MPP1 (NM_001166461) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC230095L4
Lenti ORF clone of Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3, mGFP tagged
  $757.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MPP1
Synonyms AAG12; DXS552E; EMP55; MRG1; PEMP
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC230095).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166461
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166461.1
RefSeq ORF 1341 bp
Locus ID 4354
UniProt ID Q00013, B4DZV5
Cytogenetics Xq28
Protein Families Druggable Genome
MW 50.3 kDa
Summary This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2009
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"NM_001166461" in other vectors (4)
SKU Description Size Price
RC230095 MPP1 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3 10 ug
$457.00
RC230095L3 Lenti ORF clone of Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RG230095 MPP1 (tGFP-tagged) - Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3 10 ug
$657.00
SC328733 MPP1 (untagged)-Human membrane protein palmitoylated 1 55kDa (MPP1) transcript variant 3 10 ug
$503.00

Plasmid Map for RC230095L4

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Cloning scheme for RC230095L4

Circular map for RC230095L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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