PP2A-alpha (PPP2CA) (NM_002715) Human Untagged Clone
SKU
SC321401
PPP2CA (untagged)-Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA)
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PP2A-alpha |
| Synonyms | NEDLBA; PP2Ac; PP2CA; PP2Calpha; RP-C |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_002715 |
| Insert Size | 930 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002715.2, NP_002706.1 |
| RefSeq Size | 2643 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 5515 |
| UniProt ID | P67775 |
| Cytogenetics | 5q31.1 |
| Domains | Metallophos, PP2Ac |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| Protein Pathways | Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway |
| Summary | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. provided by RefSeq, Jul 2008 Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002715" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201334 | PPP2CA (Myc-DDK-tagged)-Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA) | 10 ug |
$300.00
|
|
| RC201334L1 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201334L2 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA), mGFP tagged | 10 ug |
$600.00
|
|
| RC201334L3 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC201334L4 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA), mGFP tagged | 10 ug |
$600.00
|
|
| RG201334 | PPP2CA (tGFP-tagged) - Human protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA) | 10 ug |
$489.00
$500.00
|
|
Citations
LASS2 enhances chemosensitivity to cisplatin by inhibiting PP2A-mediated β-catenin dephosphorylation in a subset of stem-like bladder cancer cells
Shi, Tan, Duan et al
BMC Med (2024) 22 (1), 19 DOI: 10.1186/s12916-023-03243-5
Shi, Tan, Duan et al
BMC Med (2024) 22 (1), 19 DOI: 10.1186/s12916-023-03243-5
Cited by 10
AI Summary: OriGene products contributed to this study by providing the LASS2 ORF expression plasmid (#RG215300) and the PP2A catalytic subunit expression plasmid (#SC321401), which were used to investigate the molecular mechanisms underlying cisplatin sensitivity in bladder cancer stem-like cells.
Snippet: rence efficiency (Additional file 2: Fig. S3E). The LASS2 ORF expression plasmid (#RG215300) and PP2A catalytic subunit expression plasmid (#SC321401) with the pCMV6-Ac-GFP vector were obtained from OriGene. siRNAs for GSK3β (sc-35525) and CK1α (sc-29912) were obtained from Santa Cruz. For some experiments, 5 × 104 cells were transfected with 0.1 μg/mL plasmid per well in 12-well dishes
Supervisor: Prof Paul Spiers
Konieczna
Thesis (2021)
Konieczna
Thesis (2021)
Snippet: Page 1. Role of Protein Phosphatase 2A inhibition in modulation of VE- cadherin and Claudin-5 in human brain microvascular endothelial cells and the potential reversal by small molecule activators of PP2A (SMAPs) A Thesis;The pCMV-6-PP2Acα expression plasmid (pcPP2Ac;cat. # SC321401) and pCMV6-AC (pCMV6;cat. # PS100020) were procured from Origene (Maryland, USA)
CD25 and Protein Phosphatase 2A Cooperate to Enhance IL-2R Signaling in Human Regulatory T Cells
Ding, Yu, Tsokos et al
J Immunol (2019) 203 (1), 93-104 DOI: 10.4049/jimmunol.1801570
Ding, Yu, Tsokos et al
J Immunol (2019) 203 (1), 93-104 DOI: 10.4049/jimmunol.1801570
Cited by 18
AI Summary: In this study, OriGene's pCMV6-Entry-PP2Ac plasmid and PP2Ac shRNA lentiviral particles were used to overexpress or knockdown PP2A catalytic subunit in human T cells, enabling the authors to demonstrate how PP2A modulates IL-2 receptor signaling and regulatory T cell function.
Involvement of vacuolar H(+) -ATPase in killing of human melanoma cells by the sphingosine kinase analogue FTY720
Tay, Liu, Chi et al
Pigment Cell Melanoma Res (2014)
Tay, Liu, Chi et al
Pigment Cell Melanoma Res (2014)
Cited by 21
AI Summary: OriGene's pCMV6-AC-PP2Ac plasmid was used in this study to investigate the role of protein phosphatase 2A in FTY720-induced cell death in human melanoma cells.
Snippet: Plasmid vectors and transfection The pCMV6-AC-PP2Ac construct was purchased from Origene (Rockville, MD, USA). Cells were transfected with 2 μg plasmid as well as the empty vector in Opti-MEM medium (Life Technologies) with Lipofectamine 2000 reagent (Life Technologies) according to the manufacturer's protocol
Suppression of PP2A is critical for protection of melanoma cells upon endoplasmic reticulum stress
Tay, Jin, Tseng et al
Cell Death Dis (2012) 3, e337 DOI: 10.1038/cddis.2012.79
Tay, Jin, Tseng et al
Cell Death Dis (2012) 3, e337 DOI: 10.1038/cddis.2012.79
Cited by 34
AI Summary: OriGene products pCMV6-XL4-Bim EL, pCMV6-AC-CHOP, and pCMV6-AC-PP2A-C plasmid vectors were used for gene overexpression experiments to investigate the roles of Bim, CHOP, and PP2A in melanoma cell responses to endoplasmic reticulum stress.
Snippet: RM.CHOP). Plasmid vectors and transfection. The pCMV6-XL4-Bim EL (SC306048), pCMV6-AC-CHOP (SC324377-20), and pCMV6-AC-PP2A-C (SC321401) were purchased from Origene (Rockville, MD, USA). Cells were
Showing 1-5 of 5 papers.
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