SPIB (NM_003121) Human Untagged Clone

SKU
SC320776
SPIB (untagged)-Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SPIB
Synonyms SPI-B
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_003121.2 CACCATGCTCGCCCTGGAGGCTGCACAGCTCGACGGGCCACACTTCAGCTGTCTGTACCC
AGATGGCGTCTTCTATGACCTGGACAGCTGCAAGCATTCCAGCTACCCTGATTCAGAGGG
GGCTCCTGACTCCCTGTGGGACTGGACTGTGGCCCCACCTGTCCCAGCCACCCCCTATGA
AGCCTTCGACCCGGCAGCAGCCGCTTTTAGCCACCCCCAGGCTGCCCAGCTCTGCTACGA
ACCCCCCACCTACAGCCCTGCAGGGAACCTCGAACTGGCCCCCAGCCTGGAGGCCCCGGG
GCCTGGCCTCCCCGCATACCCCACGGAGAACTTCGCTAGCCAGACCCTGGTTCCCCCGGC
ATATGCCCCGTACCCCAGCCCTGTGCTATCAGAGGAGGAAGACTTACCGTTGGACAGCCC
TGCCCTGGAGGTCTCGGACAGCGAGTCGGATGAGGCCCTCGTGGCTGGCCCCGAGGGGAA
GGGATCCGAGGCAGGGACTCGCAAGAAGCTGCGCCTGTACCAGTTCCTGCTGGGGCTACT
GACGCGCGGGGACATGCGTGAGTGCGTGTGGTGGGTGGAGCCAGGCGCCGGCGTCTTCCA
GTTCTCCTCCAAGCACAAGGAACTCCTGGCGCGCCGCTGGGGCCAGCAGAAGGGGAACCG
CAAGCGCATGACCTACCAGAAGCTGGCGCGCGCCCTCCGAAACTACGCCAAGACCGGCGA
GATCCGCAAGGTCAAGCGCAAGCTCACCTACCAGTTCGACAGCGCGCTGCTGCCTGCAGT
CCGCCGGGCCTGAGCACACCCGAGGCTCCCACCTGCGGAGCCGCTGGGGGACCTCACGTC
CCAGCCAGGATCCCCCTGGAAGAAAAAGGGCGTCCCCACACTCTAGGTGATAGGACTTAC
GCATCCCCACCTTTTGGGGTAAGGGGAGTGCTGCCCTGCCATAATCCCCAAGCCCAGCCC
GGGCCTGTCTGGGATTCCCCACTTGTGCCTGGGGTCCCTCTGGGATTTCTTTGTCATGTA
CAGACTCCCTGGGATCCTCATGTTTTGGGTGACAGGACCTATGGACCACTATACTCGGGG
AGGCAGGGTAGCAGTGCTTCCAGAGTCCCAAGAGCTTCTCTGGGATTTTCTTGTGATATC
TGATTCCCCAGTGAGGCCTGGGACCTTTTTAAGATCGCTGTGTGTCTGTAAACCCTGAAT
CTCATCTGGGGTGGGGGCCCTGCTGGCAACCCTGAGCCCTGTCCAAGGTTCCCTCTTGTC
AGATCTGAGATTTCCTAGTTATGTCTGGGGCCCTCTGGGAGCTGTTATCATCTCAGATCT
CTTCGCCCATCTATGGCTGTGTTGTCACATCTGTCCCCTCATTTTTGAGATCCCCCAATT
CTCTGGAACTATTCTGCTGCCCCTTTTTATGTGTCTGGAGTTCCCCAATCACATCTAGGG
CTCCTCCAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_003121
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003121.2, NP_003112.2
RefSeq Size 1467 bp
RefSeq ORF 789 bp
Locus ID 6689
UniProt ID Q01892
Cytogenetics 19q13.33
Protein Families Transcription Factors
Summary The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2011
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_003121" in other vectors (8)
SKU Description Size Price
RC203170 SPIB (Myc-DDK-tagged)-Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB) 10 ug
$450.00
RC203170L1 Lenti ORF clone of Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), Myc-DDK-tagged 10 ug
$750.00
RC203170L2 Lenti ORF clone of Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), mGFP tagged 10 ug
$750.00
RC203170L3 Lenti ORF clone of Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), Myc-DDK-tagged 10 ug
$750.00
RC203170L4 Lenti ORF clone of Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), mGFP tagged 10 ug
$750.00
RG203170 SPIB (tGFP-tagged) - Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB) 10 ug
$650.00
SC118169 SPIB (untagged)-Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB) 10 ug
$450.00
SC317377 SPIB (untagged)-Human Spi-B transcription factor (Spi-1/PU.1 related) (SPIB) 10 ug
$480.00

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