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IDH2 (NM_002168) Human Mutant ORF Clone

  • TrueORF®
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SKU
RC400103
IDH2 mutant (R172K), Myc-DDK-tagged ORF clone of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2), nuclear gene encoding mitochondrial protein as transfection-ready DNA
  $457.00
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Specifications
Specifications
Product Data
Mutation Description R172K
Affected Codon# 172
Affected NT# c. 515
Tag Myc-DDK
Effect Missense
Target Symbol IDH2
Synonyms D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC400103 representing NM_002168
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCTACCTGCGGGTCGTGCGCTCGCTCTGCAGAGCCTCAGGCTCGCGGCCGGCCTGGGCGCCGG
CGGCCCTGACAGCCCCCACCTCGCAAGAGCAGCCGCGGCGCCACTATGCCGACAAAAGGATCAAGGTGGC
GAAGCCCGTGGTGGAGATGGATGGTGATGAGATGACCCGTATTATCTGGCAGTTCATCAAGGAGAAGCTC
ATCCTGCCCCACGTGGACATCCAGCTAAAGTATTTTGACCTCGGGCTCCCAAACCGTGACCAGACTGATG
ACCAGGTCACCATTGACTCTGCACTGGCCACCCAGAAGTACAGTGTGGCTGTCAAGTGTGCCACCATCAC
CCCTGATGAGGCCCGTGTGGAAGAGTTCAAGCTGAAGAAGATGTGGAAAAGTCCCAATGGAACTATCCGG
AACATCCTGGGGGGGACTGTCTTCCGGGAGCCCATCATCTGCAAAAACATCCCACGCCTAGTCCCTGGCT
GGACCAAGCCCATCACCATTGGCAAGCACGCCCATGGCGACCAGTACAAGGCCACAGACTTTGTGGCAGA
CCGGGCCGGCACTTTCAAAATGGTCTTCACCCCAAAAGATGGCAGTGGTGTCAAGGAGTGGGAAGTGTAC
AACTTCCCCGCAGGCGGCGTGGGCATGGGCATGTACAACACCGACGAGTCCATCTCAGGTTTTGCGCACA
GCTGCTTCCAGTATGCCATCCAGAAGAAATGGCCGCTGTACATGAGCACCAAGAACACCATACTGAAAGC
CTACGATGGGCGTTTCAAGGACATCTTCCAGGAGATCTTTGACAAGCACTATAAGACCGACTTCGACAAG
AATAAGATCTGGTATGAGCACCGGCTCATTGATGACATGGTGGCTCAGGTCCTCAAGTCTTCGGGTGGCT
TTGTGTGGGCCTGCAAGAACTATGACGGAGATGTGCAGTCAGACATCCTGGCCCAGGGCTTTGGCTCCCT
TGGCCTGATGACGTCCGTCCTGGTCTGCCCTGATGGGAAGACGATTGAGGCTGAGGCCGCTCATGGGACC
GTCACCCGCCACTATCGGGAGCACCAGAAGGGCCGGCCCACCAGCACCAACCCCATCGCCAGCATCTTTG
CCTGGACACGTGGCCTGGAGCACCGGGGGAAGCTGGATGGGAACCAAGACCTCATCAGGTTTGCCCAGAT
GCTGGAGAAGGTGTGCGTGGAGACGGTGGAGAGTGGAGCCATGACCAAGGACCTGGCGGGCTGCATTCAC
GGCCTCAGCAATGTGAAGCTGAACGAGCACTTCCTGAACACCACGGACTTCCTCGACACCATCAAGAGCA
ACCTGGACAGAGCCCTGGGCAGGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC400103 representing NM_002168
Red=Cloning site Green=Tags(s)

MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPVVEMDGDEMTRIIWQFIKEKL
ILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIR
NILGGTVFREPIICKNIPRLVPGWTKPITIGKHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVY
NFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDK
NKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT
VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIH
GLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_002168
ORF Size 1356 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NP_002159
RefSeq Size 1740 bp
RefSeq ORF 1359 bp
Locus ID 3418
UniProt ID P48735
Cytogenetics 15q26.1
Domains isodh
Protein Pathways Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways
MW 46.6 kDa
Summary Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2014
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Documents
Resources

file-alt Citations

Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate
Showalter, Hatakeyama, Cajka et al
Elife (2017) 6 DOI: 10.7554/eLife.26030
AI Summary: OriGene’s pCMV6-Entry vectors containing IDH2WT and IDH2R172K were used as templates to generate expression constructs for studying the mutant and wild-type IDH2 enzymes in this replication study.
Snippet: Plasmids and transfection To generate pcDNA3.1-IDH2WT (deposited in Addgene, plasmid# 87926) and pcDNA3.1-IDH2R172K (deposited in Addgene, plasmid# 87927), IDH2WT and IDH2R172K were amplified by PCR from pCMV6-Entry vectors (Origene, Rockville ... All 7 versions Cite SaveSaving
FigureFigureFigure
Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation
Richarson, Scott, Zagnitko et al
Elife (2016) 5 DOI: 10.7554/eLife.10860
AI Summary: OriGene products, specifically the IDH1 ORF clone (RC210582), IDH1 R132H ORF clone (RC400096), and IDH2 ORF clone (RC201152), were used as expression constructs to replicate key experiments investigating how mutant IDH1/2 affects histone methylation and cell differentiation.
Snippet: IDH1 ORF clone, Nucleic acid, OriGene, RC210582, Replaces ATCC plasmid in pCMV-Sport6. IDH1 R132H ORF clone, Nucleic acid, OriGene, RC400096, Original generated by authors. IDH2 ORF clone, Nucleic acid, OriGene, RC201152, Replaces Invitrogen plasmid ... Cite SaveSaving
Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate
Fiehn, Showalter, Schaner-Tooley et al
Elife (2016) 5 DOI: 10.7554/eLife.12626
AI Summary: OriGene products contributed to this study by providing the IDH2 WT ORF in pCMV6 plasmid (RC201152) and the IDH2 R172K ORF in pCMV6 plasmid (RC400103), which were used to study the effects of IDH2 mutations on enzyme activity.
Snippet: FBS, Reagent, Hyclone, SH30071.03, Replaces FBS from CellGro. IDH2 WT ORF in pCMV6, Plasmid, Origene, RC201152, IDH2 R172K ORF in pCMV6, Plasmid, Origene, RC400103, ... Sub-clone IDH2 WT and IDH2 R172K from the Origene pCMV6-Entry vectors into ... Cite SaveSaving
Showing 1-3 of 3 papers.
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