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TLE4 (NM_007005) Human Untagged Clone

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SKU
SC115738
TLE4 (untagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4)
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TLE4
Synonyms BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_007005, the custom clone sequence may differ by one or more nucleotides


ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCTGCTCAACCCT
TTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA
CAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT
TATGAAATGTCCTATGGGTTGAATATAGAAATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTA
TCTGTGCACAAGTCATTCCTTTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGC
CAAGCAGGTGACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACAT
GGACATGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATTCCACCCATCG
GTAGCAGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCCCATCTTCCAATTAAAGATGA
GAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCAGTATCCCCATCA
GCCAGTTTCCGAGGTGCTGAGAAGCACAGAAACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAA
CTGAAGAAAAGGAAATTGCAGCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGA
CGTTTCCAATGAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGAC
AAGACACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGCAGTACTCCCT
CCTCCAAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTCTCAAAGTCCAATACCCCTAC
TCCACGAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCGGATTGAGGCCTGTACCTGGAAAACCA
CCAGGAGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCAT
TTGGGATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCA
CAACATCTCCCCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCA
GTGGTGGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACAGGCATTCCAG
GAGGAAAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAGCCTGTCCCTTTTCCACCCGA
CGCCCTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTG
TGCGCGGTGACCATCAGCAACCCCACGAGACACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTCTGGG
ACATCAGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTACATCCG
TTCCTGCAGATTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGG
GACCTGGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGCTATGCCCTGG
CCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCA
CAACCAGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAGCCAGCTGTATTGACATTTCTAATGAT
GGCACCAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGC
TGCAGCAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAGTGGCTTGCAGT
GGGGATGGAGAACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAACTACATCTTCAT
GAGAGCTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACC
TTCTGAATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAAGAATCCTCATCGGTGCTTAG
CTGTGACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTGGGGATAAGAAGGCCACAGTTTATGAA
GTTATTTATTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_007005 unedited
NGGTGTTCAAATTTTCGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGCCGC
ATCTAAAACCAAGGATTTCAGATACTCATTGCAGTTGTGGAGTTTAATCCCCTTTCTTAA
CCTCACTTCCCACTTGCTATTGAATTGTGAATAGTCATTAAAAACCTGTGATACCAAATC
TTCAGCTGTCTACTTGGAAGAACATGGAATAAGCATACTTAACAGTGAAAAGAATCTTTA
ATTATGTATTATATCTGTAATATATTTATTTTGTTTAAAGAAGGCTTTCTAACAATGACT
GACTAAATAAAGCTGTCTGCTCCTGCATTGATAATGAAGGTGCGTTGTATTTGATACCCC
TCCCCCCCTTTTTTTGGCAAAGGAGGGGAAAGGAAGGTTTAAAATAATTGATTTAAAATG
TCACTAAGTGTAGACTGATGACTGTATAGAGATGTGAAATGTATAATTACACATGGAAGC
AATATGTTGCTGTGTTGTTATTAGGTTTTTTTTGTTTTTGTTTTCTACATCTTTTAAAGA
CTTTTGGAAATTTGGCTGAACAATTAGAACACAACAGGCCAACTCATACTCATTTGGATC
TATTTAGACAACGTTAACCAATATATCTATAGCTTTAGATTATATTCGATAAAAGTAATT
GGACTTTTTTTCTTTTTTTGACTCATTGACAAGTGTCTTTGTAATATGTTTTTAGTTCCC
TTTTTTTGTTGTATTATAGGCAGATGAACAAATTAAATTTGGCCTCAAAGAGAGAACTTA
CTCCCTTCTGNATATTTTTGCCACATTTCTTTGCAAAAGGAGAATATATATCTTTAGTCA
GTTNTGNTGNTATGAGAAACTATGGGTTATTTTGTGGCATGCTCTTTTGGAGCTGCACAG
T
3' Read Nucleotide Sequence
>OriGene 3' read for NM_007005 unedited
GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGAAGTTATTTATGT
TTAATGCTTAAAAGTCTGAATTCACAAACAATCTACCATTATAGAAGTACTGGTGGTCAA
TACAATGCATTAGAACTATGTACAACGCACAGTTTAGTATCAAAATCTTTCTACACTGTA
GAGTTTTACGAAACTGTTAATGACATCAAACACTAAGCACTTAAGACACCATTTTTTTTT
TTTCTGCTACCACATTAGGAACGTCAATGGACAGTCCATTTCAACTTGCAGCATCCATCC
ATTTCTAGTATGAAATTAAGTAATTTTCTACTTATACAATAAAGTATATCTACACGGTTC
TTTTGATTTTGATCCATAGCAGCAAAGGCACTGTACATCAGCAGATCCACAGACTTAAAA
GATTTTTAAAGCATTCAAACAAAAAATAATAAAAAAAGAAGTCACATATTATAGTTTAAC
AGCAACAACAACAACATAAAAGATAACACAATGTTTCTGGCACAATGGCACAGCCTGTGT
CCATTTCAGGGACATCCAACTAAGACATGGAAAGAACCGGAGGTAAAGAAAGCAAGTCCT
CATCCCGGAGAGAGTCCATTCTTTGTGTTTTTCAGAGGGAGGGCTCTGCAACCCTCCTCT
GCTGTCCTTGTTTGTAAAAGACTTACTTTGACAGAGTGGGGATTCCTCCCATAACTGGGC
AGCTCCCAAAAACATGCCCAAAAATACCCCTTATTTTCTCATACAACAAAACTGCTTAAG
AATTATTTTTTTTCCTTTTGCAAAAAAAGTTGGCAAAATTCCCAGAGGGGATTAATTTCT
CTTTTTGAGGGCAAAATTAATTTGTTACACTGCCTTTTATAACACCAAAAAGGGGACTTA
AAACTATTTCCAAGGCCCTTGTCAATGGATCAAAAGAAAAAAAGTC
Restriction Sites NotI-NotI |
ACCN NM_007005
Insert Size 1600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007005.1, NP_008936.1
RefSeq Size 5078 bp
RefSeq ORF 255 bp
Locus ID 7091
UniProt ID Q04727
Cytogenetics 9q21.31
Protein Families Transcription Factors
Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_007005" in other vectors (7)
SKU Description Size Price
RC220536 TLE4 (Myc-DDK-tagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4) 10 ug
$708.00
RC220536L1 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), Myc-DDK-tagged 10 ug
$1,008.00
RC220536L2 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), mGFP tagged 10 ug
$1,008.00
RC220536L3 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), Myc-DDK-tagged 10 ug
$1,008.00
RC220536L4 Lenti ORF clone of Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4), mGFP tagged 10 ug
$1,008.00
RG220536 TLE4 (tGFP-tagged) - Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4) 10 ug
$908.00
SC318250 TLE4 (untagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4) 10 ug
$709.00

file-alt Citations

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