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MID2 (NM_052817) Human Untagged Clone

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SKU
SC322490
MID2 (untagged)-Human midline 2 (MID2), transcript variant 2
  $628.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MID2
Synonyms FXY2; MRX101; RNF60; TRIM1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for SC322490 GCCTCAGGAGGACTATTTTCACTAAAGATGGAAACACTGGAGTCTGAATTGACCTGTCCA
ATCTGCCTAGAGTTGTTTGAAGACCCCCTTCTGCTCCCTTGTGCTCACAGCCTCTGCTTC
AGCTGTGCCCATCGCATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAACCCATT
ACTGCTTTCCAGTGTCCTACCTGCAGGTATGTTATCTCGCTGAACCACCGGGGCCTGGAT
GGCCTCAAGAGGAATGTGACTCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTC
AGTGGGCCCAATTCCCCTAGTGAGAGCCGCCGGGAAAGGACTTACAGGCCCACCACTGCC
ATGTCTAGCGAGCGAATTGCTTGCCAATTCTGTGAGCAGGACCCGCCAAGGGATGCAGTA
AAAACATGCATCACCTGTGAGGTCTCCTACTGTGACCGTTGCCTGCGGGCCACGCACCCC
AACAAGAAACCTTTCACCAGCCACCGCCTGGTGGAACCAGTGCCAGACACACATCTTCGA
GGGATCACCTGCCTGGACCATGAGAATGAGAAAGTGAACATGTACTGTGTATCTGATGAC
CAATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCATCC
CTGAATGATCGATTTGAGAAACTCAAGCAAACTCTGGAGATGAACCTCACCAACCTGGTT
AAGCGCAACAGCGAACTAGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAGGTT
GAGGTGAATACTGCTATGCATGAGGCAAAACTTATGGAAGAATGTGACGAGTTGGTAGAG
ATCATCCAGCAGAGGAAGCAAATGATCGCTGTCAAAATCAAAGAGACAAAGGTTATGAAA
CTGAGAAAGTTGGCACAGCAGGTTGCTAATTGCCGCCAGTGTCTTGAACGGTCAACAGTC
CTCATCAACCAAGCTGAGCATATCCTGAAAGAAAATGACCAGGCACGGTTTCTACAGTCT
GCAAAAAATATTGCTGAGAGGGTCGCTATGGCAACTGCATCTTCTCAAGTTCTGATTCCA
GACATCAATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCCAGAGAAAAGAAA
CTGCTAGAGGGGTTAGATTATTTAACAGCCCCAAACCCACCATCTATCCGAGAAGAACTC
TGTACTGCCTCCCATGACACCATTACAGTCCACTGGATCTCGGATGATGAGTTCAGCATC
AGCTCCTATGAGCTTCAGTACACCATATTCACTGGCCAGGCTAACTTCATCAGCCTGTAT
AATTCAGTAGACAGCTGGATGATTGTTCCCAACATTAAACAGAACCATTACACAGTGCAT
GGACTCCAGAGCGGGACTCGCTACATCTTCATCGTTAAAGCCATAAACCAAGCCGGCAGC
CGGAACAGTGAACCTACCCGACTAAAAACAAACAGCCAACCCTTTAAATTGGATCCCAAA
ATGACTCACAAGAAGTTGAAGATCTCCAATGATGGATTGCAGATGGAGAAGGATGAAAGC
TCTCTAAAGAAGAGCCACACCCCAGAGAGGTTTAGTGGCACAGGGTGCTATGGGGCAGCA
GGAAATATATTCATTGACAGTGGCTGCCACTATTGGGAGGTGGTCATGGGTTCCTCAACA
TGGTATGCAATTGGCATTGCCTACAAATCAGCTCCAAAGAATGAATGGATTGGCAAGAAT
GCCTCCTCATGGGTCTTCTCTCGCTGCAATAGTAACTTCGTGGTGAGACACAACAACAAG
GAAATGCTGGTGGATGTGCCCCCACACCTGAAGCGTCTGGGTGTCCTCCTGGATTATGAC
AACAATATGCTGTCTTTCTATGACCCAGCTAACTCTCTCCATCTTCATACTTTTGATGTG
ACCTTCATTCTTCCAGTTTGTCCAACATTTACAATCTGGAACAAATCCCTAATGATCCTG
TCTGGCTTGCCTGCCCCAGATTTTATTGATTACCCTGAGCGGCAGGAATGCAACTGCAGG
CCTCAAGAATCCCCTTATGTTTCTGGGATGAAAACCTGTCATTAAGTTTCAGGAGAGTAT
ATAATTCACTGGCTCTCTAGTTCAGCAGTTCTTCCCCTCCTACACCTAAGTTAGCGTTCA
ATATACGAGACACAAAATAAAGTTTGTTTGAAGCATCCAAAATAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_052817
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052817.1, NP_438112.1
RefSeq Size 7255 bp
RefSeq ORF 2058 bp
Locus ID 11043
UniProt ID Q9UJV3
Cytogenetics Xq22.3
Domains BBC, FN3, RING, SPRY, zf-B_box
Protein Families Druggable Genome
Summary The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. provided by RefSeq, Feb 2009
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_052817" in other vectors (6)
SKU Description Size Price
RC204429 MID2 (Myc-DDK-tagged)-Human midline 2 (MID2), transcript variant 2 10 ug
$627.00
RC204429L3 Lenti ORF clone of Human midline 2 (MID2), transcript variant 2, Myc-DDK-tagged 10 ug
$927.00
RC204429L4 Lenti ORF clone of Human midline 2 (MID2), transcript variant 2, mGFP tagged 10 ug
$927.00
RG204429 MID2 (tGFP-tagged) - Human midline 2 (MID2), transcript variant 2 10 ug
$827.00
SC110211 MID2 (untagged)-Human midline 2 (MID2), transcript variant 2 10 ug
$628.00
SC327978 MID2 (untagged)-Human midline 2 (MID2) transcript variant 2 10 ug
$711.00

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