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CDC14B (NM_001077181) Human Untagged Clone

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SKU
SC315434
CDC14B (untagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 3
  $503.00
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Other products for "CDC14B or NM_001077181"

Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CDC14B
Synonyms Cdc14B1; Cdc14B2; CDC14B3; hCDC14B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_001077181
Insert Size 1386 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001077181.1
RefSeq Size 5131 bp
RefSeq ORF 1386 bp
Locus ID 8555
UniProt ID O60729
Cytogenetics 9q22.32-q22.33
Protein Families Druggable Genome, Phosphatase
Protein Pathways Cell cycle
MW 52.3 kDa
Summary The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (3) has an alternate 5' exon, compared to variant 2, resulting in a protein (isoform 3) that has an alternate N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_001077181" in other vectors (4)
SKU Description Size Price
RC221171 CDC14B (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 3 10 ug
$457.00
RC221171L3 Lenti ORF clone of Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC221171L4 Lenti ORF clone of Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 3, mGFP tagged 10 ug
$757.00
RG221171 CDC14B (tGFP-tagged) - Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 3 10 ug
$657.00

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