OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

UPF1 (NM_002911) Human Untagged Clone

Be the first to review this product
SKU
SC118343
UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)
  $974.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA350889 Rabbit Polyclonal Anti-UPF1 Antibody
100 ul
$205.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "UPF1 or NM_002911"

Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UPF1
Synonyms HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118343 sequence for NM_002911 edited (data generated by NextGen Sequencing)
ATGAGCGTGGAGGCGTACGGGCCCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAG
GCCGAGCTGCTTGGCGCCGACACACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTT
CCTAGCCAGACGCAGACGCCCCCCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGA
GGCCCGGGCGGCGCGGGCGCGGGCGCTGCGGCGGGACAGCTCGACGCGCAGGTTGGGCCC
GAAGGCATCCTGCAGAACGGGGCTGTGGACGACAGTGTAGCCAAGACCAGCCAGTTGTTG
GCTGAGTTGAACTTCGAGGAAGATGAAGAAGACACCTATTACACGAAGGACCTCCCCATA
CACGCCTGCAGTTACTGTGGAATACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGC
AAGAAGTGGTTCTGCAACGGACGTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTT
GTGAGGGCAAAATGCAAAGAGGTGACCCTGCACAAGGACGGGCCCCTGGGGGAGACAGTC
CTGGAGTGCTACAACTGCGGCTGTCGCAACGTCTTCCTCCTCGGCTTCATCCCGGCCAAA
GCTGACTCAGTGGTGGTGCTGCTGTGCAGGCAGCCCTGTGCCAGCCAGAGCAGCCTCAAG
GACATCAACTGGGACAGCTCGCAGTGGCAGCCGCTGATCCAGGACCGCTGCTTCCTGTCC
TGGCTGGTCAAGATCCCCTCCGAGCAGGAGCAGCTGCGGGCACGCCAGATCACGGCACAG
CAGATCAACAAGCTGGAGGAGCTGTGGAAGGAAAACCCTTCTGCCACGCTGGAGGACCTG
GAGAAGCCGGGGGTGGACGAGGAGCCGCAGCATGTCCTCCTGCGGTACGAGGACGCCTAC
CAGTACCAGAACATATTCGGGCCCCTGGTCAAGCTGGAGGCCGACTACGACAAGAAGCTG
AAGGAGTCCCAGACTCAAGATAACATCACTGTCAGGTGGGACCTGGGCCTTAACAAGAAG
AGAATCGCCTACTTCACTTTGCCCAAGACTGACTCTGACATGCGGCTCATGCAGGGGGAT
GAGATATGCCTGCGGTACAAAGGGGACCTTGCGCCCCTGTGGAAAGGGATCGGCCACGTC
ATCAAGGTCCCTGATAATTATGGCGATGAGATCGCCATTGAGCTGCGGAGCAGCGTGGGT
GCACCTGTGGAGGTGACTCACAACTTCCAGGTGGATTTTGTGTGGAAGTCGACCTCCTTT
GACAGGATGCAGAGCGCATTGAAAACGTTTGCCGTGGATGAGACCTCGGTGTCTGGCTAC
ATCTACCACAAGCTGTTGGGCCACGAGGTGGAGGACGTAATCATCAAGTGCCAGCTGCCC
AAGCGCTTCACGGCGCAGGGCCTCCCCGACCTCAACCACTCCCAGGTTTATGCCGTGAAG
ACTGTGCTGCAAAGACCACTGAGCCTGATCCAGGGCCCGCCAGGCACGGGGAAGACGGTG
ACGTCGGCCACCATCGTCTACCACCTGGCCCGGCAAGGCAACGGGCCGGTGCTGGTGTGT
GCTCCGAGCAACATCGCCGTGGACCAGCTAACGGAGAAGATCCACCAGACGGGGCTAAAG
GTCGTGCGCCTCTGCGCCAAGAGCCGTGAGGCCATCGACTCCCCGGTGTCTTTTCTGGCC
CTGCACAACCAGATCAGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTG
AAAGACGAGACTGGGGAGCTGTCGTCTGCCGACGAGAAGCGGTACCGGGCCTTGAAGCGC
ACCGCAGAGAGAGAGCTGCTGATGAACGCAGATGTCATCTGCTGCACATGTGTGGGCGCC
GGTGACCCGAGGCTGGCCAAGATGCAGTTCCGCTCCATTTTAATCGACGAAAGCACCCAG
GCCACCGAGCCGGAGTGCATGGTTCCCGTGGTCCTCGGGGCCAAGCAGCTGATCCTTGTA
GGCGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCGGCCAAGGCCGGGCTG
TCACAGTCGCTCTTCGAGCGCCTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTC
CAGTACCGGATGCACCCTGCACTCAGCGCCTTCCCATCCAACATCTTCTACGAGGGCTCC
CTCCAGAATGGTGTCACTGCAGCGGATCGTGTGAAGAAGGGATTTGACTTCCAGTGGCCC
CAACCCGATAAACCGATGTTCTTCTACGTGACCCAGGGCCAAGAGGAGATTGCCAGCTCG
GGCACCTCCTACCTGAACAGGACCGAGGCTGCGAACGTGGAGAAGATCACCACGAAGTTG
CTGAAGGCAGGCGCCAAGCCGGACCAGATTGGCATCATCACGCCCTACGAGGGCCAGCGC
TCCTACCTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACCAAGCTCTACCAGGAG
GTGGAGATCGCCAGTGTGGACGCCTTTCAGGGACGCGAGAAGGACTTCATCATCCTGTCC
TGTGTGCGGGCCAACGAGCACCAAGGCATTGGCTTTTTAAATGACCCCAGGCGTCTGAAC
GTGGCCCTGACCAGAGCAAGGTATGGCGTCATCATTGTGGGCAACCCGAAGGCACTATCA
AAGCAGCCGCTCTGGAACCACCTGCTGAACTACTATAAGGAGCAGAAGGTGCTGGTGGAG
GGGCCGCTCAACAACCTGCGTGAGAGCCTCATGCAGTTCAGCAAGCCACGGAAGCTGGTC
AACACTATCAACCCGGGAGCCCGCTTCATGACCACAGCCATGTATGATGCCCGGGAGGCC
ATCATCCCAGGCTCCGTCTATGATCGGAGCAGCCAGGGCCGGCCTTCCAGCATGTACTTC
CAGACCCATGACCAGATTGGCATGATCAGTGCCGGCCCTAGCCACGTGGCTGCCATGAAC
ATTCCCATCCCCTTCAACCTGGTCATGCCACCCATGCCACCGCCTGGCTATTTTGGACAA
GCCAACGGGCCTGCTGCAGGGCGAGGCACCCCGAAAGGCAAGACTGGTCGTGGGGGACGC
CAGAAGAACCGCTTTGGGCTTCCTGGACCCAGCCAGACTAACCTCCCCAACAGCCAAGCC
AGCCAGGATGTGGCGTCACAGCCCTTCTCTCAGGGCGCCCTGACGCAGGGCTACATCTCC
ATGAGCCAGCCTTCCCAGATGAGCCAGCCCGGCCTCTCCCAGCCGGAGCTGTCCCAGGAC
AGTTACCTTGGTGACGAGTTTAAATCACAAATCGACGTGGCGCTCTCACAGGACTCCACG
TACCAGGGAGAGCGGGCTTACCAGCATGGCGGGGTGACGGGGCTGTCCCAGTATTAA

