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PHACTR4 (NM_023923) Human Untagged Clone

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SKU
SC318195
PHACTR4 (untagged)-Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 2
  $718.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHACTR4
Synonyms PPP1R124
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318195 representing NM_023923.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGACAAGCTGATGTCTCCAGACCGGTAAATCCAGATGCAGTTGAGGAAGCAGACCAGCCCACTACA
GAGCCAGGCATGGTCCTGGACAGTGTGGAAGCAGGAGACACAACACCTCCTACCAAAAGGAAGAGCAAG
TTCTCAGGCTTTGGCAAGATCTTCAAGCCCTGGAAATGGAGGAAAAAAAAAAGTAGTGATAAATTTAAA
GAGACTTCAGAAGTTTTAGAACGGAAAATATCTATGCGAAAGCCAAGAGAAGAGCTGGTTAAAAGAGGG
GTTCTGTTGGAAGACCCTGAGCAAGGTGGTGAGGATCCAGGAAAGCCAAGCGATGCCATGTTAAAGAAT
GGCCATACCACCCCCATAGGGAATGCCAGATCATCTAGTCCAGTCCAAGTAGAGGAAGAGCCAGTAAGA
TTAGCAAGTCTTAGGAAAGCTATTCCAGAAGAGGACCTAAAGAAACGACTAGGCTCAACTGGAAGCCAG
CCTAATTCTGAAGCAGAGTCTGTTCCTGAGAATGTACCCAAACCACCTTTACTTCCTCCCAAAAGACCC
TTGTCCTCTTCTCATGAAGCAAGTGAAGGGCAAGCAAAGGATGCCACTTCCTCTGGCGGCACGGCAAGG
TTCATCATCTCCACCTCCATCACCACAGCACCCGCTGCCACCACTGCTGCCACAAGCCTTGCAAAGACT
GTTAATCTCTCTGTCACCCCTTCCCCAGCACCCAGGACTCTGCCTGCTGCTCCTGCCAGCACTAACACT
ACTGCTACCCCAAGCCTCACTCATATGGTCCCTGCCAAGCAGCCCCCTATCCCTCCCCCTAAACCAGCT
CACAGAAATAGCAACCCTGTCATTGCTGAACTGTCCCAAGCAATAAACAGTGGTACATTGTTATCAAAA
CCGTCCCCACCCTTACCACCTAAGAGAGGCATTCCATCAACCTCAGTACCCACCTTGGAGTCTGCTGCT
GCCATCACCACAAAAACACCAAGTGATGAAAGAGAGAAGAGCACGTGTTCTATGGGCTCGGAACTACTA
CCAATGATCTCACCTCGCTCTCCGTCCCCCCCACTGCCTACTCATATACCTCCAGAGCCTCCACGCACC
CCTCCATTCCCTGCTAAGACTTTTCAAGTTGTGCCAGAAATTGAGTTTCCACCATCCTTAGATCTACAC
CAGGAGATTCCCCAGCAGGAAGATCAGAAAAAGGAAGTCCCCAAGAGGATACTGGACCAGAACTTTGGG
GAGCCCCATATACCCTCTAGGCTGCCTCCACTCCCACTGCATATTCGAATCCAGCAGGCCCTCACCAGC
CCACTTCCCATGACTCCTATTCTGGAGGGTTCTCACAGAGCTCATTCGTTGCTTTTTGAAAACAGTGAC
AGCTTTTCTGAGGACAGCAGTACGCTGGGTCGGACCAGGTCTCTTCCCATCACTATTGAAATGCTAAAA
GTTCCAGACGATGAAGAAGAAGAGGAGCAAACCTGTCCATCCACATTCAGTGAAGAAATGACACCTACC
TCAGTCATTCCTAAATTACCACAGTGTCTACGGGAGGAAGAAGAGAAGGAGAGCGACTCTGATTCAGAA
GGTCCCATTCAGTACCGAGATGAAGAAGATGAAGATGAAAGCTATCAGAGTGCTCTCGCCAACAAAGTG
AAGAGGAAAGACACACTGGCAATGAAGTTGAACCACAGACCCAGTGAACCAGAGTTGAACCTGAATTCT
TGGCCTTGTAAAAGCAAGGAGGAGTGGAATGAAATACGGCACCAGATTGGAAACACACTGATCCGGCGA
CTGAGTCAAAGACCAACACCAGAAGAACTAGAACAACGCAATATATTGCAACCTAAAAATGAAGCTGAT
CGTCAGGCAGAAAAACGAGAAATTAAACGTCGGCTCACTAGAAAGCTCAGTCAAAGGCCAACTGTCGCT
GAACTCCTTGCCAGGAAGATTCTGAGGTTTAATGAATATGTAGAGGTAACAGATGCTCAAGATTATGAC
CGGCGAGCCGACAAACCTTGGACCAAACTGACCCCTGCTGACAAGGCTGCCATAAGAAAAGAATTAAAT
GAATTTAAAAGCTCCGAGATGGAGGTTCATGAAGAGAGCAAACATTTTACACGCTACCATCGTCCATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_023923
Insert Size 2139 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023923.3
RefSeq Size 6208 bp
RefSeq ORF 2139 bp
Locus ID 65979
UniProt ID Q8IZ21
Cytogenetics 1p35.3
Domains RPEL
MW 79.1 kDa
Summary This gene encodes a member of the phosphatase and actin regulator (PHACTR) family. Other PHACTR family members have been shown to inhibit protein phosphatase 1 (PP1) activity, and the homolog of this gene in the mouse has been shown to interact with actin and PP1. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) contains a distinct 5' UTR and initiates translation at an alternative AUG, compared to variant 1. The resulting isoform (2) has a distinct and longer N-terminus when compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_023923" in other vectors (5)
SKU Description Size Price
RC204311 PHACTR4 (Myc-DDK-tagged)-Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 2 10 ug
$652.00
RC204311L3 Lenti ORF clone of Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 2, Myc-DDK-tagged 10 ug
$952.00
RC204311L4 Lenti ORF clone of Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 2, mGFP tagged 10 ug
$952.00
RG204311 PHACTR4 (tGFP-tagged) - Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 2 10 ug
$789.00 $852.00
SC112339 PHACTR4 (untagged)-Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 2 10 ug
$629.00

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