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Lipoprotein lipase (LPL) (NM_000237) Human Untagged Clone

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SKU
SC322258
LPL (untagged)-Human lipoprotein lipase (LPL)
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Lipoprotein lipase
Synonyms HDLCQ11; LIPD
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for SC322258 GCTCAGCCGGCTCATCAGTCGGTCCGCGCCTTGCAGCTCCTCCAGAGGGACGCGCCCCGA
GATGGAGAGCAAAGCCCTGCTCGTGCTGACTCTGGCCGTGTGGCTCCAGAGTCTGACCGC
CTCCCGCGGAGGGGTGGCCGCCGCCGACCAAAGAAGAGATTTTATCGACATCGAAAGTAA
ATTTGCCCTAAGGACCCCTGAAGACACAGCTGAGGACACTTGCCACCTCATTCCCGGAGT
AGCAGAGTCCGTGGCTACCTGTCATTTCAATCACAGCAGCAAAACCTTCATGGTGATCCA
TGGCTGGACGGTAACAGGAATGTATGAGAGTTGGGTGCCAAAACTTGTGGCCGCCCTGTA
CAAGAGAGAACCAGACTCCAATGTCATTGTGGTGGACTGGCTGTCACGGGCTCAGGAGCA
TTACCCAGTGTCCGCGGGCTACACCAAACTGGTGGGACAGGATGTGGCCCGGTTTATCAA
CTGGATGGAGGAGGAGTTTAACTACCCTCTGGACAATGTCCATCTCTTGGGATACAGCCT
TGGAGCCCATGCTGCTGGCATTGCAGGAAGTCTGACCAATAAGAAAGTCAACAGAATTAC
TGGCCTCGATCCAGCTGGACCTAACTTTGAGTATGCAGAAGCCCCGAGTCGTCTTTCTCC
TGATGATGCAGATTTTGTAGACGTCTTACACACATTCACCAGAGGGTCCCCTGGTCGAAG
CATTGGAATCCAGAAACCAGTTGGGCATGTTGACATTTACCCGAATGGAGGTACTTTTCA
GCCAGGATGTAACATTGGAGAAGCTATCCGCGTGATTGCAGAGAGAGGACTTGGAGATGT
GGACCAGCTAGTGAAGTGCTCCCACGAGCGCTCCATTCATCTCTTCATCGACTCTCTGTT
GAATGAAGAAAATCCAAGTAAGGCCTACAGGTGCAGTTCCAAGGAAGCCTTTGAGAAAGG
GCTCTGCTTGAGTTGTAGAAAGAACCGCTGCAACAATCTGGGCTATGAGATCAGTAAAGT
CAGAGCCAAAAGAAGCAGCAAAATGTACCTGAAGACTCGTTCTCAGATGCCCTACAAAGT
CTTCCATTACCAAGTAAAGATTCATTTTTCTGGGACTGAGAGTGAAACCCATACCAATCA
GGCCTTTGAGATTTCTCTGTATGGCACCGTGGCCGAGAGTGAGAACATCCCATTCACTCT
GCCTGAAGTTTCCACAAATAAGACCTACTCCTTCCTAATTTACACAGAGGTAGATATTGG
AGAACTACTCATGTTGAAGCTCAAATGGAAGAGTGATTCATACTTTAGCTGGTCAGACTG
GTGGAGCAGTCCCGGCTTCGCCATTCAGAAGATCAGAGTAAAAGCAGGAGAGACTCAGAA
AAAGGTGATCTTCTGTTCTAGGGAGAAAGTGTCTCATTTGCAGAAAGGAAAGGCACCTGC
GGTATTTGTGAAATGCCATGACAAGTCTCTGAATAAGAAGTCAGGCTGAAACTGGGCGAA
TCTACAGAACAAAGAACGGCATGTGAATTCTGTGAAGAATGAAGTGGAGGAAGTAACTTT
TACAAAACATACCCAGTGTTTGGGGTGTTTCAAAAGTGGATTTTCCTGAATATTAATCCC
AGCCCTACCCTTGTTAGTTATTTTAGGAGACAGTCTCAAGCACTAAAAAGTGGCTAATTC
AATTTATGGGGTATAGTGGCCAAATAGCACATCCTCCAACGTTAAAAGACAGTGGATCAT
GAAAAGTGCTGTTTTGTCCTTTGAGAAAGAAATAATTGTTTGAGCGCAGAGTAAAATAAG
GCTCCTTCATGTGGCGTATTGGGCCATAGCCTATAATTGGTTAGAACCTCCTATTTTAAT
TGGAATTCTGGATCTTTCGGACTGAGGCCTTCTCAAACTTTACTCTAAGTCTCCAAGAAT
ACAGAAAATGCTTTTCCGCGGCACGAATCAGACTCATCTACACAGCAGTATGAATGATGT
TTTAGAATGATTCCCTCTTGCTATTGGAATGTGGTCCAGACGTCAACCAGGAACATGTAA
CTTGGAGAGGGACGAAGAAAGGGTCTGATAAACACAGAGGTTTTAAACAGTCCCTACCAT
TGGCCTGCATCATGACAAAGTTACAAATTCAAGGAGATATAAAATCTAGATCAATTAATT
CTTAATAGGCTTTATCGTTTATTGCTTAATCCCTCTCTCCCCCTTCTTTTTTGTCTCAAG
ATTATATTATAATAATGTTCTCTGGGTAGGTGTTGAAAATGAGCCTGTAATCCTCAGCTG
ACACATAATTTGAATGGTGCAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_000237
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000237.1, NP_000228.1
RefSeq Size 3549 bp
RefSeq ORF 1428 bp
Locus ID 4023
UniProt ID P06858
Cytogenetics 8p21.3
Domains lipase, PLAT
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Glycerolipid metabolism, PPAR signaling pathway
Summary LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000237" in other vectors (7)
SKU Description Size Price
RC203766 LPL (Myc-DDK-tagged)-Human lipoprotein lipase (LPL) 10 ug
$289.00 $686.00
RC203766L1 Lenti ORF clone of Human lipoprotein lipase (LPL), Myc-DDK-tagged 10 ug
$986.00
RC203766L2 Lenti ORF clone of Human lipoprotein lipase (LPL), mGFP tagged 10 ug
$986.00
RC203766L3 Lenti ORF clone of Human lipoprotein lipase (LPL), Myc-DDK-tagged 10 ug
$986.00
RC203766L4 Lenti ORF clone of Human lipoprotein lipase (LPL), mGFP tagged 10 ug
$986.00
RG203766 LPL (tGFP-tagged) - Human lipoprotein lipase (LPL) 10 ug
$489.00 $886.00
SC120026 LPL (untagged)-Human lipoprotein lipase (LPL) 10 ug
$457.00

