NARS2 (NM_024678) Human Untagged Clone

SKU SC319213

NARS2 (untagged)-Human asparaginyl-tRNA synthetase 2, mitochondrial (putative) (NARS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NARS2
• Synonyms: asnRS; DFNB94; SLM5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024678.3 GAGAGGTTCTGTGGGGACAGAGCTGGGAGTCCTGAAGGAGAAAGCGCGCAGCCGAGGGGC; TCTGGAGTGCCTTAGAGCAGAGCTGCGGCCGCGGAAGGGGAGCAGCTGAGAAAGGAGGGC; CGCTGCAGGCGGGGTTCGAACCGTGGGGTCTGGGCTGCTCCCGCGGAGGGCCTGGGCGGA; CGCGGGATGCTGGGGGTCCGCTGCCTGCTGCGGTCCGTGCGCTTCTGTTCCTCCGCCCCC; TTCCCCAAGCACAAACCTTCAGCCAAACTGAGCGTGCGGGACGCTCTCGGGGCTCAGAAC; GCGAGTGGGGAGCGCATTAAGATCCAGGGATGGATTCGTTCTGTCCGATCCCAGAAGGAA; GTCTTGTTCCTGCATGTAAATGATGGGTCATCTTTGGAAAGCCTTCAGGTTGTTGCAGAT; TCAGGCCTTGACAGTAGAGAATTAACTTTTGGGAGTTCTGTGGAAGTACAAGGGCAGCTG; ATAAAAAGTCCATCCAAAAGGCAAAATGTGGAACTGAAGGCAGAAAAAATTAAAGTTATT; GGAAATTGTGATGCCAAGGATTTCCCCATCAAATATAAAGAGAGGCATCCTCTGGAGTAC; CTGCGACAATATCCTCACTTTAGGTGTAGGACTAACGTTCTGGGTTCTATATTGAGGATT; CGCAGTGAAGCGACAGCTGCTATTCATTCTTTCTTTAAGGACAGTGGCTTTGTACATATT; CATACTCCAATAATCACATCCAATGACTCTGAGGGAGCTGGAGAACTTTTTCAACTTGAA; CCTTCAGGCAAACTTAAGGTACCTGAGGAGAATTTCTTCAATGTTCCTGCTTTCTTAACT; GTCTCAGGACAACTTCATCTAGAAGTGATGTCAGGAGCTTTTACTCAAGTGTTTACCTTT; GGTCCGACCTTCCGAGCTGAAAATTCTCAGAGCCGGAGGCACCTGGCAGAGTTTTATATG; ATAGAAGCAGAGATTTCTTTTGTTGACAGCCTTCAAGATCTTATGCAGGTTATAGAGGAA; CTGTTCAAGGCTACAACAATGATGGTTCTCTCAAAATGTCCTGAAGATGTTGAACTCTGT; CACAAATTCATAGCACCTGGCCAAAAGGACAGATTAGAACATATGCTAAAAAACAACTTT; TTAATCATTTCTTATACTGAAGCAGTGGAGATCTTAAAGCAAGCATCCCAGAACTTCACC; TTTACCCCAGAGTGGGGTGCTGACCTACGGACTGAACATGAAAAGTACCTGGTGAAGCAC; TGTGGCAACATACCTGTCTTCGTTATTAATTATCCATTAACACTCAAGCCTTTCTACATG; AGGGATAATGAAGATGGCCCTCAGCACACGGTTGCTGCTGTTGATCTTCTGGTTCCTGGA; GTTGGGGAACTCTTTGGAGGAGGCCTCAGAGAAGAACGATACCATTTCTTAGAGGAGCGC; TTAGCCAGATCGGGACTTACAGAAGTCTACCAATGGTATCTGGACCTTCGTCGATTTGGA; TCTGTGCCACATGGAGGTTTTGGGATGGGATTTGAACGCTACCTGCAGTGCATCTTGGGT; GTTGACAATATCAAAGATGTTATCCCTTTCCCAAGGTTTCCTCATTCATGCCTTTTATAG; CTGGAAGATTGGTTAAGGAAAAGCACCCCCCATGGCAGAGACACTGCACATGATTGTGCA; TACAGCAGAATGCATGTTTGGATTTTAGAAATGCAGATTTCAATATGTAATTGTTGTGCC; ATAAGATATCATAGAAAAAATATAAGTGGTTGTGATTTTCTTAGAAAGTTGAGGGTATTT; CACGTAAGGATGAGCTCCCGCAAGAAGAGGTACTTATAGCAAGGGGACTCTCAAATCCAT; TACCTCAATTAAGAAATGAAGAAATTGAATTAGTCTCAAAGTTTCTTTTAAACTCTAAAA; CAGAATGAGATAATGTATTTTACGTTGTCTATAATCATTAAATCACTCCCTGTGTAATTT; GTGAGAACCATCTAGTAGCTCGAAATAAAATAATGTTGCATCTTTTCTCCCTTGCCATAT; ACTTTGTGATAAATCCTTATCTCATTTTCAGTACTTCATTAAACATTGCAGAAAAAAAAA; AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_024678
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024678.3, NP_078954.3
• RefSeq Size: 2110 bp
• RefSeq ORF: 1434 bp
• Locus ID: 79731
• UniProt ID: Q96I59
• Cytogenetics: 11q14.1
• Domains: tRNA-synt_2, tRNA_anti
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Summary: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24). [provided by RefSeq, Mar 2015]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).