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RAD18 (NM_020165) Human Untagged Clone

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SKU
SC323786
RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RAD18
Synonyms RNF73
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_020165.2 GTGTTGGAGCGCCGGAGGCTAGTGGGTGGCTGACCCCCAGCATCCTCGAGAGCGACCATG
GACTCCCTGGCCGAGTCTCGGTGGCCTCCGGGCCTGGCAGTCATGAAGACAATAGATGAT
TTGCTGCGGTGTGGAATTTGCTTCGAGTATTTCAACATTGCAATGATAATACCTCAGTGT
TCACATAACTACTGCTCTCTCTGTATAAGAAAATTTCTGTCCTATAAAACTCAGTGTCCA
ACTTGCTGTGTGACTGTCACAGAGCCGGATCTGAAAAATAACCGCATATTAGATGAACTG
GTAAAAAGCTTGAATTTTGCACGGAATCATCTGCTGCAGTTTGCTTTAGAGTCACCAGCC
AAATCTCCTGCTTCTTCCTCTTCAAAGAATCTTGCTGTCAAAGTATATACTCCTGTAGCC
TCCAGACAGTCTTTAAAGCAGGGGAGCAGGTTAATGGATAATTTCTTGATCAGAGAAATG
AGTGGTTCTACATCAGAGTTGTTGATAAAAGAAAATAAAAGCAAATTCAGCCCTCAAAAA
GAGGCGAGCCCTGCTGCAAAGACCAAAGAGACACGTTCTGTAGAAGAGATCGCTCCAGAT
CCCTCAGAGGCTAAGCGTCCTGAGCCACCCTCGACATCCACTTTGAAACAAGTTACTAAA
GTGGATTGTCCTGTTTGCGGGGTTAACATTCCAGAAAGTCACATTAATAAGCATTTAGAC
AGCTGTTTATCACGCGAAGAGAAGAAGGAAAGCCTCAGAAGTTCTGTTCACAAAAGGAAG
CCGCTGCCCAAAACTGTATATAATTTGCTCTCTGATCGTGATTTAAAGAAAAAGCTAAAA
GAGCATGGATTATCTATTCAAGGAAATAAACAACAGCTCATTAAAAGGCACCAAGAATTT
GTACACATGTACAATGCCCAATGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATAGTT
CAAGAAATCGAAAATATAGAGAAGACTAGGATGCGTCTTGAAGCTAGTAAACTCAATGAA
AGTGTAATGGTTTTTACAAAGGACCAAACAGAAAAGGAAATAGATGAAATCCACAGTAAA
TATCGTAAAAAACATAAGAGTGAATTTCAGCTTCTGGTGGATCAGGCTAGAAAAGGATAC
AAGAAAATTGCTGGAATGTCACAAAAAACAGTAACAATAACAAAAGAAGATGAATCTACA
GAAAAGCTATCTTCTGTATGCATGGGACAGGAAGATAATATGACCTCAGTAACAAACCAC
TTTTCTCAATCAAAGCTGGACTCCCCAGAGGAATTGGAACCTGACAGAGAAGAGGATTCT
TCTAGCTGTATTGATATTCAAGAAGTTCTTTCTTCATCAGAATCAGATTCATGCAATAGT
TCCAGTTCAGACATCATAAGAGATCTTTTAGAAGAAGAGGAAGCCTGGGAAGCATCACAT
AAAAACGATCTTCAAGACACAGAAATAAGTCCAAGACAGAATCGCCGCACAAGAGCCGCT
GAAAGTGCTGAGATTGAACCAAGAAACAAGCGTAATAGGAATTAATGTGGGCTTTTGCTG
ACTTTTCAAATGCATTGATTAGAATACCGTACTTTTGGTTGCCACAGATAGATTTTCTAT
TTATAAATGCCCAAGGAAAGATGCTAAATTCTAAATATTACGGTTAGCTGATATTCATTC
TTTTCTGCTTTTCCAGAGGGGAAAAATGTTACAAAATATCCTTACTTGGTCAGTTGCCTC
CTGCCTCTAAAACATCTCTCTCTAAAAATACTGACATTTCACACAGGTACCAGCTTTGCA
GAGGAGGTAGACTCTTTGGCACTTTGGCACAGGGATTTGGTTTGGTTTGGTTTGGTTTGA
TAATTAAATTTCAGAATGTCAAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_020165
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020165.2, NP_064550.2
RefSeq Size 3053 bp
RefSeq ORF 1488 bp
Locus ID 56852
UniProt ID Q9NS91
Cytogenetics 3p25.3
Domains RING, SAP, ZnF_Rad18
Protein Families Druggable Genome
Summary The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_020165" in other vectors (7)
SKU Description Size Price
RC201700 RAD18 (Myc-DDK-tagged)-Human RAD18 homolog (S. cerevisiae) (RAD18) 10 ug
$457.00
RC201700L1 Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), Myc-DDK-tagged 10 ug
$757.00
RC201700L2 Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), mGFP tagged 10 ug
$757.00
RC201700L3 Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), Myc-DDK-tagged 10 ug
$757.00
RC201700L4 Lenti ORF clone of Human RAD18 homolog (S. cerevisiae) (RAD18), mGFP tagged 10 ug
$757.00
RG201700 RAD18 (tGFP-tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18) 10 ug
$489.00 $657.00
SC108413 RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18) 10 ug
$457.00

file-alt Citations

Evidence for Ubiquitin-Regulated Nuclear and Subnuclear Trafficking among Paramyxovirinae Matrix Proteins
Pentecost, Vashisht, Lester et al
PLoS Pathog. (2015) 11 (3), e1004739 DOI: 10.1371/journal.ppat.1004739
Cited by 90
AI Summary: OriGene contributed to this study by providing the RAD18 cDNA clone (catalog number SC323786), which was used to generate 3X-Flag-GFP-RAD18 constructs for investigating the ubiquitin-regulated nuclear trafficking of paramyxovirus matrix proteins.
Snippet: …A RAD18 cDNA clone was purchased from Origene (SC323786). 3X-Flag-GFP-RAD18 was generated by replacement of the NiV-M insert in HindIII/XhoI digested 3X-Flag-NiV-M
FigureFigureFigure
Showing 1-1 of 1 papers.
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