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UPF1 (NM_002911) Human Untagged Clone

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SKU
SC323999
UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)
  $974.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UPF1
Synonyms HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_002911.3 GGCGACGGCGGCGGTGGCGGCAGTTCCTGCTCTAGGCTGCGAGCGGCTGGCGGCTTCGAG
GGGAGCTGAGGCGCGGAGGGGCTCGGCGGCAGCGGCGGCGGCTCGGCACTGTTACCTCTC
GGTCCGGCTGGCGCCGGGGCGGGCGGTTTGGTCCTTTCCGGGCGCGCGGGGGCGACAGCG
GCAGCGACCCGAGGCCTGCGGCCTAGGCCTCAGCGCGGCGGCGGGCTCGAGTGCAGCGCG
GAACCGGCCCGAGGGCCCTACCCGGAGGCACCATGAGCGTGGAGGCGTACGGGCCCAGCT
CGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTGCTTGGCGCCGACACACAGG
GCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACGCCCCCCGGCG
GCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGGGCGCTG
CGGCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGGCTGTGG
ACGACAGTGTAGCCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAGATGAAG
AAGACACCTATTACACGAAGGACCTCCCCATACACGCCTGCAGTTACTGTGGAATACACG
ATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGCAACGGACGTGGAA
ATACTTCTGGCAGCCACATTGTAAATCACCTTGTGAGGGCAAAATGCAAAGAGGTGACCC
TGCACAAGGACGGGCCCCTGGGGGAGACAGTCCTGGAGTGCTACAACTGCGGCTGTCGCA
ACGTCTTCCTCCTCGGCTTCATCCCGGCCAAAGCTGACTCAGTGGTGGTGCTGCTGTGCA
GGCAGCCCTGTGCCAGCCAGAGCAGCCTCAAGGACATCAACTGGGACAGCTCGCAGTGGC
AGCCGCTGATCCAGGACCGCTGCTTCCTGTCCTGGCTGGTCAAGATCCCCTCCGAGCAGG
AGCAGCTGCGGGCACGCCAGATCACGGCACAGCAGATCAACAAGCTGGAGGAGCTGTGGA
AGGAAAACCCTTCTGCCACGCTGGAGGACCTGGAGAAGCCGGGGGTGGACGAGGAGCCGC
AGCATGTCCTCCTGCGGTACGAGGACGCCTACCAGTACCAGAACATATTCGGGCCCCTGG
TCAAGCTGGAGGCCGACTACGACAAGAAGCTGAAGGAGTCCCAGACTCAAGATAACATCA
CTGTCAGGTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTACTTCACTTTGCCCAAGA
CTGACTCTGACATGCGGCTCATGCAGGGGGATGAGATATGCCTGCGGTACAAAGGGGACC
TTGCGCCCCTGTGGAAAGGGATCGGCCACGTCATCAAGGTCCCTGATAATTATGGCGATG
AGATCGCCATTGAGCTGCGGAGCAGCGTGGGTGCACCTGTGGAGGTGACTCACAACTTCC
AGGTGGATTTTGTGTGGAAGTCGACCTCCTTTGACAGGATGCAGAGCGCATTGAAAACGT
TTGCCGTGGATGAGACCTCGGTGTCTGGCTACATCTACCACAAGCTGTTGGGCCACGAGG
TGGAGGACGTAATCATCAAGTGCCAGCTGCCCAAGCGCTTCACGGCGCAGGGCCTCCCCG
ACCTCAACCACTCCCAGGTTTATGCCGTGAAGACTGTGCTGCAAAGACCACTGAGCCTGA
TCCAGGGCCCGCCAGGCACGGGGAAGACGGTGACGTCGGCCACCATCGTCTACCACCTGG
CCCGGCAAGGCAACGGGCCGGTGCTGGTGTGTGCTCCGAGCAACATCGCCGTGGACCAGC
