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ADAM8 (NM_001164489) Human Untagged Clone

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SKU
SC327203
ADAM8 (untagged)-Human ADAM metallopeptidase domain 8 (ADAM8) transcript variant 2
  $748.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ADAM8
Synonyms CD156; CD156a; MS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC327203 representing NM_001164489.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCGCGGCCTCGGGCTCTGGCTGCTGGGCGCGATGATGCTGCCTGCGATTGCCCCCAGCCGGCCCTGG
GCCCTCATGGAGCAGTATGAGGTCGTGTTGCCGTGGCGTCTGCCAGGCCCCCGAGTCCGCCGAGCTCTG
CCCTCCCACTTGGGCCTGCACCCAGAGAGGGTGAGCTACGTCCTTGGGGCCACAGGGCACAACTTCACC
CTCCACCTGCGGAAGAACAGGGACCTGCTGGGCTCCGGCTACACAGAGACCTATACGGCTGCCAATGGC
TCCGAGGTGACGGAGCAGCCTCGCGGGCAGGACCACTGCTTCTACCAGGGCCACGTAGAGGGGTACCCG
GACTCAGCCGCCAGCCTCAGCACCTGTGCCGGCCTCAGGGGTTTCTTCCAGGTGGGGTCAGACCTGCAC
CTGATCGAGCCCCTGGATGAAGGTGGCGAGGGCGGACGGCACGCCGTGTACCAGGCTGAGCACCTGCTG
CAGACGGCCGGGACCTGCGGGGTCAGCGACGACAGCCTGGGCAGCCTCCTGGGACCCCGGACGGCAGCC
GTCTTCAGGCCTCGGCCCGGGGACTCTCTGCCATCCCGAGAGACCCGCTACGTGGAGCTGTATGTGGTC
GTGGACAATGCAGAGTTCCAGATGCTGGGGAGCGAAGCAGCCGTGCGTCATCGGGTGCTGGAGGTGGTG
AATCACGTGGACAAGCTATATCAGAAACTCAACTTCCGTGTGGTCCTGGTGGGCCTGGAGATTTGGAAT
AGTCAGGACAGGTTCCACGTCAGCCCCGACCCCAGTGTCACACTGGAGAACCTCCTGACCTGGCAGGCA
CGGCAACGGACACGGCGGCACCTGCATGACAACGTACAGCTCATCACGGGTGTCGACTTCACCGGGACT
ACCGTGGGGTTTGCCAGGGTGTCCGCCATGTGCTCCCACAGCTCAGGGGCTGTGAACCAGGACCACAGC
AAGAACCCCGTGGGCGTGGCCTGTACCATGGCCCATGAGATGGGCCACAACCTGGGCATGGACCATGAT
GAGAACGTCCAGGGCTGCCGCTGCCAGGAACGCTTCGAGGCCGGCCGCTGCATCATGGCGGGCAGCATT
GGCTCCAGTTTCCCCAGGATGTTCAGTGACTGCAGCCAGGCCTACCTGGAGAGCTTTTTGGAGCGGCCG
CAGTCGGTGTGCCTCGCCAACGCCCCTGACCTCAGCCACCTGGTGGGCGGCCCCGTGTGTGGGAACCTG
TTTGTGGAGCGTGGGGAGCAGTGCGACTGCGGCCCCCCCGAGGACTGCCGGAACCGCTGCTGCAACTCT
ACCACCTGCCAGCTGGCTGAGGGGGCCCAGTGTGCGCACGGTACCTGCTGCCAGGAGTGCAAGGTGAAG
CCGGCTGGTGAGCTGTGCCGTCCCAAGAAGGACATGTGTGACCTCGAGGAGTTCTGTGACGGCCGGCAC
CCTGAGTGCCCGGAAGACGCCTTCCAGGAGAACGGCACGCCCTGCTCCGGGGGCTACTGCTACAACGGG
GCCTGTCCCACACTGGCCCAGCAGTGCCAGGCCTTCTGGGGGCCAGGTGGGCAGGCTGCCGAGGAGTCC
TGCTTCTCCTATGACATCCTACCAGGCTGCAAGGCCAGCCGGTACAGGGCTGACATGTGTGGCGTTCTG
CAGTGCAAGGGTGGGCAGCAGCCCCTGGGGCGTGCCATCTGCATCGTGGATGTGTGCCACGCGCTCACC
ACAGAGGATGGCACTGCGTATGAACCAGTGCCCGAGGGCACCCGGTGTGGACCAGAGAAGGTTTGCTGG
AAAGGACGTTGCCAGGACTTACACGTTTACAGATCCAGCAACTGCTCTGCCCAGTGCCACAACCATGGG
GTGTGCAACCACAAGCAGGAGTGCCACTGCCACGCGGGCTGGGCCCCGCCCCACTGCGCGAAGCTGCTG
ACTGAGGTGCACGCAGGCTGCCAGCCGCGTGCCGGCCAAGGGCGGGGCTCCAGCCCCATCCAGGGGCCC
CCAAGAGCTGGTCCCCACCACCCACCCGGGCCAGCCCGCCCGACACCCGGCCTCCTCGGTGGCTCTGAA
GAGGCCGCCCCCTGCTCCTCCGGTCACTGTGTCCAGCCCACCCTTCCCAGTTCCTGTCTACACCCGGCA
GGCACCAAAGCAGGTCATCAAGCCAACGTTCGCACCCCCAGTGCCCCCAGTCAAACCCGGGGCTGGTGC
GGCCAACCCTGGTCCAGCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001164489
Insert Size 2229 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164489.1
RefSeq Size 3149 bp
RefSeq ORF 2229 bp
Locus ID 101
UniProt ID P78325
Cytogenetics 10q26.3
Protein Families Druggable Genome, Transmembrane
MW 80.3 kDa
Summary This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2009
Transcript Variant: This variant (2) lacks an alternate exon and uses an alternate splice site, which causes a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1.
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Documents
Resources
"NM_001164489" in other vectors (4)
SKU Description Size Price
RC228568 ADAM8 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 8 (ADAM8), transcript variant 2 10 ug
$679.00
RC228568L3 Lenti ORF clone of Human ADAM metallopeptidase domain 8 (ADAM8), transcript variant 2, Myc-DDK-tagged 10 ug
$979.00
RC228568L4 Lenti ORF clone of Human ADAM metallopeptidase domain 8 (ADAM8), transcript variant 2, mGFP tagged 10 ug
$979.00
RG228568 ADAM8 (tGFP-tagged) - Human ADAM metallopeptidase domain 8 (ADAM8), transcript variant 2 10 ug
$789.00 $879.00

file-alt Citations

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