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BBS10 (NM_024685) Human Untagged Clone

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SKU
SC318204
BBS10 (untagged)-Human Bardet-Biedl syndrome 10 (BBS10)
  $729.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BBS10
Synonyms C12orf58
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318204 representing NM_024685.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTTAAGTTCTATGGCCGCTGCAGGGTCTGTGAAGGCGGCGTTGCAGGTGGCCGAGGTGCTGGAAGCC
ATCGTGAGCTGCTGCGTGGGGCCCGAGGGACGGCAAGTTTTGTGTACGAAGCCCACTGGCGAGGTGCTT
CTCAGCCGGAATGGAGGCCGCCTCCTGGAGGCGCTACACTTAGAGCATCCCATAGCCAGGATGATAGTG
GACTGTGTTTCCAGTCATCTCAAAAAAACAGGAGATGGTGCAAAAACATTTATTATCTTTCTTTGCCAT
TTGCTTAGAGGACTTCATGCAATCACAGACAGAGAAAAGGATCCTTTGATGTGTGAAAACATTCAAACC
CATGGAAGGCATTGGAAAAATTGTTCTCGGTGGAAATTTATTTCCCAGGCTCTCCTAACGTTTCAGACA
CAAATATTAGACGGTATTATGGACCAGTACCTAAGTAGACACTTTTTGTCTATCTTTTCGTCTGCTAAA
GAGAGAACATTGTGTAGGAGCTCTTTAGAGTTGCTCTTAGAAGCATACTTTTGTGGAAGAGTGGGAAGA
AATAATCATAAATTTATTTCACAGTTGATGTGTGACTACTTTTTCAAGTGTATGACTTGTAAAAGTGGG
ATTGGTGTATTTGAGTTAGTGGATGACCATTTTGTAGAGTTGAATGTTGGTGTCACTGGCCTTCCTGTT
TCAGATTCCAGGATCATAGCTGGTCTTGTGCTTCAGAAAGATTTTTCTGTGTACCGCCCAGCAGATGGT
GACATGCGAATGGTGATAGTAACAGAAACCATTCAGCCTCTTTTTTCCACTTCTGGATCAGAGTTTATT
CTAAATTCAGAAGCACAGTTTCAGACATCTCAATTTTGGATTATGGAAAAGACAAAAGCAATAATGAAA
CATCTACATAGTCAGAATGTAAAATTGCTCATATCTAGTGTGAAACAACCAGATTTAGTTAGTTATTAT
GCAGGGGTGAATGGCATATCAGTGGTTGAGTGTTTATCATCAGAAGAAGTTTCTCTTATCCGGAGGATC
ATTGGTCTTTCTCCATTTGTACCACCACAGGCCTTTTCGCAGTGTGAAATACCTAACACTGCTTTGGTG
AAATTTTGTAAACCTCTTATCCTTAGATCCAAAAGATATGTTCATCTAGGCTTGATAAGCACATGTGCA
TTTATACCACACTCTATAGTTCTTTGTGGACCAGTGCATGGTCTCATTGAACAACATGAGGATGCTTTA
CATGGAGCACTTAAAATGCTTCGGCAATTATTTAAAGACCTTGATCTAAATTACATGACACAAACCAAT
GACCAAAATGGCACTTCAAGTCTTTTTATTTATAAGAACAGTGGAGAAAGTTATCAAGCACCAGATCCT
GGTAATGGCTCAATACAAAGGCCTTATCAGGACACAGTTGCAGAGAACAAAGATGCATTGGAAAAAACT
CAAACATATTTAAAAGTACATTCTAATTTGGTAATTCCAGATGTAGAATTAGAAACATATATTCCGTAT
TCAACCCCCACACTGACACCAACAGATACATTCCAAACAGTTGAAACGCTGACATGTTTGTCTTTGGAA
AGAAACAGGCTAACTGATTATTATGAACCATTACTCAAGAACAATTCCACTGCTTATTCAACAAGGGGA
AATAGAATAGAAATTTCTTACGAAAATTTACAGGTCACAAATATTACTAGAAAGGGAAGCATGTTACCA
GTGAGCTGTAAGTTACCGAATATGGGTACTTCCCAGAGTTACCTTTCCTCATCTATGCCAGCTGGTTGT
GTTTTGCCAGTAGGTGGTAATTTTGAGATCTTGTTACATTACTATCTTCTCAATTATGCCAAAAAATGC
CATCAATCAGAAGAAACCATGGTTAGTATGATAATAGCTAATGCACTTTTAGGCATTCCCAAAGTCCTT
TATAAATCTAAAACAGGAAAGTACAGCTTTCCACATACATATATAAGAGCTGTCCATGCACTGCAAACC
AATCAACCCTTGGTAAGCAGTCAGACAGGTTTGGAATCAGTAATGGGTAAATACCAGCTACTAACTTCA
GTTCTTCAGTGTTTGACAAAAATATTAACCATTGACATGGTAATCACTGTTAAGAGACACCCTCAGAAA
GTTCACAATCAAGATTCAGAAGATGAACTATAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_024685
Insert Size 2172 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024685.3
RefSeq Size 3583 bp
RefSeq ORF 2172 bp
Locus ID 79738
UniProt ID Q8TAM1
Cytogenetics 12q21.2
MW 80.8 kDa
Summary This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by progressive retinal degeneration, obesity, polydactyly, renal malformation and cognitive disability. The proteins encoded by BBS gene family members are structurally diverse and the similar phenotypes exhibited by mutations in BBS gene family members is likely due to their shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene is likely not a ciliary protein but rather has distant sequence homology to type II chaperonins. As a molecular chaperone, this protein may affect the folding or stability of other ciliary or basal body proteins. Inhibition of this protein's expression impairs ciliogenesis in preadipocytes. Mutations in this gene cause Bardet-Biedl syndrome type 10. provided by RefSeq, Jan 2010
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Documents
Resources
"NM_024685" in other vectors (5)
SKU Description Size Price
RC205501 BBS10 (Myc-DDK-tagged)-Human Bardet-Biedl syndrome 10 (BBS10) 10 ug
$662.00
RC205501L3 Lenti ORF clone of Human Bardet-Biedl syndrome 10 (BBS10), Myc-DDK-tagged 10 ug
$962.00
RC205501L4 Lenti ORF clone of Human Bardet-Biedl syndrome 10 (BBS10), mGFP tagged 10 ug
$962.00
RG205501 BBS10 (tGFP-tagged) - Human Bardet-Biedl syndrome 10 (BBS10) 10 ug
$789.00 $862.00
SC108312 BBS10 (untagged)-Human Bardet-Biedl syndrome 10 (BBS10) 10 ug
$150.00

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