TMPRSS3 (NM_032405) Human Untagged Clone

SKU SC316502

TMPRSS3 (untagged)-Human transmembrane protease, serine 3 (TMPRSS3), transcript variant D

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMPRSS3
• Synonyms: DFNB8; DFNB10; ECHOS1; TADG12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032405 edited; GGAAAGGGCTGTGTTTATGGGAAGCCAGTAACACTGTGGCCTACTATCTCTTCCGTGGTG; CCATCTACATTTTTGGGACTCGGGAATTATGAGGTAGAGGTGGAGGCGGAGCCGGATGTC; AGAGGTCCTGAAATAGTCACCATGGGGGAAAATGATCCGCCTGCTGTTGAAGCCCCCTTC; TCATTCCGATCGCTTTTTGGCCTTGATGATTTGAAAATAAGTCCTGTTGCACCAGATGCA; GATGCTGTTGCTGCACAGATCCTGTCACTGCTGCCATTGAAGTTTTTTCCAATCATCGTC; ATTGGGATCATTGCATTGATATTAGCACTGGCCATTGGTCTGGGCATCCACTTCGACTGC; TCAGGGAAGTACAGATGTCGCTCATCCTTTAAGTGTATCGAGCTGATAGCTCGATGTGAC; GGAGTCTCGGATTGCAAAGACGGGGAGGACGAGTACCGCTGTGTCCGGGTGGGTGGTCAG; AATGCCGTGCTCCAGGTGTTCACAGCTGCTTCGTGGAAGACCATGTGCTCCGATGACTGG; AAGGGTCACTACACAAATGTTGCCTGTGCCCAACTGGGTTTCCCAAGCTATGTGAGTTCA; GATAACCTCAGAGTGAGCTCGCTGGAGGGGCAGTTCCGGGAGGAGTTTGTGTCCATCGAT; CACCTCTTGCCAGATGACAAGGTGACTGCATTACACCACTCAGTATATGTGAGGGAGGGA; TGTGCCTCTGGCCACGTGGTTACCTTGCAGTGCACAGCCTGTGGTCATAGAAGGGGCTAC; AGCTCACGCATCGTGGGTGGAAACATGTCCTTGCTCTCGCAGTGGCCCTGGCAGGCCAGC; CTTCAGTTCCAGGGCTACCACCTGTGCGGGGGCTCTGTCATCACGCCCCTGTGGATCATC; ACTGCTGCACACTGTGTTTATGACTTGTACCTCCCCAAGTCATGGACCATCCAGGTGGGT; CTAGTTTCCCTGTTGGACAATCCAGCCCCATCCCACTTGGTGGAGAAGATTGTCTACCAC; AGCAAGTACAAGCCAAAGAGGCTGGGCAATGACATCGCCCTTATGAAGCTGGCCGGGCCA; CTCACGTTCAATGGTACATCTGGGTCTCTATGTGGTTCTGCAGCTCTTCCTTTGTTTCAA; GAGGATTTGCAATTGCTCATTGAAGCATTCTTATGATCTAGATT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032405 unedited; GGGCTCCTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGGAAAGGGCT; GTGTTTATGGGAAGCCAGTAACACTGTGGCCTACTATCTCTTCCGTGGTGCCATCTACAT; TTTTGGGACTCGGGAATTATGAGGTAGAGGTGGAGGCGGAGCCGGATGTCAGAGGTCCTG; AAATAGTCACCATGGGGGAAAATGATCCGCCTGCTGTTGAAGCCCCCTTCTCATTCCGAT; CGCTTTTTGGCCTTGATGATTTGAAAATAAGTCCTGTTGCACCAGATGCAGATGCTGTTG; CTGCACAGATCCTGTCACTGCTGCCATTGAAGTTTTTTCCAATCATCGTCATTGGGATCA; TTGCATTGATATTAGCACTGGCCATTGGTCTGGGCATCCACTTCGACTGCTCAGGGAAGT; ACAGATGTCGCTCATCCTTTAAGTGTATCGAGCTGATAGCTCGATGTGACGGAGTCTCGG; ATTGCAAAGACGGGGAGGACGAGTACCGCTGTGTCCGGGTGGGTGGTCAGAATGCCGTGC; TCCAGGTGTTCACAGCTGCTTCGTGGAAGACCATGTGCTCCGATGACTGGAAGGGTCACT; ACACAAATGTTGCCTGTGCCCAACTGGGTTTCCCAAGCTATGTGAGTTCAGATAACCTCA; GAGTGAGCTCGCTGGAGGGGCAGTTCCGGGAGGAGTTTGTGTCCATCGATCACCTCTTGC; CAGATGACAAGGTGACTGCATTACACCACTCAGTATATGTGAGGGAGGGATGTGCCTCTG; GCCACGTGGTTACCTTGCAGTGCACAGCCTGTGGTCATAGAAGGGCTACAGCTCACGCAT; CGTGGGTGGAAACATGTCCTTGCTCTCGCAGTGGCCCTGGCAGGCCAGCCTTCAGTTTCA; GGGCTACCACCTGGTGCGGGGGGCTTCTGTCATCACGCCCCTGTGTGGATTCATCACCTG; CTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032405 