CAP1 (NM_006367) Human Untagged Clone
SKU
SC321219
CAP1 (untagged)-Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | CAP1 |
| Synonyms | CAP; CAP1-PEN |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_006367 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006367.2, NP_006358.1 |
| RefSeq Size | 2614 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 10487 |
| UniProt ID | Q01518 |
| Cytogenetics | 1p34.2 |
| Domains | CAP, CARP |
| Protein Families | Druggable Genome |
| Summary | The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Aug 2016 Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, and 4-10 encode the same isoform (a). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006367" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201394 | CAP1 (Myc-DDK-tagged)-Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1 | 10 ug |
$686.00
|
|
| RC201394L1 | Lenti ORF clone of Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC201394L2 | Lenti ORF clone of Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC201394L3 | Lenti ORF clone of Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC201394L4 | Lenti ORF clone of Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG201394 | CAP1 (tGFP-tagged) - Human CAP, adenylate cyclase-associated protein 1 (yeast) (CAP1), transcript variant 1 | 10 ug |
$886.00
|
|
Citations
Cyclase-associated protein (CAP) acts directly on F-actin to accelerate cofilin-mediated actin severing across the range of physiological pH
Normoyle, Brieher
J. Biol. Chem. (2012) 287 (42), 35722-32 DOI: 10.1074/jbc.M112.396051
Normoyle, Brieher
J. Biol. Chem. (2012) 287 (42), 35722-32 DOI: 10.1074/jbc.M112.396051
Cited by 9
AI Summary: OriGene contributed to this study by providing the human CAP1 cDNA, which was used for protein expression and functional analysis of CAP's role in actin filament severing.
Snippet: DNA Constructs. Human CAP1 cDNA was obtained from Origene and subcloned into the BamHI and SalI sites in the bacterial expression vector pET30a + (EMD) for expression in Rosetta cells (EMD) with an N-terminal His 6 tag. Purification of CAP from Bovine Thymus.
Cyclase associated protein (CAP) and the physiological disassembly of actin
Normoyle
Thesis (2015)
Normoyle
Thesis (2015)
Snippet: Human CAP1 cDNA was obtained from Origene and subcloned into the BamHI and SalI sites in the bacterial expression vector pET30a+ (EMD) for expression in Rosetta cells (EMD) with an N-terminal 6-His tag
Showing 1-2 of 2 papers.
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