TAACGGAGAAGATCCACCAGACGGGGCTAAAGGTCGTGCGCCTCTGCGCCAAGAGCCGTG
AGGCCATCGACTCCCCGGTGTCTTTTCTGGCCCTGCACAACCAGATCAGGAACATGGACA
GCATGCCTGAGCTGCAGAAGCTGCAGCAGCTGAAAGACGAGACTGGGGAGCTGTCGTCTG
CCGACGAGAAGCGGTACCGGGCCTTGAAGCGCACCGCAGAGAGAGAGCTGCTGATGAACG
CAGATGTCATCTGCTGCACATGTGTGGGCGCCGGTGACCCGAGGCTGGCCAAGATGCAGT
TCCGCTCCATTTTAATCGACGAAAGCACCCAGGCCACCGAGCCGGAGTGCATGGTTCCCG
TGGTCCTCGGGGCCAAGCAGCTGATCCTTGTAGGCGACCACTGCCAGCTGGGCCCAGTGG
TGATGTGCAAGAAGGCGGCCAAGGCCGGGCTGTCACAGTCGCTCTTCGAGCGCCTGGTGG
TGCTGGGCATCCGGCCCATCCGCCTGCAGGTCCAGTACCGGATGCACCCTGCACTCAGCG
CCTTCCCATCCAACATCTTCTACGAGGGCTCCCTCCAGAATGGTGTCACTGCAGCGGATC
GTGTGAAGAAGGGATTTGACTTCCAGTGGCCCCAACCCGATAAACCGATGTTCTTCTACG
TGACCCAGGGCCAAGAGGAGATTGCCAGCTCGGGCACCTCCTACCTGAACAGGACCGAGG
CTGCGAACGTGGAGAAGATCACCACGAAGTTGCTGAAGGCAGGCGCCAAGCCGGACCAGA
TTGGCATCATCACGCCCTACGAGGGCCAGCGCTCCTACCTGGTGCAGTACATGCAGTTCA
GCGGCTCCCTGCACACCAAGCTCTACCAGGAGGTGGAGATCGCCAGTGTGGACGCCTTTC
AGGGACGCGAGAAGGACTTCATCATCCTGTCCTGTGTGCGGGCCAACGAGCACCAAGGCA
TTGGCTTTTTAAATGACCCCAGGCGTCTGAACGTGGCCCTGACCAGAGCAAGGTATGGCG
TCATCATTGTGGGCAACCCGAAGGCACTATCAAAGCAGCCGCTCTGGAACCACCTGCTGA
ACTACTATAAGGAGCAGAAGGTGCTGGTGGAGGGGCCGCTCAACAACCTGCGTGAGAGCC
TCATGCAGTTCAGCAAGCCACGGAAGCTGGTCAACACTATCAACCCGGGAGCCCGCTTCA
TGACCACAGCCATGTATGATGCCCGGGAGGCCATCATCCCAGGCTCCGTCTATGATCGGA
GCAGCCAGGGCCGGCCTTCCAGCATGTACTTCCAGACCCATGACCAGATTGGCATGATCA
GTGCCGGCCCTAGCCACGTGGCTGCCATGAACATTCCCATCCCCTTCAACCTGGTCATGC
CACCCATGCCACCGCCTGGCTATTTTGGACAAGCCAACGGGCCTGCTGCAGGGCGAGGCA
CCCCGAAAGGCAAGACTGGTCGTGGGGGACGCCAGAAGAACCGCTTTGGGCTTCCTGGAC
CCAGCCAGACTAACCTCCCCAACAGCCAAGCCAGCCAGGATGTGGCGTCACAGCCCTTCT
CTCAGGGCGCCCTGACGCAGGGCTACATCTCCATGAGCCAGCCTTCCCAGATGAGCCAGC
CCGGCCTCTCCCAGCCGGAGCTGTCCCAGGACAGTTACCTTGGTGACGAGTTTAAATCAC
AAATCGACGTGGCGCTCTCACAGGACTCCACGTACCAGGGAGAGCGGGCTTACCAGCATG
GCGGGGTGACGGGGCTGTCCCAGTATTAAAAGGTGGCGGCGGAAGAGCTAAGCAACGTGG
CTTAGTCCATCAGCATCTTATTCTGGGTAATAAAAAATAAAAATAAACGGATACCTGTTT
TCCACTGCTAAAACTGAAGCACCACTGTGTGAGCAACAGGAAGGGAGAGCGCACGAGGGA
GAGGAGCCGAGGCCGAGCGCCCCCTGCTGGCCCGCGGCGGCGAGGAGCAGAGGGAGCGGA
GGAGGGGCCGGCCCGCGGGAGCCGCGGCCACCAGGAGGCCCCGCTCCGTCCCATCGGGGC
TGCGGCCAGGGCGGAGGGAGGAAGACCCTCATCTCAGAGTAGCCCTTTCCTCTGTTCTTT
TATTTCTTTTTCTCTTTGATTGAAAGGGGACTACGTCTTAGCAGGAAAAAAAACTTCGCA
TTTCTGTGCCCGAGCAGGCTCCTTGCAAAGACAGCAGCGTGCGGGGCAGAGCCCCGGGAG
GGCGCGTCTGTCCACGCCTACCGGACGCGCCGAGGTCGCGCTGCCTGTGTTCTCCGAGGG
CCTTCATTTAAAGAAAATAAGGGTGTTTTGGGTTTTTCTCTTTGTTTTTTTCAAGATTCT
