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HPSE2 (NM_001166244) Human Untagged Clone

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SKU
SC328880
HPSE2 (untagged)-Human heparanase 2 (HPSE2) transcript variant 2
  $548.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HPSE2
Synonyms HPA2; HPR2; UFS; UFS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC328880 representing NM_001166244.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGGGTGCTTTGTGCCTTCCCTGAAGCCATGCCCTCCAGCAACTCCCGCCCCCCCGCGTGCCTAGCC
CCGGGGGCTCTCTACTTGGCTCTGTTGCTCCATCTCTCCCTTTCCTCCCAGGCTGGAGACAGGAGACCC
TTGCCTGTAGACAGAGCTGCAGGTTTGAAGGAAAAGACCCTGATTCTACTTGATGTGAGCACCAAGAAC
CCAGTCAGGACAGTCAATGAGAACTTCCTCTCTCTGCAGCTGGATCCGTCCATCATTCATGATGGCTGG
CTCGATTTCCTAAGCTCCAAGCGCTTGGTGACCCTGGCCCGGGGACTTTCGCCCGCCTTTCTGCGCTTC
GGGGGCAAAAGGACCGACTTCCTGCAGTTCCAGAACCTGAGGAACCCGGCGAAAAGCCGCGGGGGCCCG
GGCCCGGATTACTATCTCAAAAACTATGAGGATGACATTGTTCGAAGTGATGTTGCCTTAGATAAACAG
AAAGGCTGCAAGATTGCCCAGCACCCTGATGTTATGCTGGAGCTCCAAAGGGAGAAGGCAGCTCAGATG
CATCTGGTTCTTCTAAAGGAGCAATTCTCCAATACTTACAGTAATCTCATATTAACAGAGCCAAATAAC
TATCGGACCATGCATGGCCGGGCAGTAAATGGCAGCCAGTTGGGAAAGGATTACATCCAGCTGAAGAGC
CTGTTGCAGCCCATCCGGATTTATTCCAGAGCCAGCTTATATGGCCCTAATATTGGGCGGCCGAGGAAG
AATGTCATCGCCCTCCTAGATGGATTCATGAAGGTGGCAGGAAGTACAGTAGATGCAGTTACCTGGCAA
CATTGCTACATTGATGGCCGGGTGGTCAAGGTGATGGACTTCCTGAAAACTCGCCTGTTAGACACACTC
TCTGACCAGATTAGGAAAATTCAGAAAGTGGTTAATACATACACTCCAGGAAAGAAGATTTGGCTTGAA
GGTGTGGTGACCACCTCAGCTGGAGGCACAAACAATCTATCCGATTCCTATGCTGCAGGATTCTTATGG
TTGAACACTTTAGGAATGCTGGCCAATCAGGGCATTGATGTCGTGATACGGCACTCATTTTTTGACCAT
GGATACAATCACCTCGTGGACCAGAATTTTAACCCATTACCAGACTACTGGCTCTCTCTCCTCTACAAG
CGCCTGATCGGCCCCAAAGTCTTGGCTGTGCATGTGGCTGGGCTCCAGCGGAAGCCACGGCCTGGCCGA
GTGATCCGGGACAAACTAAGGATTTATGCTCACTGCACAAACCACCACAACCACAACTACGTTCGTGGG
TCCATTACACTTTTTATCATCAACTTGCATCGATCAAGAAAGAAAATCAAGCTGGCTGGGACTCTCAGA
GACAAGCTGGTTCACCAGTACCTGCTGCAGCCCTATGGGCAGGAGGGCCTAAAGTCCAAGTCAGTGCAA
CTGAATGGCCAGCCCTTAGTGATGGTGGACGACGGGACCCTCCCAGAATTGAAGCCCCGCCCCCTTCGG
GCCGGCCGGACATTGGTCATCCCTCCAGTCACCATGGGCTTTTATGTGGTCAAGAATGTCAATGCTTTG
GCCTGCCGCTACCGATAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001166244
Insert Size 1605 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166244.1
RefSeq Size 4175 bp
RefSeq ORF 1605 bp
Locus ID 60495
UniProt ID Q8WWQ2
Cytogenetics 10q24.2
Protein Pathways Glycosaminoglycan degradation, Metabolic pathways
MW 60.1 kDa
Summary This gene encodes a heparanase enzyme. The encoded protein is a endoglycosidase that degrades heparin sulfate proteoglycans located on the extracellular matrix and cell surface. This protein may be involved in biological processes involving remodeling of the extracellular matrix including angiogenesis and tumor progression. Alternate splicing results in multiple transcript variants. provided by RefSeq, Oct 2009
Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001166244" in other vectors (4)
SKU Description Size Price
RC230242 HPSE2 (Myc-DDK-tagged)-Human heparanase 2 (HPSE2), transcript variant 2 10 ug
$497.00
RC230242L3 Lenti ORF clone of Human heparanase 2 (HPSE2), transcript variant 2, Myc-DDK-tagged 10 ug
$797.00
RC230242L4 Lenti ORF clone of Human heparanase 2 (HPSE2), transcript variant 2, mGFP tagged 10 ug
$797.00
RG230242 HPSE2 (tGFP-tagged) - Human heparanase 2 (HPSE2), transcript variant 2 10 ug
$697.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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