Clone variation with respect to NM_002911.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002911 unedited
CCGCGGAATTCGCACGAGGCCGGGGCGGGCGGTTTGGTCCTTTCCGGGCGCGCGGGGGCG
ACAGCGGCAGCGACCCGAGGCCTGCGGCCTAGGCCTCAGCGCGGCGGCGGGCTCGAGTGC
AGCGCGGAACCGGCCCGAGGGCCCTACCCGGAGGCACCATGAGCGTGGAGGCGTACGGGC
CCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTGCTTGGCGCCGACA
CACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACGCCCC
CCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGG
GCGCTGCGGCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGG
CTGTGGACGACAGTGTAGCCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAG
ATGAAGAAGACACCTATTACACGAAGGACCTCCCCATACACGCCTGCAGTTACTGTGGAA
TACACGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGCAACGGAC
GTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTTGTGAGGGCAAAATGCAAAGAGG
TGACCCTGCACAAGGACGGGGCCCCTGGGGGAGACAGTCCCTGGAGTGCTACAACTGGCG
GCTGTCGCAACGTCTTTCTCCTCGGCTTCATCCCGGGCAAAGCTGACTCAATGGGTGGGG
GCTGCTGTGCAGGCAACCCCTGTGCCAACCCGAGCAGCCTCAAAGGACATAACTGGGACA
ACTCGCAGTGGCAGACCGGTGATCCAGGAACGCGGGTTCCTGTCCTGGGTTGGGCAAAGA
ACCCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002911 unedited
AACGTAGGGGAAACAGGTATCCGTTTTTTTTTATTTTTTATTACCCAGNATAAGATGCTG
ATGGACTAAGCCACGTTGCTTAGCTCTTCCGCCGCCACCTTTTAATACTGGGACAGCCCC
GTCACCCCGCCATGCTGGTAAGCCCGCTCTCCCTGGTACGTGGAGTCCTGTGAGAGCGCC
ACGTCGATTTGTGATTTAAACTCGTCACCAAGGTAACTGTCCTGGGACAGCTCCGGCTGG
GAGAGGCCGGGCTGGCTCATCTGGGAAGGCTGGCTCATGGAGATGTAGCCCTGCGTCAGG
GCGCCCTGAGAGAAGGGCTGTGACGCCACATCCTGGCTGGCTTGGCTGTTGGGGAGGTTA
GTCTGGCTGGGTCCAGGAAGCCCAAAGCGGTTCTTCTGGCGTCCCCCACGACCAGTCTTG
CCTTTCGGGGTGCCTCGCCCTGCAGCAGGCCCGTTGGCTTGTCCAAAATAGCCAGGCGGT
GGCATGGGTGGCATGACCAGGTTGAAGGGGATGGGAATGTTCATGGCAGCCACGTGGCTA
GGGCCGGCACTGATCATGCCAATCTGGTCATGGGTCTGGAAGTACATGCTGGAAGGCCGG
CCCTGGCTGCTCCGATCATAGACGGAGCCTGGGATGATGGCCTCCCGGGCATCATACATG
GCTGTGGTCATGAAGCGGGCTCCCGGTTGATAGTGTTGACCAGCTTCCGTGGCTTGCTGA
ACTGCATGAGGCTTTCACCGCAGTTGTTGAGCGGGCCCTTCAACAGCACCCTTTTGTTCC
TTATAGTAGTTCAGCAGGTGGGTTTCAGAGCCGGCTGCTTTGTTAAGTGCTCTCGGTTGC
CCCCAATGATGAACCCCTAACCTTGCTCTGGTCAGGGCCACCCTCAACCCCCTTGGGTTA
TTTTAAAAAACCATGGCTCTGGGCTTTCTTGCCCCACAACGGGAAGGT
Restriction Sites ECoRI-NOT |
ACCN NM_002911
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002911.2, NP_002902.2
RefSeq Size 5300 bp
RefSeq ORF 3357 bp
Locus ID 5976
UniProt ID Q92900
Cytogenetics 19p13.11
Protein Families Druggable Genome
Summary This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2014
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_002911" in other vectors (7)
SKU Description Size Price
RC208018 UPF1 (Myc-DDK-tagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) 10 ug
$1,459.00
RC208018L1 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged 10 ug
$1,759.00
RC208018L2 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged 10 ug
$1,759.00
RC208018L3 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), Myc-DDK-tagged 10 ug
$1,759.00
RC208018L4 Lenti ORF clone of Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), mGFP tagged 10 ug
$1,759.00
RG208018 UPF1 (tGFP-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) 10 ug
$1,659.00
SC323999 UPF1 (untagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) 10 ug
$974.00

