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USP35 (NM_020798) Human Untagged Clone

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SKU
SC316437
USP35 (untagged)-Human ubiquitin specific peptidase 35 (USP35)
  $976.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol USP35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC316437 representing NM_020798.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGTCAGCGTGAAGCAGGGGCTGGTTCGG
CGCGTGCTGGAGGCGGCGCGGCAGCCGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCG
CGCCTCTACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCTGCACGTGGCCGGC
CGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCGCGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGC
GCCGGCCCCCCGGGCCCCCGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCCT
GCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCGTGTGCGAGCGCCCGGGCCCC
GCGGCCTGCGCGCAGGTGGCACGGCTGCTGGCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGC
CTGCTCTTCTGCCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCGAGGAGGGC
GCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTCCTGGCGCAGCTGTGGCGCGCACAGCCC
GCCGCCATCCTGCCCTGCCTCAAAGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCT
AGCGCCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTTGTCCGGAACCTCAGC
AATGATGACAGTGTGACAGACTCGCAGATGCTGACTGCCATTAGCAGGATGATTGACTGGGTGTCCTGG
CCCCTGGGGAAGAATATTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAGTTC
AGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTCTAAGCTGCTGTACCCCATCGTC
CGGGGAGCTGCCTTGTCTGTGCTCAAGTACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCAC
CTGCTCCTCCCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTCGGGGACCAGC
TGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGTTCCGGTTCCCGGGCTTCCCGGATCTGTAT
GAGCCTGTCATGGAGGCCATCAAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGG
CAGGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGCTCATGGCCAAGTCAGAC
ACGGGCAAGATTGGTCTCATCAACCTGGGCAACACATGCTATGTCAACAGCATCCTTCAGGCCTTATTC
ATGGCGTCTGACTTCAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGACCAAG
CTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCCATTTCCCCAGAGAACTTCCTCTCC
GCATCCTGGACGCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTG
GATCGGCTGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAGTCCAGCTCGCCC
TCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTCTGTGGAAAAAATGTTTGGAGGCAAGATAGTG
ACTCGGATCTGCTGTCTCTGCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC
GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGATGCGCCCCACAGAAGACATC
ACAGCCCGGGAGTTGCCCCCACCAACCAGTGCACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGG
AAACACTGCATCACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAAGGAAGCT
GGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAGAAGGGAAGGAGGAGAGAACGGAGAAG
GAAGAAGTGGGGGAGGAGGAGGAAAGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAA
GAGGAAGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGGAAGAAGACAGCCTG
GGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCCGGGGAGCGGACATGTGGCTCTGAGGGCTCCCGC
TCCGTCCTGGACCTGGTTAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACTGC
GAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGCCAAGGGCCGTGCTACCTCATC
CTCACACTGCTGCGCTTCTCTTTCGACCTGCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCC
ATCCCCCTGCTGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGCAGTGTGGTG
GTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTGCTATGCCCGTGAGGGCGCTGCCCGCCCT
GCCGCTTCTCTGGGAACTGCCGATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGG
GTGTCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAAGGACACAGCCTATGTG
CTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCGAGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAG
CCCACCCTGCACAAGGACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCAGGAG
AAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACATCTCCGCACTGGGGGAGGGGCTTT
GATGAAGACAAGGATGAGGATGAAGGCTCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGAC
TTCCACAGACTGGTCTTCTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_020798
Insert Size 3057 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020798.2
RefSeq Size 4311 bp
RefSeq ORF 3057 bp
Locus ID 57558
UniProt ID Q9P2H5
Cytogenetics 11q14.1
Protein Families Druggable Genome, Protease
MW 113.4 kDa
Summary This gene encodes a member of the peptidase C19 family of ubiquitin-specific proteases. These deubiquitinating enzymes (DUBs) catalyze the removal of ubiquitin proteins from other proteins. The encoded protein associates with polarized mitochondria and has been shown to inhibit NF-kappa B activation and delay PARK2-mediated degradation of mitochondria. Expression of this gene is upregulated by the let-7a microRNA and reduced expression has been observed in human tumor tissues. provided by RefSeq, Jul 2017
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"NM_020798" in other vectors (6)
SKU Description Size Price
RC219955 USP35 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 35 (USP35) 10 ug
$886.00
RC219955L1 Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), Myc-DDK-tagged 10 ug
$1,186.00
RC219955L2 Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), mGFP tagged 10 ug
$1,186.00
RC219955L3 Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), Myc-DDK-tagged 10 ug
$1,186.00
RC219955L4 Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), mGFP tagged 10 ug
$1,186.00
RG219955 USP35 (tGFP-tagged) - Human ubiquitin specific peptidase 35 (USP35) 10 ug
$1,086.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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