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CD247 (NM_000734) Human Untagged Clone

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SKU
SC321778
CD247 (untagged)-Human CD247 molecule (CD247), transcript variant 2
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CD247
Synonyms CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000734.2 CGTCCCGGCCACCGCTGCCTCAGCCTCTGCCTCCCAGCCTCTTTCTGAGGGAAAGGACAA
GATGAAGTGGAAGGCGCTTTTCACCGCGGCCATCCTGCAGGCACAGTTGCCGATTACAGA
GGCACAGAGCTTTGGCCTGCTGGATCCCAAACTCTGCTACCTGCTGGATGGAATCCTCTT
CATCTATGGTGTCATTCTCACTGCCTTGTTCCTGAGAGTGAAGTTCAGCAGGAGCGCAGA
CGCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCTCAATCTAGGACGAAG
AGAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGGGGGAAAGCC
GCAGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGATAAGATGGC
GGAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGGGGCACGATGG
CCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCTTCACATGCAGGC
CCTGCCCCCTCGCTAACAGCCAGGGGATTTCACCACTCAAAGGCCAGACCTGCAGACGCC
CAGATTATGAGACACAGGATGAAGCATTTACAACCCGGTTCACTCTTCTCAACCACTGAA
GTATTCCCCTTTATGTACAGGATGCTTTGGTTATATTTAGCTCCAAACCTTCACACACAG
ACTGTTGTCCCTGCACTCTTTAAGGGAGTGTACTCCCAGGGCTTACGGCCCTGGCCTTGG
GCCCTCTGGTTTGCCGGTGGTGCAGGTAGACCTGTCTCCTGGCGGTTCCTCGTTCTCCCT
GGGAGGCGGGCGCACTGCCTCTCACAGCTGAGTTGTTGAGTCTGTTTTGTAAAGTCCCCA
GAGAAAGCGCAGATGCTAGCACATGCCCTAATGTCTGTATCACTCTGTGTCTGAGTGGCT
TCACTCCTGCTGTAAATTTGGCTTCTGTTGTCACCTTCACCTCCTTTCAAGGTAACTGTA
CTGGGCCATGTTGTGCCTCCCTGGTGAGAGGGCCGGGCAGAGGGGCAGATGGAAAGGAGC
CTAGGCCAGGTGCAACCAGGGAGCTGCAGGGGCATGGGAAGGTGGGCGGGCAGGGGAGGG
TCAGCCAGGGCCTGCGAGGGCAGCGGGAGCCTCCCTGCCTCAGGCCTCTGTGCCGCACCA
TTGAACTGTACCATGTGCTACAGGGGCCAGAAGATGAACAGACTGACCTTGATGAGCTGT
GCACAAAGTGGCATAAAAAACATGTGGTTACACAGTGTGAATAAAGTGCTGCGGAGCAAG
AGGAGGCCGTTGATTCACTTCACGCTTTCAGCGAATGACAAAATCATCTTTGTGAAGGCC
TCGCAGGAAGACCCAACACTTGGGACCTATAACTGCCCAGCGGACAGTGGCAGGACAGGA
AAAACCCGTCAATGTACTAGGATACTGCTGCGTCATTACAGGGCACAGGCCATGGATGGA
AAACGCTCTCTGCTCTGCTTTTTTTCTACTGTTTTAATTTATACTGGCATGCTAAAGCCT
TCCTATTTTGCATAATAAATGCTTCAGTGAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_000734
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000734.2, NP_000725.1
RefSeq Size 1674 bp
RefSeq ORF 492 bp
Locus ID 919
UniProt ID P20963
Cytogenetics 1q24.2
Domains ITAM
Protein Families Druggable Genome, Transmembrane
Protein Pathways Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway
Summary The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) uses an alternate splice site in the mid-coding region, which results in a missing in-frame codon, as compared to variant 1. The encoded isoform (2) is thus missing an internal amino acid, as compared to isoform 1.
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"NM_000734" in other vectors (6)
SKU Description Size Price
RC206562 CD247 (Myc-DDK-tagged)-Human CD247 molecule (CD247), transcript variant 2 10 ug
$150.00
RC206562L1 Lenti ORF clone of Human CD247 molecule (CD247), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC206562L2 Lenti ORF clone of Human CD247 molecule (CD247), transcript variant 2, mGFP tagged 10 ug
$450.00
RC206562L3 Lenti ORF clone of Human CD247 molecule (CD247), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC206562L4 Lenti ORF clone of Human CD247 molecule (CD247), transcript variant 2, mGFP tagged 10 ug
$450.00
RG206562 CD247 (tGFP-tagged) - Human CD247 molecule (CD247), transcript variant 2 10 ug
$489.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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