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MRTFB (NM_014048) Human Untagged Clone

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SKU
SC318468
MKL2 (untagged)-Human MKL/myocardin-like 2 (MKL2)
  $914.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRTFB
Synonyms MKL2; MRTF-B; NPD001
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318468 representing NM_014048.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGATCACACAGGGGCGATAGACACCGAGGATGAAGTGGGACCTTTAGCCCATCTTGCTCCAAGTCCT
CAGAGTGAAGCTGTGGCTCATGAATTCCAGGAACTCTCCTTGCAGTCCAGTCAAAACTTACCCCCTCTG
AACGAAAGGAAAAATGTGCTCCAGCTGAGGCTGCAACAAAGGAGGACGAGAGAACAACTAGTGGACCAG
GGCATCATGCCACCTTTGAAGAGCCCAGCGGCATTCCATGAACAGATAAAAAGCTTGGAACGAGCCAGA
ACTGAAAACTTTTTGAAACACAAGATTCGGAGTCGACCAGATCGTTCTGAACTTGTCAGGATGCACATT
TTAGAAGAAACATTTGCAGAGCCATCCCTGCAGGCTACTCAGATGAAGTTGAAAAGAGCTCGACTAGCA
GATGATCTGAATGAAAAGATTGCTCAAAGACCTGGTCCTATGGAGCTGGTAGAGAAAAACATCCTTCCT
GTGGACTCCAGTGTTAAAGAAGCAATTATAGGCGTTGGGAAGGAGGACTATCCCCACACTCAGGGCGAT
TTCTCATTTGATGAAGACAGCAGTGACGCTTTGTCTCCGGACCAGCCTGCGAGTCAGGAGTCACAGGGG
TCAGCCGCGTCCCCAAGTGAGCCAAAAGTTAGTGAATCGCCATCTCCTGTGACTACAAACACTCCAGCG
CAGTTTGCTTCAGTGTCCCCAACAGTTCCTGAATTCTTGAAAACTCCTCCAACTGCAGATCAGCCTCCC
CCACGGCCTGCAGCTCCTGTCCTCCCCACAAACACTGTGTCCTCAGCAAAGCCTGGCCCAGCACTGGTG
AAGCAAAGCCATCCCAAGAATCCAAATGACAAACACCGTAGCAAAAAGTGCAAAGATCCCAAACCACGG
GTAAAGAAGTTAAAGTACCACCAATACATTCCACCAGATCAGAAGGGTGAGAAGAATGAGCCGCAGATG
GACTCTAACTACGCCCGCCTGCTCCAGCAGCAGCAGCTGTTCCTGCAACTGCAGATCCTGAGTCAGCAG
AAGCAGCACTACAACTACCAGACCATCCTGCCTGCACCATTCAAGCCACTCAATGACAAAAATAGTAAC
AGTGGGAATTCAGCTTTGAACAATGCCACACCTAACACACCAAGACAGAATACATCTACTCCTGTGAGA
AAGCCAGGACCTCTGCCTTCTAGCCTGGATGACTTAAAGGTATCAGAACTGAAGACAGAACTGAAGTTA
AGGGGTCTGCCAGTGTCAGGCACCAAACCGGACCTCATTGAGCGCCTAAAACCCTACCAGGAAGTGAAC
AGCAGCGGCCTTGCTGCTGGGGGCATCGTGGCAGTGTCATCATCAGCCATTGTCACCAGTAACCCAGAA
GTCACTGTGGCCTTGCCGGTTACAACACTACACAACACTGTGACTAGCTCAGTCTCTACTCTCAAGGCA
GAATTGCCACCTACAGGAACCAGCAACGCAACCCGTGTGGAAAATGTTCATTCCCCTCTGCCCATTTCA
CCATCTCCCTCCGAACAGTCCAGTCTCAGTACTGATGACACAAACATGGCAGACACTTTCACCGAGATT
ATGACCATGATGTCGCCTTCACAGTTCTTGAGTTCATCTCCTTTGAGAATGACAAATAATGAAGACAGT
CTGAGTCCCACCAGCAGCACTCTGTCAAACCTGGAACTGGATGCAGCCGAAAAGGATCGCAAGCTTCAG
GAGAAAGAGAAGCAAATCGAAGAGCTGAAGAGGAAACTGGAACAAGAGCAGAAGCTCGTGGAAGTGCTG
