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TLE4 (NM_007005) Human Untagged Clone

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SKU
SC318250
TLE4 (untagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4)
  $709.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TLE4
Synonyms BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
Vector PCMV6-Neo
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_007005 edited
ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCT
GCTCAACCCTTTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAG
TTTTTACAGGCTCAATACCACAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAG
ACAGAGATGCAGCGGCATTATGTCATGTATTATGAAATGTCCTATGGGTTGAATATAGAA
ATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTATCTGTGCACAAGTCATTCCT
TTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGCCAAGCAGGTG
ACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACAT
GGACATGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATT
CCACCCATCGGTAGCAGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCC
CATCTTCCAATTAAAGATGAGAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGAC
TCCATCAAGAGCTCTTCAGTATCCCCATCAGCCAGTTTCCGAGGTGCTGAGAAGCACAGA
AACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAACTGAAGAAAAGGAAATTGCA
GCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGACGTTTCCAAT
GAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGAC
AAGACACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGC
AGTACTCCCTCCTCCAAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTC
TCAAAGTCCAATACCCCTACTCCACGAACTGATGCGCCCACCCCAGGCAGTAACTCTACT
CCCGGATTGAGGCCTGTACCTGGAAAACCACCAGGAGTTGACCCTTTGGCCTCAAGCCTA
AGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCATTTGGGATTGTGCCCCATGCT
GGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCACAACATCTCC
CCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCA
GTGGTGGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACA
GGCATTCCAGGAGGAAAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAG
CCTGTCCCTTTTCCACCCGACGCCCTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAG
ATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCGGTGACCATCAGCAACCCCACGAGA
CACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCAGGCAAT
AAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTACATCCGTTCCTGCAGA
TTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGG
GACCTGGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGC
TATGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAAC
ATCGCTGTGTGGGATCTGCACAACCAGACCTTGGTGAGGCAATTCCAGGGCCACACAGAT
GGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACAGGTGGTTTGGAC
AACACGGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACC
TCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAGTGGCTTGCAGTGGGGATGGAG
AACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAACTACATCTTCAT
GAGAGCTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGA
AAGGACAACCTTCTGAATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAA
GAATCCTCATCGGTGCTTAGCTGTGACATCTCCGTGGACGACAAATACATTGTCACTGGC
TCTGGGGATAAGAAGGCCACAGTTTATGAAGTTATTTATTAA
Restriction Sites Please inquire |
ACCN NM_007005
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007005.3, NP_008936.2
RefSeq Size 4749 bp
RefSeq ORF 2322 bp
Locus ID 7091
UniProt ID Q04727
Cytogenetics 9q21.31
Protein Families Transcription Factors
Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_007005" in other vectors (7)
SKU Description Size Price
RC220536 TLE4 (Myc-DDK-tagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4) 10 ug
$708.00
RC220536L1 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), Myc-DDK-tagged 10 ug
$1,008.00
RC220536L2 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), mGFP tagged 10 ug
$1,008.00
RC220536L3 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), Myc-DDK-tagged 10 ug
$1,008.00
RC220536L4 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), mGFP tagged 10 ug
$1,008.00
RG220536 TLE4 (tGFP-tagged) - Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4) 10 ug
$908.00
SC115738 TLE4 (untagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4) 10 ug
$150.00

file-alt Citations

Dysfusion transcriptional control of Drosophila tracheal migration, adhesion, and fusion
Jiang, Crews
Mol Cell Biol (2006) 26 (17), 6547-56 DOI: 10.1128/MCB.00284-06
AI Summary: OriGene products, specifically the CG13196 cDNA clone, were used in this study to misexpress the CG13196 gene in Drosophila, helping to demonstrate its role in tracheal cell adhesion and ectopic fusion events.
Showing 1-1 of 1 papers.
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