MMS19 (NM_022362) Human Untagged Clone

SKU SC318470

MMS19 (untagged)-Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MMS19
• Synonyms: CIAO4; hMMS19; MET18; MMS19L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318470 representing NM_022362.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCCGCTGCCGCGGCTGTGGAGGCGGCGGCGCCTATGGGTGCCCTATGGGGCCTCGTGCACGACTTC; GTCGTGGGTCAGCAAGAGGGCCCCGCTGACCAGGTGGCTGCAGATGTGAAATCTGGCAACTATACAGTG; TTACAAGTTGTGGAAGCCCTTGGGTCCTCTCTAGAGAATCCAGAACCCCGAACTCGGGCACGAGCAATC; CAGCTTTTGTCACAGGTGCTACTCCACTGTCACACCTTGCTCCTGGAGAAGGAAGTGGTACACCTGATA; CTGTTCTATGAGAACCGGCTGAAGGACCATCATCTTGTGATCCCATCTGTCCTGCAGGGTTTGAAGGCA; CTTAGCCTGTGTGTGGCCCTGCCCCCAGGGCTGGCTGTTTCTGTGCTTAAAGCCATCTTCCAGGAAGTG; CATGTACAGTCCCTGCCACAGGTGGACCGACACACAGTCTACAATATCATCACCAATTTTATGCGAACC; CGGGAAGAAGAGCTAAAGAGCCTAGGAGCTGACTTCACCTTTGGCTTCATCCAGGTGATGGATGGGGAA; AAGGATCCCCGTAATCTTCTGGTGGCCTTCCGCATCGTCCATGACCTCATCTCCAGGGACTATAGCCTG; GGACCCTTTGTGGAGGAGTTGTTTGAAGTGACATCCTGTTATTTCCCTATCGATTTTACCCCTCCACCT; AATGATCCCCATGGTATCCAGAGAGAAGACCTCATCCTGAGTCTTCGCGCTGTGCTGGCTTCTACACCA; CGATTTGCTGAGTTTCTGCTGCCCCTGTTGATTGAGAAAGTGGATTCTGAGGTTCTGAGTGCCAAGTTG; GATTCTCTACAGACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAACTGAAGGACTTCCTCCCC; AGCCTTTGGGCTTCTATCCGCAGAGAGGTGTTCCAGACGGCAAGTGAGCGGGTGGAGGCAGAGGGCCTG; GCGGCCCTCCACTCCCTGACTGCGTGTTTGTCTCGCTCTGTGCTGAGGGCTGATGCTGAGGACCTCCTT; GACTCCTTCCTTAGCAACATTCTACAGGACTGCAGGCACCACCTGTGTGAACCGGACATGAAACTGGTG; TGGCCTAGTGCCAAGCTGTTGCAGGCAGCTGCAGGTGCATCTGCCCGGGCCTGTGACTCTGTCACCAGC; AATGTACTGCCTTTACTGCTGGAACAGTTCCACAAGCACAGTCAGAGCAGCCAGCGGCGGACAATCCTT; GAAATGCTCCTGGGTTTCTTGAAGCTGCAGCAGAAATGGAGCTATGAAGACAAAGATCAAAGGCCTCTG; AATGGCTTCAAGGACCAGCTGTGCTCACTGGTATTCATGGCTCTAACAGACCCCAGCACCCAGCTTCAG; CTTGTTGGCATCCGTACACTGACAGTCTTGGGTGCCCAGCCAGATCTCCTATCTTATGAGGACTTGGAG; CTGGCAGTGGGTCACCTGTACAGACTGAGCTTCCTGAAGGAGGATTCCCAGAGTTGCAGGGTGGCAGCA; CTGGAAGCATCAGGAACCCTGGCTGCTCTCTACCCTGTGGCCTTCAGCAGCCACCTCGTACCCAAGCTC; GCTGAGGAGCTGCGTGTAGGGGAGTCAAATTTGACTAACGGAGATGAGCCCACCCAATGCTCCCGGCAT; CTGTGCTGTCTGCAAGCCTTGTCAGCTGTATCAACACATCCCAGCATCGTCAAGGAGACACTGCCTCTG; CTGCTGCAGCATCTCTGGCAAGTGAACAGAGGGAATATGGTTGCACAATCCAGTGACGTTATTGCTGTC; TGTCAGAGCCTCAGACAGATGGCAGAAAAATGTCAGCAGGACCCTGAGAGTTGCTGGTATTTCCACCAG; ACAGCTATACCTTGCCTGCTTGCCTTGGCTGTGCAGGCCTCTATGCCAGAGAAGGAGCCCTCAGTTCTG; AGAAAAGTACTATTGGAGGATGAGGTGTTGGCTGCCATGGTGTCTGTCATTGGCACTGCTACAACCCAC; CTGAGCCCTGAGTTAGCTGCCCAGAGTGTGACACACATTGTGCCCCTCTTCTTGGATGGCAACGTGTCC; TTTCTGCCTGAAAACAGCTTCCCGAGCAGATTCCAGCCATTCCAGGATGGCTCCTCAGGGCAGAGGCGG; CTGATTGCACTGCTTATGGCCTTTGTCTGCTCCCTGCCTCGAAATGTGGAAATCCCTCAGCTGAACCAA; CTCATGCGGGAGCTTTTGGAACTGAGCTGCTGCCACAGCTGCCCCTTTTCTTCCACCGCTGCTGCCAAG; TGCTTTGCAGGACTCCTCAACAAGCACCCTGCAGGGCAGCAGCTGGATGAATTCCTACAGCTAGCTGTG; GACAAAGTGGAGGCTGGCCTGGGCTCTGGGCCCTGTCGTAGTCAGGCCTTCACTCTTCTTCTCTGGGTA; ACAAAGGCCCTAGTGCTCAGATACCATCCTCTCAGCTCCTGCCTTACAGCCCGGCTCATGGGCCTCCTG; AGTGACCCAGAATTAGGTCCAGCAGCAGCTGATGGCTTCTCTCTGCTCATGTCTGACTGCACTGATGTG; CTGACTCGTGCTGGCCATGCCGAAGTGCGGATCATGTTCCGCCAGCGGTTCTTCACAGATAATGTGCCT; GCTTTGGTCCAGGGCTTCCATGCTGCTCCCCAAGATGTGAAGCCAAACTACTTGAAGGGTCTTTCTCAT; GTACTTAACAGGCTGCCCAAGCCTGTACTCTTGCCAGAGCTGCCCACGCTTCTTTCCTTGCTGCTGGAG; GCCCTGTCCTGCCCTGACTGTGTGGTGCAGCTCTCCACCCTCAGCTGCCTTCAGCCTCTTCTACTGGAA; GCACCCCAAGTCATGAGTCTTCACGTGGACACCCTCGTCACCAAGTTTCTGAACCTCAGCTCTAGCCCT; TCCATGGCTGTCCGGATCGCCGCACTGCAGTGCATGCATGCTCTCACTCGCCTGCCCACCCCTGTGCTG; CTGCCGTACAAACCACAGGTGATTCGGGCCTTAGCCAAACCCCTGGATGACAAGAAGAGACTGGTGCGC; AAGGAAGCAGTGTCAGCCAGAGGGGAGTGGTTTCTGTTGGGGAGCCCTGGCAGCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_022362
• Insert Size: 3093 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022362.4
• RefSeq Size: 3703 bp
• RefSeq ORF: 3093 bp
• Locus ID: 64210
• UniProt ID: Q96T76
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 113.3 kDa
• Summary: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034, PubMed:23585563). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 4 encode the same protein. Sequence Note: A downstream start codon is selected for this RefSeq based on better conservation with homologous proteins. The use of an alternative upstream start codon, which is present in some species, including human, chimp and macaque (with a subsequent CDS frameshift in the latter species), would increase the protein length from 1030 aa to 1051 aa. The shorter protein is referred to in the literature, including PMIDs 11071939, 11279242 and 11328871.