SF3B3 (NM_012426) Human Untagged Clone

SKU SC126731

SF3B3 (untagged)-Human splicing factor 3b, subunit 3, 130kDa (SF3B3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SF3B3
• Synonyms: RSE1; SAP130; SF3b130; STAF130
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126731 sequence for NM_012426 edited (data generated by NextGen Sequencing); ATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTCATGGA; AACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGGAGCTG; CTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTATTCGGT; GTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTGTAGTT; GGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGTTTGAG; AAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCCAGTTC; TTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGAAATTG; GTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGGAAGCC; CACAAAGCAAACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAAATCCA; ATGTTTGCTTGTCTGGAAATGGATTATGAGGAAGCAGACAATGATCCAACAGGGGAAGCA; GCAGCTAATACCCAGCAGACACTTACTTTCTATGAGCTAGACCTTGGTTTAAATCATGTG; GTCCGAAAATACAGTGAACCTTTGGAGGAACACGGCAACTTCCTTATTACAGTTCCTGGA; GGGTCAGATGGTCCAAGTGGAGTACTGATCTGCTCTGAAAACTATATTACTTACAAGAAC; TTTGGTGACCAGCCAGATATCCGCTGTCCAATTCCCAGGAGGCGGAATGACCTGGATGAC; CCTGAAAGAGGAATGATTTTTGTCTGCTCTGCAACCCATAAAACCAAATCGATGTTCTTC; TTTTTGGCTCAAACTGAGCAGGGAGATATCTTTAAGATCACTTTGGAGACAGATGAAGAT; ATGGTTACTGAGATCCGGCTCAAATATTTTGATACTGTACCCGTTGCTGCTGCCATGTGT; GTGCTTAAAACAGGGTTCCTTTTTGTAGCATCAGAATTTGGAAACCATTACTTATATCAA; ATTGCACATCTTGGAGATGATGATGAAGAACCTGAGTTTTCATCAGCCATGCCTCTGGAA; GAAGGAGACACATTCTTTTTTCAGCCAAGACCACTTAAAAACCTTGTGCTGGTTGATGAG; TTGGACAGCCTCTCTCCCATTCTGTTTTGCCAGATAGCTGATCTGGCCAATGAAGATACT; CCACAGTTGTATGTGGCCTGTGGTAGGGGACCCCGATCATCTCTGAGAGTCCTAAGACAT; GGACTTGAGGTGTCAGAAATGGCTGTTTCTGAGCTACCTGGTAACCCCAACGCTGTCTGG; ACAGTGCGTCGACACATTGAAGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAAT; GCCACCCTAGTGTTGTCCATTGGAGAAACTGTAGAAGAAGTGACTGACTCTGGGTTCCTG; GGGACCACCCCGACCTTGTCCTGCTCCTTATTAGGAGATGATGCCTTGGTGCAGGTCTAT; CCAGATGGCATTCGGCACATACGAGCAGACAAGAGAGTCAATGAGTGGAAGACCCCTGGA; AAGAAAACAATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATTGCCCTGACAGGA; GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGCTGAATGAGTACACAGAACGG; AAGGAGATGTCAGCAGATGTGGTGTGCATGAGTCTGGCCAATGTACCCCCTGGAGAGCAG; CGGTCTCGCTTCCTGGCTGTGGGGCTTGTGGACAACACTGTCAGAATCATCTCCCTGGAT; CCCTCAGACTGTTTGCAACCTCTAAGCATGCAGGCTCTCCCAGCCCAGCCTGAGTCCTTG; TGTATCGTGGAAATGGGTGGGACTGAGAAGCAGGATGAGCTGGGTGAGAGGGGCTCGATT; GGCTTCCTATACCTGAATATTGGGCTACAGAACGGTGTGCTGCTGAGGACTGTCTTGGAC; CCTGTCACTGGGGATTTGTCTGATACTCGCACTCGGTACCTGGGGTCCCGTCCTGTGAAG; CTCTTCCGAGTCCGAATGCAAGGCCAGGAGGCAGTATTGGCCATGTCAAGCCGCTCATGG; TTGAGCTATTCTTACCAATCTCGCTTCCATCTCACCCCACTGTCTTACGAGACACTGGAA; TTTGCATCGGGTTTTGCCTCGGAACAGTGTCCCGAGGGCATTGTGGCCATCTCCACCAAC; ACCCTACGGATTTTGGCATTAGAGAAGCTCGGTGCTGTCTTCAATCAAGTAGCCTTCCCA; CTGCAGTACACACCCAGGAAATTTGTCATCCACCCTGAGAGTAACAACCTTATTATCATT; GAAACGGACCACAATGCCTACACTGAGGCCACGAAAGCTCAGAGAAAGCAGCAGATGGCA; GAGGAAATGGTGGAAGCAGCAGGGGAGGATGAGCGGGAGCTGGCCGCAGAGATGGCAGCA; GCATTCCTCAATGAAAACCTCCCTGAATCCATCTTTGGAGCTCCCAAGGCTGGCAATGGG; CAGTGGGCCTCTGTGATCCGAGTGATGAATCCCATTCAAGGGAACACACTGGACCTTGTC; CAGCTGGAACAGAATGAGGCAGCTTTTAGTGTGGCTGTGTGCAGGTTTTCCAACACTGGT; GAAGACTGGTATGTGCTGGTGGGTGTGGCCAAGGACCTGATACTAAACCCCCGATCTGTG; GCAGGGGGCTTCGTCTATACTTACAAGCTTGTGAACAATGGGGAAAAACTGGAGTTTTTG; CACAAGACTCCTGTGGAAGAGGTCCCTGCTGCTATTGCCCCATTCCAGGGGAGGGTGTTG; ATTGGTGTGGGGAAGCTGTTGCGTGTCTATGACCTGGGAAAGAAGAAGTTACTCCGAAAA; TGTGAGAATAAGCATATTGCCAATTATATCTCTGGGATCCAGACTATTGGACATAGGGTA; ATTGTATCTGATGTCCAAGAAAGTTTCATCTGGGTTCGCTACAAGCGTAATGAAAACCAG; CTTATCATCTTTGCTGATGATACCTACCCCCGATGGGTCACTACAGCCAGCCTCCTGGAC; TATGACACTGTGGCTGGGGCAGACAAGTTTGGCAACATATGTGTGGTGAGGCTCCCACCT; AACACCAATGATGAAGTAGATGAGGATCCTACAGGAAACAAAGCCCTGTGGGACCGTGGC; TTGCTCAATGGGGCCTCCCAGAAGGCAGAGGTGATCATGAACTACCATGTCGGGGAGACG; GTGCTGTCCTTGCAGAAGACCACGCTGATCCCTGGAGGCTCAGAATCACTTGTCTATACC; ACCTTGTCTGGAGGAATTGGCATCCTTGTGCCATTCACGTCCCATGAGGACCATGACTTC; TTCCAGCATGTGGAAATGCACCTGCGGTCTGAACATCCCCCTCTCTGTGGGCGGGACCAC; CTCAGCTTTCGCTCCTACTACTTCCCTGTGAAGAATGTGATTGATGGAGACCTCTGTGAG; CAGTTCAATTCCATGGAACCCAACAAACAAAAGAACGTCTCTGAAGAACTGGACCGAACC; CCACCCGAAGTGTCCAAGAAACTCGAGGATATCCGGACCCGCTACGCCTTCTGA; ; Clone variation with respect to NM_012426.4; 717 a=>t;2988 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012426 unedited; GCGAATTCGGCACGAGGTCTAATGGCGGACGCCAGTATGTTGGAGTTGGTGGTGGCTTAA; GTTTTGAAGGGAGGTAGCATCCGTTGGATATCCACACCATCCTTCTCGCTGCAGGCTTTC; TTGGACTCCGTACTGTTGGTGTAACCAAGGCCTGGAGGTCTGGGTGGCTCAGGTTTCCTG; CAGCCATGTTTCTGTACAACTTAACCTTGCAGAGAGCCACTGGCATCAGCTTTGCCATTC; ATGGAAACTTTTCTGGAACCAAACAACAAGAAATTGTTGTTTCCCGTGGGAAGATCTTGG; AGCTGCTTCGCCCAGACCCCAACACTGGCAAAGTACATACCCTACTCACTGTGGAAGTAT; TCGGTGTTATCCGGTCACTCATGGCCTTTAGGCTGACAGGTGGCACCAAAGACTACATTG; TAGTTGGCAGTGACTCTGGTCGAATTGTTATTTTGGAATACCAGCCATCTAAGAATATGT; TTGAGAAGATTCACCAAGAAACCTTTGGCAAGAGTGGATGCCGTCGCATCGTTCCTGGCC; AGTTCTTAGCTGTGGATCCCAAAGGGCGAGCCGTTATGATTAGTGCCATTGAGAAACAGA; AATTGGTGTATATTTTGAACAGAGATGCTGCAGCCCGACTTACCATTTCATCTCCCCTGG; AAGCCCACAAAGCANACACTTTAGTGTATCATGTAGTTGGAGTAGATGTCGGATTTGAAA; TCCAATGTTTGCTTGTCTGGA;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_012426
• Insert Size: 4430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012426.3, NP_036558.3
• RefSeq Size: 4357 bp
• RefSeq ORF: 3654 bp
• Locus ID: 23450
• UniProt ID: Q15393
• Cytogenetics: 16q22.1
• Domains: CPSF_A
• Protein Pathways: Spliceosome
• Summary: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]