file-alt Citations

The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition
Moldovan, Moran
PLoS Genet. (2015) 11 (5), e1005121 DOI: 10.1371/journal.pgen.1005121
Cited by 149
AI Summary: OriGene contributed cDNA expression plasmids for several proteins—including CDK9, DDX21, GNB2L1, hnRNPDL, MOV10, MATR3, ZAP-S (ZC3HAV1 transcript variant 2), ZAP-S-tGFP, hnRNPA2B1, IGF2BP3, PURA, and UPF1—which were used in this study to investigate protein interactions with L1 ORF1p and the role of ZAP in inhibiting LINE and Alu retrotransposition.
Snippet: The following cDNA expression plasmids were obtained from OriGene: CDK9 (SC119344);DDX21 (SC108813);GNB2L1 (SC116322);hnRNPDL (SC107613);MOV10 (SC126015);MATR3 (SC113375);ZAP-S (ZC3HAV1 transcript variant 2) (SC101064);ZAP-S-tGFP (GFP-tagged ZC3HAV1 transcript variant 2) (RG208070);hnRNPA2B1 (SC313092);IGF2BP3 (SC111161);PURA (SC127792);UPF1 (SC118343)
FigureFigureFigure
A luciferase reporter assay to investigate the differential selenium-dependent stability of selenoprotein mRNAs
Banerjee, Yang, Foster
J. Nutr. Biochem. (2012) 23 (10), 1294-301 DOI: 10.1016/j.jnutbio.2011.07.010
Cited by 9
AI Summary: OriGene products, specifically the UPF1 shRNA pool (TR308482) and GFP-specific control shRNA plasmid, were used to modulate UPF1 expression in HeLa cells, enabling the authors to assess the role of UPF1 in nonsense-mediated decay within their luciferase reporter assay.
Snippet: 2.8. UPF1 knockdown and overexpression. To knock down the expression of UPF1, HeLa cells were transiently transfected with a pool of four UPF1 shRNAs (Origene;TR308482) or a GFP-specific control shRNA plasmid (Origene).
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products