ASCC1 (NM_015947) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | ASCC1 |
| Synonyms | ASC1p50; CGI-18; RP11-150D20.4 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_015947 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015947.2, NP_057031.2 |
| RefSeq Size | 2090 bp |
| RefSeq ORF | 1074 bp |
| Locus ID | 51008 |
| Cytogenetics | 10q22.1 |
| Domains | KH |
| Summary | This gene encodes a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF). The encoded protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Mutations in this gene are associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. provided by RefSeq, Dec 2011 Transcript Variant: This variant (3) differs in both UTRs and has multiple coding region differences compared to variant 1, one of which results in a frameshift. The resulting isoform (b) has a distinct C-terminus and is shorter than isoform a. Variants 2, 3, and 4 all encode the same isoform (b). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_015947" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201872 | ASCC1 (Myc-DDK-tagged)-Human activating signal cointegrator 1 complex subunit 1 (ASCC1), transcript variant 3 | 10 ug |
$457.00
|
|
| RC201872L3 | Lenti ORF clone of Human activating signal cointegrator 1 complex subunit 1 (ASCC1), transcript variant 3, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC201872L4 | Lenti ORF clone of Human activating signal cointegrator 1 complex subunit 1 (ASCC1), transcript variant 3, mGFP tagged | 10 ug |
$757.00
|
|
| RG201872 | ASCC1 (tGFP-tagged) - Human activating signal cointegrator 1 complex subunit 1 (ASCC1), transcript variant 3 | 10 ug |
$657.00
|
|
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