PTOV1 (NM_017432) Human Untagged Clone

SKU
SC320543
PTOV1 (untagged)-Human prostate tumor overexpressed 1 (PTOV1)
$457.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PTOV1
Synonyms ACID2; PTOV-1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_017432.3 CTCCCCCGAAGCCGCGCGCCCGCGCCCGCGCCCCTCAGTCGGTGGAGCCCGCAGCCCCCC
TTGTGGCCCGCGGCAGCTCCCCGCCCGCTCGGCCCGCGCCCGCCATGGTCCGTCCGCGCC
GTGCCCCGTACCGCTCCGGCGCCGGGGGCCCCCTCGGGGGTCGCGGCCGCCCTCCGCGGC
CCCTCGTGGTGCGCGCCGTCCGCTCGCGCTCCTGGCCTGCCAGCCCCCGAGGCCCGCAGC
CTCCGCGGATCCGGGCCCGCTCGGCCCCTCCCATGGAAGGTGCTCGGGTCTTCGGGGCAC
TGGGTCCCATCGGTCCCTCCTCACCTGGGCTCACCCTCGGGGGTCTGGCCGTGAGCGAGC
ACCGGCTCAGCAACAAGCTGCTGGCTTGGAGCGGCGTCCTCGAGTGGCAGGAGAAGCGCA
GACCCTACTCTGACTCCACTGCAAAGCTGAAGCGGACCCTGCCCTGCCAAGCCTACGTGA
ACCAAGGCGAGAACCTGGAGACCGACCAGTGGCCGCAGAAGCTGATCATGCAGCTGATCC
CTCAGCAGCTGCTGACCACCCTGGGCCCCCTGTTCCGGAACTCCCAGTTGGCACAGTTCC
ACTTCACCAACAGAGACTGCGACTCGCTCAAGGGGCTCTGCCGCATCATGGGCAACGGCT
TCGCGGGCTGCATGCTGTTCCCCCACATCTCCCCCTGTGAGGTGCGCGTGCTCATGCTCC
TGTACTCGTCCAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCG
TCAGTGCCATCCGGCAGGTCATCACCACCCGCAAGCAGGCAGTGGGACCTGGTGGTGTCA
ACTCAGGCCCAGTCCAGATCGTCAACAACAAGTTTCTGGCATGGAGTGGTGTCATGGAGT
GGCAGGAGCCCAGGCCTGAGCCCAACAGTCGGTCCAAGAGGTGGCTGCCATCCCACGTCT
ACGTGAACCAGGGGGAGATCCTGAGGACCGAGCAGTGGCCAAGGAAGCTGTACATGCAGC
TCATCCCGCAGCAGCTGCTGACCACCCTAGTGCCGCTGTTCCGGAACTCGCGCCTGGTCC
AGTTCCACTTCACCAAGGACCTGGAGACACTGAAGAGCCTGTGCCGGATCATGGACAATG
GCTTCGCCGGCTGCGTGCACTTTTCCTACAAAGCATCGTGTGAGATCCGCGTGCTTATGC
TCCTGTACTCTTCAGAGAAGAAAATCTTCATTGGCCTCATCCCCCATGACCAGGGCAACT
TTGTCAACGGCATCCGGCGTGTCATTGCCAACCAGCAGCAGGTCCTGCAGCGGAACCTGG
AGCAGGAGCAACAGCAACGAGGGATGGGGGGGTAGTGGTTACCCCGGGCTGGGCCCCTCC
AGGAGTCACAGATGAGGCCCCCGCAGAGACTGTGCTTGGGAGCCTGTGGCCAGCAGCCCC
CACTGTACCCCTGCCCCCAGGTGACACGCTTCTGAGCAGGGGCCCCTGGGGACTTCAACT
GCCCAGCAACATGGAGGATGGTGTCCTGAGGCCTCCAAGGACGGTCCCCACCCCTCTACG
TTTCCCCAATAAAGCCTTTTAAAAACCAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire
ACCN NM_017432
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017432.3, NP_059128.2
RefSeq Size 1884 bp
RefSeq ORF 1251 bp
Locus ID 53635
UniProt ID Q86YD1
Cytogenetics 19q13.33
Protein Families Druggable Genome
Summary This gene encodes a protein that was found to be overexpressed in prostate adenocarcinomas. The encoded protein was found to interact with the lipid raft protein flotillin-1 and shuttle it from the cytoplasm to the nucleus in a cell cycle dependent manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This transcript contains splicing in the 3' UTR that makes it a candidate for nonsense-mediated mRNA decay (NMD). However, this protein is thought to be valid.
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Citations

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