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PHF1 (NM_024165) Human Untagged Clone

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SKU
SC320779
PHF1 (untagged)-Human PHD finger protein 1 (PHF1), transcript variant 2
  $529.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHF1
Synonyms hPHF1; MTF2L2; PCL1; PHF2; TDRD19C
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_024165.1 GCTGCCGCTGCTTTGGCTGCTGCGTCATACGCCCCAGAGCCGCCGGGACGGAGGGGCTGG
GCCTGGGGACCCCCCGGCCTCCGCCTGCACGCCCCCCCACGCCCGGACGTGCCCTCTCCG
CGCGGGGGACTCGCCTAGGTCTCCTACGTCTGCCCCTGCCCGGCTCCCGGCGGCCCCAGC
TGTCACCGGCCCCCCCAGGATGCAATGGCGCAGCCCCCCCGGCTGAGCCGCTCTGGTGCC
TCCTCACTTTGGGACCCAGCTTCTCCTGCTCCCACCTCTGGCCCCAGGCCTCGGCTTTGG
GAGGGTCAAGATGTGCTGGCCAGATGGACTGATGGGCTGCTATACTTGGGTACCATCAAA
AAGGTGGACAGTGCTAGGGAGGTGTGTCTGGTCCAGTTTGAGGATGATTCGCAGTTTCTG
GTTCTATGGAAAGACATTAGCCCTGCTGCCCTCCCTGGAGAGGAACTCCTCTGTTGTGTC
TGTCGCTCTGAGACTGTGGTCCCTGGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCAT
GCTTATCACCAGGACTGCCATGTTCCCAGGGCTCCAGCCCCTGGAGAGGGAGAGGGCACA
TCCTGGGTATGCCGCCAGTGTGTCTTTGCGATCGCCACCAAGAGGGGAGGTGCCCTGAAG
AAGGGCCCCTATGCCCGGGCCATGCTGGGTATGAAGCTTTCTCTGCCATATGGACTGAAG
GGGCTGGACTGGGATGCTGGACATCTGAGCAACCGACAGCAGAGTTACTGTTACTGTGGT
GGCCCTGGGGAGTGGAACCTGAAAATGCTGCAGTGCCGGAGCTGCCTGCAGTGGTTCCAT
GAGGCCTGCACCCAGTGTCTGAGCAAGCCCCTCCTCTATGGGGACAGGTTCTATGAATTT
GAATGCTGTGTGTGTCGCGGGGGCCCTGAGAAAGTCCGGAGACTACAGCTTCGCTGGGTG
GATGTGGCCCATCTTGTCCTGTATCACCTCAGTGTTTGCTGTAAGAAGAAATACTTTGAT
TTTGATCGTGAGATCCTCCCCTTCACTTCTGAGAATTGGGACAGTTTGCTCCTGGGGGAG
CTTTCAGACACCCCCAAAGGAGAACGTTCTTCCAAGCTCCTCTCTGCTCTTAACAGCCAC
AAGGACCGTTTCATTTCAGGGAGAGAGATTAAGAAGAGGAAATGTTTGTTTGGTCTCCAT
GCTCGGATGCCTCCCCCTGTGGAGCCCCCTACTGGAGATGGAGCACTCACCAGCTTCCCT
TCAGGGCAGGGCCCTGGGGGAGGGGTCTCACGTCCCCTGGGGAAGCGCCGGAGGCCGGAG
CCAGAGCCCCTGAGGAGGAGGCAGAAGGGGAAAGTGGAGGAGCTGGGGCCACCCTCAGCA
GTGCGCAATCAGCCCGAGCCCCAGGAGCAGAGGGAGCGGGCTCATCTGCAGAGGGCACTG
CAGGCCTCAGTGTCTCCACCATCCCCCAGCCCTAACCAGAGTTACCAGGGCAGCAGCGGC
TACAACTTCCGGCCCACAGATGCCCGCTGCCTGCCCAGCAGCCCCATCCGGATGTTTGCT
TCCTTCCACCCTTCTGCCAGCACCGCAGGGACCTCTGGGGACAGTGGACCCCCAGACAGG
TCACCCCTGGAACTTCACATTGGTTTCCCCACAGACATCCCTAAAAGTGCCCCCCACTCG
ATGACTGCCTCATCTTCCTCAGTTTCATCCCCATCCCCAGGTCTTCCTAGACGCTCAGCA
CCCCCTTCTCCCCTGTGCCGTAGTTTGTCTCCTGGGACTGGGGGAGGAGTCCGAGGTGGG
GTTGGTTACCTGTCCCGAGGGGACCCTGTCCGGGTCCTTGCTCGGAGAGTACGGCCTGAT
GGCTCTGTGCAGTACCTGGTTGAGTGGGGAGGAGGGGGCATCTTCTGAACAGCCTGCCTC
TGCCCAGCTCCCCATTCACACACACCGGCACTTTCATACCCTGACCTCTGACCTCACCTA
CAGCTGGGATGTACCTGGAGAGATAGGGGGTAGTTCTCCCTACTGCCCAGGCTGGAATCC
AAGAGTGGGGAGTGGGGAAGAGGCCCTCTTCTCTACCCTCCTTCATGATTCCTGACCCCT
CCCATCCTTCCCATTTCCTTTGATGTTATTTTGTTACAGCTTTTTAAATATTTTTTAAAA
TTATTTAACCCCTGGGGGCAGAGACTGAGGAGGGAGGATGATAAGGGATCCCGGACTCTG
TATGATTGAAATAAAGAGAAATAAACAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_024165
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024165.1, NP_077084.1
RefSeq Size 2260 bp
RefSeq ORF 1704 bp
Locus ID 5252
UniProt ID O43189
Cytogenetics 6p21.32
Domains PHD, TUDOR
Protein Families Druggable Genome, Transcription Factors
Summary This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2009
Transcript Variant: This variant (2), also called variant PHF2, represents the longest transcript and encodes the longer isoform (b).
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Documents
Resources
"NM_024165" in other vectors (7)
SKU Description Size Price
RC202906 PHF1 (Myc-DDK-tagged)-Human PHD finger protein 1 (PHF1), transcript variant 2 10 ug
$528.00
RC202906L1 Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, Myc-DDK-tagged 10 ug
$828.00
RC202906L2 Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, mGFP tagged 10 ug
$828.00
RC202906L3 Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, Myc-DDK-tagged 10 ug
$828.00
RC202906L4 Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, mGFP tagged 10 ug
$828.00
RG202906 PHF1 (tGFP-tagged) - Human PHD finger protein 1 (PHF1), transcript variant 2 10 ug
$728.00
SC112257 PHF1 (untagged)-Human PHD finger protein 1 (PHF1), transcript variant 2 10 ug
$529.00

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