TAB1 (NM_006116) Human Untagged Clone

SKU SC322672

TAB1 (untagged)-Human TGF-beta activated kinase 1/MAP3K7 binding protein 1 (TAB1), transcript variant alpha

Product Data

• Type: Human Untagged Clone
• Target Symbol: TAB1
• Synonyms: 3'-Tab1; MAP3K7IP1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322672 CTCTAGCCGCCCCACTCGCTGCTCTGCGGGGAGGCGGGCGCTCCCGCAGGGGTTCCTCCA; AGATGGCGGCGCAGAGGAGGAGCTTGCTGCAGAGTGAGCAGCAGCCAAGCTGGACAGATG; ACCTGCCTCTCTGCCACCTCTCTGGGGTTGGCTCAGCCTCCAACCGCAGCTACTCTGCTG; ATGGCAAGGGCACTGAGAGCCACCCGCCAGAGGACAGCTGGCTCAAGTTCAGGAGTGAGA; ACAACTGCTTCCTGTATGGGGTCTTCAACGGCTATGATGGCAACCGAGTGACCAACTTCG; TGGCCCAGCGGCTGTCCGCAGAGCTCCTGCTGGGCCAGCTGAATGCCGAGCACGCCGAGG; CCGATGTGCGGCGTGTGCTGCTGCAGGCCTTCGATGTGGTGGAGAGGAGCTTCCTGGAGT; CCATTGACGACGCCTTGGCTGAGAAGGCAAGCCTCCAGTCGCAATTGCCAGAGGGAGTCC; CTCAGCACCAGCTGCCTCCTCAGTATCAGAAGATCCTTGAGAGACTCAAGACGTTAGAGA; GGGAAATTTCGGGAGGGGCCATGGCCGTTGTGGCGGTCCTTCTCAACAACAAGCTCTACG; TCGCCAATGTCGGTACAAACCGTGCACTTTTATGCAAATCGACAGTGGATGGGTTGCAGG; TGACACAGCTGAACGTGGACCACACCACAGAGAACGAGGATGAGCTCTTCCGTCTTTCGC; AGCTGGGCTTGGATGCTGGAAAGATCAAGCAGGTGGGGATCATCTGTGGGCAGGAGAGCA; CCCGGCGGATCGGGGATTACAAGGTTAAATATGGCTACACGGACATTGACCTTCTCAGCG; CTGCCAAGTCCAAACCAATCATCGCAGAGCCAGAAATCCATGGGGCACAGCCGCTGGATG; GGGTGACGGGCTTCTTGGTGCTGATGTCGGAGGGGTTGTACAAGGCCCTAGAGGCAGCCC; ATGGGCCTGGGCAGGCCAACCAGGAGATTGCTGCGATGATTGACACTGAGTTTGCCAAGC; AGACCTCCCTGGACGCAGTGGCCCAGGCCGTCGTGGACCGGGTGAAGCGCATCCACAGCG; ACACCTTCGCCAGTGGTGGGGAGCGTGCCAGGTTCTGCCCCCGGCACGAGGACATGACCC; TGCTAGTGAGGAACTTTGGCTACCCGCTGGGCGAAATGAGCCAGCCCACACCGAGCCCAG; CCCCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTGTGCCATACTCCAGCGCCCAGAGCA; CCAGCAAGACCAGCGTGACCCTCTCCCTTGTCATGCCCTCCCAGGGCCAGATGGTCAACG; GGGCTCACAGTGCTTCCACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAAGCCCGA; CCTTAACCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTGACGGAG; GCCTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGACGGTCGTGTTGAGC; CCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGAGCGTGGACCATGGCGAGCAGAGCG; TGGTGACAGCACCGTAGGGCAGCCGGAGAATGCAGCCCAAGCAGGGCCTGGCATGGGGCA; GGACAGGGTCCAGCCTTTTCCTAACATCTGCCTGTGCCACAACGGCCAGCAGGTGCCCCA; TCCTCTGCCCACAGCAGACTCTGTCCCATGGCTCTCCGGGCAGTAGAGTGTGTGAGTGCA; GACTGGACCTGTGGTTCATACCTTGTCACCACCCGGGAAGCTGAAGGCCACTTCCTCCCA; GATGGCCTCAGCCAGGACCATCGCCCTTTCTCAGAGCAGAGGGCCAGGTATAGAAACCGC; AGTGGGCCTGCAAGCCGCCCGAGCCTCCCCAGCAGCCTCCTACAGAGCAGGAAGAGGCGC; CCTGTGAACCCTGAGTGTTGCAGGCCCAGCAGACCCTGCTGTCCCAAGCCCACCCCTCCT; CCCACCATCACCTCCCTCACCTCGGGACAGTAGCCCTCCACTTCTCCAGCCTCTCAGCCC; TGTGCTCCTGCATCCAGAGTGGAACCCAGGCTGGTGTCCGCATCTGTCCCTGGGCCCCAC; CCCTGGACCTGCCTTGGTTGTGTCATCTGTTGTAAACGTTCAGGAGGACCAGGGCAGCAT; CTGGGGCCTGGGATGGCCACAGAAGGGGCAGGCCAGGTGGAAAGGAGCCAGGGGGAAGTG; GTCTAAGAGACCTGGAACTGCCAGAGGATGGCGGCCTGGGCTTCCCCAGAGCCAGGCGTG; CGGGAGAGGTGAGGACTGGCCCCGGTGGGCTGAGGCAGGGGCCGCTGTCGTCAGGCCTGA; GCCAGGGTGAGCTGGTGCCTGCCTTGCATTTTCCTTCTGGTGCTGTGAAGACCATAGGCT; GGCAGGCAGCTGAGATGAACTGTCTTTACCACTGATGAGGGGCCTCTGCCGGCTGAGGGT; AGCAAGCAGGGGTTGTGAGTCAGGCTGGGGGACTTGTTTGAAAGAAAGAGGAGTGGAAAA; TGGTTCCAGGAGGGAAGAGGTTCTTTGAGACACAGTACCCTGGGAGGCATAGGAGAAGGG; TCGGGCCAGCCCAGCCCAGGGCCTGAGTTAGACTATTTCCCACATGTTCTCTGCCTTCAG; TGGGGAGGGGGTGCCACCAGGGCTGTCGGCCAGGATTGCCACTCCTGTTTCAGAGGAAGC; AGGCCGAGAGACTTGCACCTTGGCCAAGCCACACAATCAGTGGGGCAGCCAGAGCTCAGA; CCTGAGCCATTTTGTCAGTATCCAGGACCCCCCGGATTCTCCACGCCCTCCCCATCTCCC; AGTCTCCCTGCCCCCCATGCCCCAGACCGGCCCACCAGGGACTAGCCGCTGTCGCACAGC; CTCTGGGGTGCTTGGTCTCTGCGAAGTCAAAGGCCTGACAGCTCTGTGGCCTGGGAATCC; ATTTTCCTGCGGCAGAGCAGGGCCTGGTGTGGAACCAGGGAGCTGTGGGAAGCCACAGCA; GAAATGGAAGAAAAACAGGTCTCAGCCCAGGGTCCTCGCTCACTCCCTCACTCCCCACTT; TGAAGCCATCTCTGTTCTGCAGGTGAGAGGATTTAAAGTCAGTCACAAAGGCTTGGGAAC; AAAAGGAATTCTCTTCCAAGAATGCCTCTGTGGTGCTGTTTGGTCTCTAGAAACAGGGTC; ACTTTTTTAATGTAGTAAAGAAGTAATAAATGGTGGCATTACACATTGTGATATTAAAAA; AAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006116
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006116.2, NP_006107.1
• RefSeq Size: 3240 bp
• RefSeq ORF: 1515 bp
• Locus ID: 10454
• UniProt ID: Q15750
• Cytogenetics: 22q13.1
• Domains: PP2C
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, NOD-like receptor signaling pathway, Toll-like receptor signaling pathway
• Summary: The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (alpha) encodes the longer isoform (alpha).