unedited; GGTTCGCGAGATGCTGAGATGCACTTCAGGCCGGAAGCACTGGGGAAGGGTCACAGGGAT; GCCCCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATCATAAGAAT; GCTTCAATGAGCAATTGCAAATCCTCTTGAAACAAAGGAAGAGCTGCAGAACCACATAGA; GACCCAGATGTACCATTGAACGTGAGTGGCCCGGCCAGCTTCATAAGGGCGATGTCATTG; CCCAGCCTCTTTGGCTTGTACTTGCTGTGGTAGACAATCTTCTCCACCAAGTGGGATGGG; GCTGGATTGTCCAACAGGGAAACTAGACCCACCTGGATGGTCCATGACTTGGGGAGGTAC; AAGTCATAAACACAGTGTGCAGCAGTGATGATCCACAGGGGCGTGATGACAGAGCCCCCG; CACAGGTGGTAGCCCTGGAACTGAAGGCTGGCCTGCCAGGGCCACTGCGAGAGCAAGGAC; ATGTTTCCACCCACGATGCGTGAGCTGTAGCCCCTTCTATGACCACAGGCTGTGCACTGC; AAGGTAACCACGTGGCCAGAGGCACATCCCTCCCTCACATATACTGAGTGGTGTAATGCA; GTCACCTTGTCATCTGGCAAGAGGTGATCGATGGACACAAACTCCTCCCGGAACTGCCCC; TCCAGCGAGCTCACTCTGAGGTTATCTGAACTCACATAGCTTGGGAAACCCAGTTGGGCA; CAGGCAACATTTGTGTAGTGACCCTTCCAGTCATCGGAGCACATGGTCTTCCACGAAGCA; GCTGTGAACACCTGGAGCACGGCATTCTGACCACCCCACCCGGACACAGCGGTACTCGTC; CTCCCGTCTTTGCAATCCGAGACTCCGTCACCATCGAGCTATCAGCTCGATACACTTAAA; GGATGAGCGACATCTGTACTTCCCTGAGCAGTCGA;
• Restriction Sites: Please inquire
• ACCN: NM_032405
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032405.1, NP_115781.1
• RefSeq Size: 1359 bp
• RefSeq ORF: 1035 bp
• Locus ID: 64699
• UniProt ID: P57727
• Cytogenetics: 21q22.3
• Domains: ldl_recept_a, SR, Tryp_SPc
• Protein Families: Druggable Genome, Protease, Transmembrane
• Summary: This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a serine protease domain, a transmembrane domain, an LDL receptor-like domain, and a scavenger receptor cysteine-rich domain. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified by its association with both congenital and childhood onset autosomal recessive deafness. This gene is expressed in fetal cochlea and many other tissues, and is thought to be involved in the development and maintenance of the inner ear or the contents of the perilymph and endolymph. This gene was also identified as a tumor-associated gene that is overexpressed in ovarian tumors. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012]; Transcript Variant: This variant (D) lacks several 3' exons, but includes an alternate 3' exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant A. The encoded isoform (3) has a distinct and shorter C-terminus, compared to isoform 1.