file-alt Citations

Endocytosis of very low-density lipoproteins: an unexpected mechanism for lipid acquisition by breast cancer cells
Lupien, Bloch, Dehairs et al
J. Lipid Res. (2019)
AI Summary: OriGene products contributed to this study by providing the HuSH shRNA plasmid panels with pGFP-C-Lenti vectors, the Lenti-vpack Packaging Kit (TR30037), and the pCMV-6AC vector for human LPL expression, which were used to manipulate gene expression and investigate lipid uptake mechanisms in breast cancer cells.
Snippet: using HuSH shRNA plasmid panels with pGFP-C-Lenti vectors and the Lenti-vpack Packaging Kit (TR30037) according to manufacturer guidelines (OriGene Technologies, Rockville, MD) Human LPL was expressed from a pCMV-6AC vector from OriGene (Rockville
Endocytosis of very low-density lipoprotein particles: an unexpected mechanism for lipid acquisition by breast cancer cells
Lupien, Bloch, Dehairs et al
bioRxiv (2019)
Snippet: MDA-MB-231 shRNA cells were generated through lentiviral transduction using HuSH shRNA plasmid panels (29 mer) with pGFP-C-Lenti vectors and the Lenti- vpack Packaging Kit (TR30037) according to manufacturer guidelines (OriGene Technologies, Rockville View as HTML
Showing 1-2 of 2 papers.
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