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EGFR (NM_005228) Human Mutant ORF Clone

  • TrueORF®
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SKU
RC400290
EGFR Mutant (L858R), Myc-DDK-tagged ORF clone of Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1 as transfection-ready DNA
  $1,580.00
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Specifications
Specifications
Product Data
Mutation Description L858R
Affected Codon# 858
Affected NT# c.2573
Tag Myc-DDK
Effect Missense
Target Symbol EGFR
Synonyms ERBB; ERBB1; ERRP; HER1; mENA; NISBD2; PIG61
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_005228
ORF Size 3630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NP_005219
RefSeq Size 5616 bp
RefSeq ORF 3633 bp
Locus ID 1956
UniProt ID P00533
Cytogenetics 7p11.2
Domains FU, Furin-like, pkinase, Recep_L_domain, S_TKc, TyrKc
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton
MW 134 kDa
Summary The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). provided by RefSeq, Jul 2020
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Documents
Resources

file-alt Citations

Licochalcone A inhibits EGFR signalling and translationally suppresses survivin expression in human cancer cells
Gao, Li, Yu et al
J Cell Mol Med (2020)
Cited by 31
AI Summary: OriGene contributed EGFR cDNA clones (L858R EGFR, L858R/T790M EGFR, Del E746‐A750 EGFR, and wild-type EGFR), which were used to generate stable cell lines for investigating the effects of licochalcone A on EGFR signalling in human cancer cells.
Snippet: 2.4 Lentiviral package and stable lines generation. The EGFR cDNA clones, including L858R EGFR, L858R/T790M EGFR, Del E746‐A750 EGFR and wild‐type EGFR, were obtained from Origene and used for virus package
Showing 1-1 of 1 papers.
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