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SF3B3 (NM_012426) Human Untagged Clone

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SKU
SC323872
SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)
  $1,060.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SF3B3
Synonyms RSE1; SAP130; SF3b130; STAF130
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_012426.3 TGTTGGAGTTGGTGGTGGCTTAAGTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACAC
CATCCTTCTCGCTGCAGGCTTTCTTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAG
GTCTGGGTGGCTCAGGTTTCCTGCAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGC
CACTGGCATCAGCTTTGCCATTCATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGT
TGTTTCCCGTGGGAAGATCTTGGAGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACA
TACCCTACTCACTGTGGAAGTATTCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGAC
AGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGA
ATACCAGCCATCTAAGAATATGTTTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGG
ATGCCGTCGCATCGTTCCTGGCCAGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTAT
GATTAGTGCCATTGAGAAACAGAAATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCG
ACTTACCATTTCATCTCCCCTGGAAGCCCACAAAGCAAACACTTTAGTGTATCATGTAGT
TGGAGTAGATGTCGGATTTGAAAATCCAATGTTTGCTTGTCTGGAAATGGATTATGAGGA
AGCAGACAATGATCCAACAGGGGAAGCAGCAGCTAATACCCAGCAGACACTTACTTTCTA
TGAGCTAGACCTTGGTTTAAATCATGTGGTCCGAAAATACAGTGAACCTTTGGAGGAACA
CGGCAACTTCCTTATTACAGTTCCAGGAGGGTCAGATGGTCCAAGTGGAGTACTGATCTG
CTCTGAAAACTATATTACTTACAAGAACTTTGGTGACCAGCCAGATATCCGCTGTCCAAT
TCCCAGGAGGCGGAATGACCTGGATGACCCTGAAAGAGGAATGATTTTTGTCTGCTCTGC
AACCCATAAAACCAAATCGATGTTCTTCTCTTTGGCTCAAACTGAGCAGGGAGATATCTT
TAAGATCACTTTGGAGACAGATGAAGATATGGTTACTGAGATCCGGCTCAAATATTTTGA
TACTGTACCCGTTGCTGCTGCCATGTGTGTGCTTAAAACAGGGTTCCTTTTTGTAGCATC
AGAATTTGGAAACCATTACTTATATCAAATTGCACATCTTGGAGATGATGATGAAGAACC
TGAGTTTTCATCAGCCATGCCTCTGGAAGAAGGAGACACATTCTTTTTTCAGCCAAGACC
ACTTAAAAACCTTGTGCTGGTTGATGAGTTGGACAGCCTCTCTCCCATTCTGTTTTGCCA
GATAGCTGATCTGGCCAATGAAGATACTCCACAGTTGTATGTGGCCTGTGGTAGGGGACC
CCGATCATCTCTGAGAGTCCTAAGACATGGACTTGAGGTGTCAGAAATGGCTGTTTCTGA
GCTACCTGGTAACCCCAACGCTGTCTGGACAGTGCGTCGACACATTGAAGATGAGTTTGA
TGCCTACATTATTGTGTCTTTCGTGAATGCCACCCTAGTGTTGTCCATTGGAGAAACTGT
AGAAGAAGTGACTGACTCTGGGTTCCTGGGGACCACCCCGACCTTGTCCTGCTCCTTATT
AGGAGATGATGCCTTGGTGCAGGTCTATCCAGATGGCATTCGGCACATACGAGCAGACAA
GAGAGTCAATGAGTGGAAGACCCCTGGAAAGAAAACAATTGTGAAGTGTGCAGTGAACCA
GCGACAAGTGGTGATTGCCCTGACAGGAGGAGAGCTGGTCTATTTCGAGATGGATCCTTC
AGGACAGCTGAATGAGTACACAGAACGGAAGGAGATGTCAGCAGATGTGGTGTGCATGAG
TCTGGCCAATGTACCCCCTGGAGAGCAGCGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGA
CAACACTGTCAGAATCATCTCCCTGGATCCCTCAGACTGTTTGCAACCTCTAAGCATGCA
GGCTCTCCCAGCCCAGCCTGAGTCCTTGTGTATCGTGGAAATGGGTGGGACTGAGAAGCA
GGATGAGCTGGGTGAGAGGGGCTCGATTGGCTTCCTATACCTGAATATTGGGCTACAGAA
CGGTGTGCTGCTGAGGACTGTCTTGGACCCTGTCACTGGGGATTTGTCTGATACTCGCAC
TCGGTACCTGGGGTCCCGTCCTGTGAAGCTCTTCCGAGTCCGAATGCAAGGCCAGGAGGC
AGTATTGGCCATGTCAAGCCGCTCATGGTTGAGCTATTCTTACCAATCTCGCTTCCATCT
CACCCCACTGTCTTACGAGACACTGGAATTTGCATCGGGTTTTGCCTCGGAACAGTGTCC