TTTAAAGGAGTACTGAAGAATACTTTCCTAAGTTTGTCTCTAAAATCTTAGCGGTGGACC
TGGGAGATTTGAGAAGCTTCCAGAAACAGTTTAAACAAGCCAGCGCTACTGGAGAAGAGG
AGCAACACCTGTGCCGCGGCCGGAGGAGTTTTGTTGTTGGTTTTAGCTTCCAGTGGCTTC
TTTCTGCGGGGCATCAGGCTGCTGGGGTAGCCGCCCGCCGAGCCTGGAAGCTGCTCGTTC
TCCGCTGGACTCAGAAGCCAAGCTGCTTCCCGCCTAGACTCGGCGCAGGGCCCCGCACCG
GTGAGGAAGGTGCTTTTGGCCCCATTGCGAGGGGCCTTGGCCAGGACTGGCCCTGTGGCC
AGGAGGCGAGAAGGTGGCTGTTCCCGGATTGACGGCTTTTTCCCGGGGGCCTTTGGAAGA
TTTGGTGGAAGGACAAGAGGGCCTGTCCCTGTCCCCGTCCCCAGGAGGTACCGACAGTCC
CTGTGCTGGTTAGACACGGAGCGCTGCACACCGAAAGCCCAAATTGGGAGCTCTGCCTGC
CGGCAACTTTGCTGATGGGGTGATTGCTGCTTCTGGGGGGTAAGGAAACAAGTTACAGAA
ATTACCGCGTTCTGTGTGAAGGGACTGAGGGTGTGGTGTCATTGGCAGAGGGTCATTTTA
GGAGAGCTGCCCCAGCCCCTCGAACGCCTGGCTTGGGGTGTCATTCTGCCTGGCGGCCAG
GCCTCCAGCTTCCCCTGCCCCGGGCCTGGGGCTGTCACTGGCCCTGATCCGAACACCTCC
AGATTCCGGCTTCTACATGGGACAGACGGGGACGCACAGGCCACCTTCCTTCTGGCAGGG
ACTCTTATTTATTCCCATTGCTCTAGGGCTTTCGGTTTCCCCTTCTTCCGGTAGGCCGCG
TAGAGGCATGCACCGGGTAGGTTTCCGCGGTGACCCCGCGGCGGCCTGAGGGACGCTCCC
TGCCCCATCCCGGCTGTTGGGCTGGGCCGCTTTGCCTCTGCTTCGCCCTGTGCTGTGTTC
TCCAGCTTTGTAGCAGCAGCCTTGACAAACCCAGGCGCACTGTACCAAGGCAATGTAACT
TTTGATTTTCGGTCAATTTAAGTTCTTTTGTCACCAAATATTAATAAACAGTTTTGACTT
CAAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_002911
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002911.3, NP_002902.2
RefSeq Size 5360 bp
RefSeq ORF 3357 bp
Locus ID 5976
UniProt ID Q92900
Cytogenetics 19p13.11
Protein Families Druggable Genome
Summary This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2014
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.
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"NM_002911" in other vectors (7)
SKU Description Size Price
RC208018 UPF1 (Myc-DDK-tagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) 10 ug
$1,459.00
RC208018L1 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged 10 ug
$1,759.00
RC208018L2 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged 10 ug
$1,759.00
RC208018L3 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged 10 ug
$1,759.00
RC208018L4 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged 10 ug
$1,759.00
RG208018 UPF1 (tGFP-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) 10 ug
$1,659.00
SC118343 UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) 10 ug
$974.00

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