Isocitrate dehydrogenase (IDH1) (NM_005896) Human Mutant ORF Clone
- TrueORF®
SKU
RC400096
IDH1 mutant (R132H), Myc-DDK-tagged ORF clone of Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) as transfection-ready DNA
$457.00
In Stock*
Specifications
| Product Data | |
| Mutation Description | R132H |
|---|---|
| Affected Codon# | 132 |
| Affected NT# | c. 395 |
| Tag | Myc-DDK |
| Effect | Missense |
| Target Symbol | Isocitrate dehydrogenase |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_005896 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_005887 |
| RefSeq Size | 2339 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 3417 |
| UniProt ID | O75874, A0A024R3Y6 |
| Cytogenetics | 2q34 |
| Domains | isodh |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
| MW | 46.5 kDa |
| Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Sep 2013 |
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Resources
Citations
Convection-enhanced delivery of nanoencapsulated gene locoregionally yielding ErbB2/Her2-specific CAR-macrophages for brainstem glioma immunotherapy
Gao, Shi, Yang et al
J Nanobiotechnology (2023) 21 (1), 56 DOI: 10.1186/s12951-023-01810-9
Gao, Shi, Yang et al
J Nanobiotechnology (2023) 21 (1), 56 DOI: 10.1186/s12951-023-01810-9
Cited by 70
AI Summary: OriGene’s ErbB2 cDNA plasmid (RC400096) was used to generate ErbB2-expressing GL261 and Luci+GL261 cell lines, enabling the study of ErbB2-targeted CAR-macrophage immunotherapy in brainstem glioma models.
Snippet: e-conditioned medium (20 ng ml−1 IL-4). Both the murine GL261 and Luci + GL261 cell lines (gifted from Jiangsu University) were transfected with 10 μg plasmids that encoded cDNAs for ErbB2 (OriGene Technologies, RC400096) to obtain the ErbB2 + and Luci + GL261 ErbB2 cell lines (named GL261-H and Luci + GL261-H cell lines), and the Luci + GL261-H cell line was used for in
Limited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors
Vaziri-Gohar, Cassel, Mohammed et al
Nat Cancer (2022) 3 (7), 852-865 DOI: 10.1038/s43018-022-00393-y
Vaziri-Gohar, Cassel, Mohammed et al
Nat Cancer (2022) 3 (7), 852-865 DOI: 10.1038/s43018-022-00393-y
Cited by 87
AI Summary: OriGene products—wild-type IDH1 overexpression plasmid (SC116430) and mutant IDH1 (R132H) overexpression plasmid (RC400096)—were used in this study to investigate the functional roles of IDH1 variants in pancreatic cancer cell adaptation to nutrient deprivation and sensitivity to IDH1 inhibitors.
Snippet: …A9165), wild-type IDH1 overexpression (Origene, no. SC116430) and mutant IDH1 (R132H) overexpression (Origene, no. RC400096) were used. CRISPR–Cas9 editing of IDH1 in pancreatic cancer cells IDH1 knockout was performed using a guideRNA:;GSH (Sigma, no. … A9165), wild-type IDH1 overexpression (Origene, no. SC116430) and mutant IDH1 (R132H) overexpression (Origene, no. RC400096) were used
ATRX regulates glial identity and the tumor microenvironment in IDH-mutant glioma
Babikir, Wang, Shamardani et al
Genome Biol (2021) 22 (1), 311 DOI: 10.1186/s13059-021-02535-4
Babikir, Wang, Shamardani et al
Genome Biol (2021) 22 (1), 311 DOI: 10.1186/s13059-021-02535-4
Cited by 52
AI Summary: OriGene products, specifically the IDH1R132H plasmid (CAT#: RC400096) and wildtype IDH1 plasmid (CAT#: RC210582), were used to transfect SB28 ATRX-KO cells, enabling the study of IDH1 mutations in the context of ATRX loss in glioma models.
Snippet: SB28 ATRX-KO cells were transfected with plasmids expressing either IDH1R132H (ORIGENE, CAT#: RC400096) or wildtype IDH1 (Origene CAT#: RC210582) and selected via neomycin. Cells were cultured in RPMI media supplemented with 10% FBS and 100
Immunostimulant hydrogel for the inhibition of malignant glioma relapse post-resection
Zhang, Chen, Li et al
Nat Nanotechnol (2021)
Zhang, Chen, Li et al
Nat Nanotechnol (2021)
Cited by 311
AI Summary: OriGene’s R132H IDH-MUT cDNA plasmid (RC400096) was used to transfect GL261 and Luci+ GL261 cells, enabling the creation of mutant cell lines essential for evaluating the hydrogel’s immunostimulatory effects in this study.
Snippet: Murine GL261 and Luci+ GL261 cells were gifted from A.G.’s lab at Jiangsu University and then transfected with 10 μg plasmids that encoded cDNAs for R132H IDH-MUT (OriGene Technologies, RC400096) to obtain GL261R132H and Luci+ GL261R132H cells
G-CSF Secreted by Epigenetically Reprogrammed Mutant IDH1 Glioma Stem Cells Reverses the Myeloid Cells’-Mediated Immunosuppressive Tumor Microenvironment
Alghamri, Avvari, Thalla et al
bioRxiv (2020)
Alghamri, Avvari, Thalla et al
bioRxiv (2020)
Cited by 3
Snippet: … SJGBM2 cells (1.5 x 105 each per well) were seeded in a 6-well plate, and after 24 hours they were transfected with p-CMV-IDH1-R132H-Entry plasmid (Origene, RC400096) using jetPRIME transfection system (Polyplus, 114-07) according to the manufacturer’s instructions and as described before (19). … On day 15 of selection, individual cell colonies were taken from the well using autoclaved filter paper (Whatman, 1001-185) squares (2x2 mm approx.) previously embedded in HyQTase Cell Detachment Solution (GE Healthcare Life Science, HyClone, SV30030.01) and placed in wells of 24-well plates with the appropriate cell culture medium. …
Wild-Type IDH1 and Mutant IDH1 Opposingly Regulate Podoplanin Expression in Glioma
Sun, Xiao, Zhao et al
Transl Oncol (2020) 13 (4), 100758 DOI: 10.1016/j.tranon.2020.100758
Sun, Xiao, Zhao et al
Transl Oncol (2020) 13 (4), 100758 DOI: 10.1016/j.tranon.2020.100758
Cited by 25
AI Summary: OriGene's pLenti-C-Myc-DDK-IRES-Puro lentiviral vector was used to generate constructs for overexpressing full-length podoplanin (Pdpn), enabling the authors to study how wild-type and mutant IDH1 regulate Pdpn expression and glioma cell migration.
Snippet: Lentivirus Generation and Infection. Full-length Pdpn (NM_006474.4) was subcloned into the AsiSI and XhoI restriction sites of the lentiviral vector pLenti-C-Myc-DDK-IRES-Puro (Origene), and the sequence was confirmed by Sanger sequencing
Showing 1-6 of 10 papers.
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