CGAGGGCATTGTGGCCATCTCCACCAACACCCTACGGATTTTGGCATTAGAGAAGCTCGG
TGCTGTCTTCAATCAAGTAGCCTTCCCACTGCAGTACACACCCAGGAAATTTGTCATCCA
CCCTGAGAGTAACAACCTTATTATCATTGAAACGGACCACAATGCCTACACTGAGGCCAC
GAAAGCTCAGAGAAAGCAGCAGATGGCAGAGGAAATGGTGGAAGCAGCAGGGGAGGATGA
GCGGGAGCTGGCCGCAGAGATGGCAGCAGCATTCCTCAATGAAAACCTCCCTGAATCCAT
CTTTGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCTCTGTGATCCGAGTGATGAATCC
CATTCAAGGGAACACACTGGACCTTGTCCAGCTGGAACAGAATGAGGCAGCTTTTAGTGT
GGCTGTGTGCAGGTTTTCCAACACTGGTGAAGACTGGTATGTGCTGGTGGGTGTGGCCAA
GGACCTGATACTAAACCCCCGATCTGTGGCAGGGGGCTTCGTCTATACTTACAAGCTTGT
GAACAATGGGGAAAAACTGGAGTTTTTGCACAAGACTCCTGTGGAAGAGGTCCCTGCTGC
TATTGCCCCATTCCAGGGGAGGGTGTTGATTGGTGTGGGGAAGCTGTTGCGTGTCTATGA
CCTGGGAAAGAAGAAGTTACTCCGAAAATGTGAGAATAAGCATATTGCCAATTATATCTC
TGGGATCCAGACTATCGGACATAGGGTAATTGTATCTGATGTCCAAGAAAGTTTCATCTG
GGTTCGCTACAAGCGTAATGAAAACCAGCTTATCATCTTTGCTGATGATACCTACCCCCG
ATGGGTCACTACAGCCAGCCTCCTGGACTATGACACTGTGGCTGGGGCAGACAAGTTTGG
CAACATATGTGTGGTGAGGCTCCCACCTAACACCAATGATGAAGTAGATGAGGATCCTAC
AGGAAACAAAGCCCTGTGGGACCGTGGCTTGCTCAATGGGGCCTCCCAGAAGGCAGAGGT
GATCATGAACTACCATGTCGGGGAGACGGTGCTGTCCTTGCAGAAGACCACGCTGATCCC
TGGAGGCTCAGAATCACTTGTCTATACCACCTTGTCTGGAGGAATTGGCATCCTTGTGCC
ATTCACGTCCCATGAGGACCATGACTTCTTCCAGCATGTGGAAATGCACCTGCGGTCTGA
ACATCCCCCTCTCTGTGGGCGGGACCACCTCAGCTTTCGCTCCTACTACTTCCCTGTGAA
GAATGTGATTGATGGAGACCTCTGTGAGCAGTTCAATTCCATGGAACCCAACAAACAAAA
GAACGTCTCTGAAGAACTGGACCGAACCCCACCCGAAGTGTCCAAGAAACTCGAGGATAT
CCGGACCCGCTACGCCTTCTGAGCCCTCCTTTCCCGGTGGGGCTTGCCAGAGACTGTGTG
TTTTGTTTCCCCCACCACCATCACTGCCACCTGGCTTCTGCCATGTGGCAGGAGGGTGAC
TGGATAATTAAGACTGCATTATGAAAGTCAACAGCTCTTTCCCCTCAGCTCTTCTCCTGG
AATGACTGGCTTCCCCTCAAATTGGCACTGAGATTTGCTACACTTCTCCCCACCTGGTAC
ATGATACATGACCCCAGGTTCCAGTGTAGAACCTGAGTCCCCCATTCCCCAAAGCCATCC
CTGCATTGATATGTCTTGACTCTCCTGTCTACTTTTGCACACACCCTTAATTTTTAATTG
GTTTTCTTGTAAAAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_012426
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012426.3, NP_036558.3
RefSeq Size 4357 bp
RefSeq ORF 3654 bp
Locus ID 23450
UniProt ID Q15393
Cytogenetics 16q22.1
Domains CPSF_A
Protein Pathways Spliceosome
Summary This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. provided by RefSeq, Jul 2008
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Resources
"NM_012426" in other vectors (7)
SKU Description Size Price
RC209509 SF3B3 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) 10 ug
$1,059.00
RC209509L1 Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged 10 ug
$1,359.00
RC209509L2 Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged 10 ug
$1,359.00
RC209509L3 Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), Myc-DDK-tagged 10 ug
$1,359.00
RC209509L4 Lenti ORF clone of Human splicing factor 3b, subunit 3, 130kDa (SF3B3), mGFP tagged 10 ug
$1,359.00
RG209509 SF3B3 (tGFP-tagged) - Human splicing factor 3b, subunit 3, 130kDa (SF3B3) 10 ug
$789.00 $1,259.00
SC126731 SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3) 10 ug
$1,060.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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