI: 10.1038/s41467-021-25341-9
Cited by 31
AI Summary: OriGene’s pLenti-Lentiviral vector (pLenti-C-Myc-DDK-P2A-Neo, PS100081) was used to achieve stable gene expression in cell models, enabling the authors to investigate PI3K pathway dependencies in prostate cancer subtypes.
Snippet: the reaction. All cell proliferation assays were repeated in at least two independent biological replicates. Stable gene expression analysis. pLenti-Lentiviral vector (pLenti-C-Myc-DDK-P2A-Neo) (Origene, PS100081) was used
AKT1 E17K Inhibits Cancer Cell Migration by Abrogating β-Catenin Signaling
Gao, Kiliti, Zhang et al
Mol Cancer Res (2020)
Gao, Kiliti, Zhang et al
Mol Cancer Res (2020)
AI Summary: In this study, OriGene's PIK3CA E545K mutant cDNA (Cat# RC400348) and Lenti-C-Myc-DDK-IRES-Neo plasmid (Cat# PS100081) were used to generate a PIK3CA-E545K lentiviral expression vector for functional analyses of cancer cell migration.
Snippet: … PIK3CA E545K mutant cDNA (Cat#RC400348), purchased from OriGene, was inserted into the AsiS I and Mlu I sites of plasmid Lenti-C-Myc-DDK- IRES-Neo (Cat#PS100081, OriGene), to generate PIK3CA-E545K lentiviral expression vector;- cloning purpose. PIK3CA E545K mutant cDNA (Cat#RC400348), purchased from OriGene, was inserted into the AsiS I and Mlu I sites of plasmid Lenti-C-Myc-DDK- IRES-Neo (Cat#PS100081, OriGene), to generate PIK3CA-E545K lentiviral expression vector
Atorvastatin overcomes gefitinib resistance in KRAS mutant human non-small cell lung carcinoma cells
Chen, Bi, Hou et al
Cell Death Dis (2013) 4, e814 DOI: 10.1038/cddis.2013.312
Chen, Bi, Hou et al
Cell Death Dis (2013) 4, e814 DOI: 10.1038/cddis.2013.312
Cited by 66
AI Summary: OriGene's Human TrueORF PIK3CA E545K plasmid (Cat. no. RC400348) was used in this study to generate NSCLC cell models with PIK3CA mutations, enabling the investigation of atorvastatin’s effects on overcoming gefitinib resistance in KRAS mutant cells.
Snippet: 30 Human TrueORF PIK3CA E545K plasmid was purchased from OriGene Technologies (Rockville, MD, USA;Cat. no. RC400348). TrueORFs have a C-terminal fusion of MYC/DDK tag. The cloning expression vector is pCMV6-Entry.
Showing 1-3 of 3 papers.
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