AAAATGCAACTTGAGGTTGAAAAACGAGGGCAGCAGCAGCGGCCCCTGGAAGCCCAGCCCAGTGCCCCA
GGTCATTCTGTCAAGTCAGATCAGAAGCACGGCAGCCTTGGCTCCTCCATCAAAGATGAGGCCTCACTC
CCTGACTGCTCCAGCTCCAGGCAGCCCATCCCAGTAGCCAGCCACGCTGTAGGCCAGCCCGTCTCTACA
GGTGGCCAGACCCTTGTTGCCAAAAAGGCTGTAGTTATCAAGCAAGAGGTCCCTGTGGGCCAGGCAGAG
CAGCAGAGTGTCGTCTCGCAGTTTTATACTTCACCACAAGCAGGAATGCAGACTCAGCCTCAGATAGCA
ACTGCTGCACAAATACCAACTGCTGCCTTGGCCTCAGGCTTGGCCCCAACTGTACCTCAGACACAAGAC
ACGTTCCCGCAGCATGTGCTCAGTCAGCCTCAACAAGTCAGAAAGGTTTTCACAAACTCAGCATCATCA
AATACAGTTCTTCCATATCAGAGACATCCTGCCCCAGCTGTCCAGCAGCCCTTTATCAATAAGGCCTCC
AACAGTGTTCTTCAATCCAGAAATGCTCCGCTTCCATCCCTGCAAAATGGACCTAACACACCCAACAAG
CCTAGTTCACCCCCGCCACCCCAGCAATTTGTCGTCCAGCACTCTCTATTTGGGAGTCCAGTCGCCAAG
ACAAAAGATCCCCCCCGCTATGAGGAGGCCATCAAGCAGACACGCAGCACACAGGCCCCTCTGCCAGAG
ATTTCCAACGCTCACAGTCAGCAGATGGATGACCTCTTTGATATCCTCATTAAGAGTGGAGAGATCTCC
CTCCCCATAAAAGAAGAACCTTCTCCTATTTCCAAAATGAGACCAGTGACAGCCAGCATCACCACAATG
CCAGTGAATACAGTGGTGTCCCGGCCACCACCCCAAGTCCAAATGGCACCACCTGTATCTTTAGAACCT
ATGGGCAGTTTATCTGCCAGCTTAGAGAACCAACTAGAAGCTTTCTTGGATGGAACTTTACCCTCAGCC
AATGAAATTCCTCCACTACAAAGCAGCAGTGAAGACAGAGAGCCCTTCTCTCTGATCGAGGACCTCCAG
AATGATCTGCTGAGTCACTCAGGTATGCTGGACCATTCACACTCACCCATGGAGACTTCCGAGACCCAG
TTTGCTGCAGGTACTCCCTGTCTGTCTCTCGACCTGTCAGACTCAAACTTGGACAACATGGAGTGGTTG
GACATTACCATGCCCAACTCCTCTTCAGGACTCACTCCTCTCAGCACCACCGCGCCGAGCATGTTCTCT
GCTGACTTTCTAGACCCACAGGACCTACCGCTGCCATGGGACTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_014048
Insert Size 3150 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014048.3
RefSeq Size 8680 bp
RefSeq ORF 3150 bp
Locus ID 57496
UniProt ID Q9ULH7
Cytogenetics 16p13.12
Protein Families Transcription Factors
MW 114.1 kDa
Summary Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_014048" in other vectors (5)
SKU Description Size Price
RC215687 MKL2 (Myc-DDK-tagged)-Human MKL/myocardin-like 2 (MKL2) 10 ug
$913.00
RC215687L3 Lenti ORF clone of Human MKL/myocardin-like 2 (MKL2), Myc-DDK-tagged 10 ug
$1,213.00
RC215687L4 Lenti ORF clone of Human MKL/myocardin-like 2 (MKL2), mGFP tagged 10 ug
$1,213.00
RG215687 MKL2 (tGFP-tagged) - Human MKL/myocardin-like 2 (MKL2) 10 ug
$789.00 $1,113.00
SC115193 MKL2 (untagged)-Human MKL/myocardin-like 2 (MKL2) 10 ug
$150.00

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