MPP1 (NM_001166461) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | MPP1 |
| Synonyms | AAG12; DXS552E; EMP55; MRG1; PEMP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_001166461 |
| Insert Size | 1341 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001166461.1 |
| RefSeq Size | 2007 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 4354 |
| UniProt ID | Q00013 |
| Cytogenetics | Xq28 |
| Protein Families | Druggable Genome |
| MW | 49.9 kDa |
| Summary | This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2009 Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. |
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Resources
"NM_001166461" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC230095 | MPP1 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3 | 10 ug |
$457.00
|
|
| RC230095L3 | Lenti ORF clone of Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC230095L4 | Lenti ORF clone of Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3, mGFP tagged | 10 ug |
$757.00
|
|
| RG230095 | MPP1 (tGFP-tagged) - Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3 | 10 ug |
$657.00
|
|
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