PHLPP2 (NM_015020) Human Untagged Clone

SKU SC315357

PHLPP2 (untagged)-Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PHLPP2
• Synonyms: PHLPPL; PPM3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315357 representing NM_015020.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAAACGCAATGGGAGCAGAAATTGTTTGAATAGGAGAAGTAGGTTTGGTTCTCGAGAAAGAGACTGG; CTAAGAGAAGATGTAAAGAGAGGCTGTGTTTACCTTTATGGAGCAGACACTACCACTGCCACTACAACC; ACCACCACCTCCTCTTCCTCTTCCTCCTCCTCCTCTTCCTCTGACTTACATCTCGTCCTTTGCACTGTA; GAGACACCAGCATCAGAAATATGTGCTGGAGAGGGAAGAGAAAGTCTTTATTTACAGCTTCATGGAGAC; CTGGTCAGGAGACTGGAACCTACTGAACGACCTCTTCAGATCGTTTATGATTACTTATCCAGGCTGGGA; TTTGATGATCCTGTGCGCATACAGGAGGAGGCTACAAATCCTGACCTCGGCTGTATGATTCGATTTTAT; GGTGAAAAACCATGCCACATGGATCGTTTGGATCGAATCCTATTGTCTGGCATCTATAATGTACGCAAG; GGAAAGACCCAGCTGCATAAGTGGGCTGAGCGCCTAGTTGTCCTCTGTGGTACCTGCCTTATCGTTTCC; TCAGTGAAGGATTGTCAAACTGGAAAGATGCACATTTTGCCTCTGGTTGGTGGAAAGATAGAAGAAGTG; AAGCGACGGCAATACTCCCTTGCTTTCAGCTCAGCAGGAGCCCAAGCTCAGACCTATCATGTCAGCTTC; GAGACTTTGGCCGAGTACCAGCGATGGCAACGGCAAGCATCCAAGGTGGTGTCCCAGCGAATCAGTACC; GTGGATCTCTCGTGTTACAGCCTCGAGGAGGTTCCTGAGCATCTCTTCTATAGTCAAGATATTACCTAC; CTCAACTTGCGACACAACTTCATGCAGTTAGAAAGACCCGGAGGCCTCGATACACTCTACAAATTTTCT; CAACTGAAGGGCCTGAACTTGTCCCATAATAAACTTGGGTTGTTTCCTATATTGTTATGCGAGATCTCT; ACCCTGACTGAGCTCAACCTTTCCTGTAATGGATTTCATGACCTACCAAGTCAAATTGGCAATCTGCTA; AATCTTCAAACCCTCTGTCTTGATGGCAACTTTCTGACTACTTTACCTGAAGAATTGGGAAATCTACAA; CAGCTTTCCTCCTTGGGAATTTCCTTCAACAACTTTAGTCAAATTCCTGAGGTTTATGAGAAACTCACT; ATGTTAGATAGAGTGGTTATGGCAGGAAATTGCCTGGAAGTCCTGAACTTAGGGGTGCTGAATAGGATG; AACCATATCAAGCATGTGGATTTAAGGATGAACCATTTGAAAACCATGGTTATTGAAAATCTGGAGGGA; AATAAACACATCACCCACGTGGATCTGCGGGACAACCGACTGACTGACTTGGATCTTAGCTCCTTATGC; AGCTTGGAACAGCTGCACTGTGGGCGGAATCAGCTGAGGGAGCTAACACTCAGTGGCTTTTCCCTTCGG; ACCCTCTATGCCAGTTCCAACAGGCTGACAGCAGTGAACGTCTATCCAGTACCCAGCCTGCTCACTTTC; TTGGATCTCTCCCGAAACCTGCTAGAGTGTGTCCCTGACTGGGCCTGTGAAGCAAAGAAGATAGAAGTA; TTAGATGTGAGCTATAATCTTCTCACAGAGGTTCCCGTGAGAATTCTGAGTAGCTTGAGTCTTAGAAAA; CTGATGCTGGGACACAATCATGTGCAAAACCTTCCAACACTGGTAGAGCACATCCCCCTCGAGGTGCTG; GATCTTCAGCATAATGCACTCACGAGGCTGCCAGACACCCTCTTCTCCAAGGCCTTAAATCTCAGATAC; TTGAATGCATCTGCAAATAGTCTGGAGTCTTTACCATCCGCCTGCACTGGAGAGGAGAGTTTGAGTATG; CTGCAGCTGCTTTATCTGACCAACAATCTCCTGACGGATCAGTGCATACCTGTCCTGGTAGGGCACCTG; CACCTGCGAATCTTGCACCTTGCAAACAATCAGTTACAGACCTTTCCTGCAAGCAAACTAAATAAATTG; GAGCAATTGGAGGAACTGAACCTAAGTGGCAACAAGCTTAAAACCATTCCCACAACCATAGCAAACTGT; AAAAGGCTGCACACCCTTGTTGCACACTCCAACAACATCAGCATTTTCCCAGAAATACTGCAGTTGCCT; CAGATCCAGTTTGTAGACCTAAGTTGCAACGACTTGACAGAAATCCTGATTCCAGAGGCTTTGCCTGCT; ACATTACAAGACCTTGACCTGACTGGAAATACAAATCTGGTTCTGGAACACAAGACACTGGACATATTT; AGCCATATCACAACCCTGAAAATTGATCAGAAACCTTTGCCAACCACAGATTCTACAGTTACGTCAACC; TTCTGGAGCCATGGACTGGCTGAGATGGCAGGGCAGAGAAATAAGCTGTGTGTCTCAGCACTTGCTATG; GATAGCTTTGCAGAGGGGGTGGGAGCTGTGTATGGCATGTTTGATGGAGACCGAAATGAGGAGCTCCCG; CGCCTGCTGCAGTGTACGATGGCAGATGTGCTTTTAGAAGAGGTACAGCAGTCAACTAATGACACAGTT; TTCATGGCTAACACCTTCTTGGTATCTCACAGGAAATTAGGAATGGCTGGCCAGAAGTTGGGCTCCTCC; GCTCTCCTGTGCTACATCCGCCCTGACACTGCCGATCCAGCAAGTAGCTTTAGCTTGACTGTAGCCAAT; GTTGGCACGTGCCAAGCAGTCCTGTGCCGAGGTGGGAAGCCAGTGCCCCTCTCTAAAGTCTTCAGCCTG; GAGCAGGACCCAGAGGAGGCTCAAAGGGTGAAGGACCAAAAAGCCATCATCACAGAGGACAACAAAGTG; AATGGGGTAACCTGCTGTACCCGGATGCTGGGCTGTACATACCTCTACCCTTGGATCCTCCCCAAGCCC; CACATATCTTCCACTCCGCTGACCATTCAAGATGAGTTGCTGATTCTGGGAAACAAAGCATTGTGGGAA; CACTTGTCCTACACAGAAGCTGTCAATGCTGTACGTCACGTACAAGACCCATTAGCAGCTGCTAAGAAG; CTGTGCACATTAGCGCAGAGCTATGGCTGTCAGGACAATGTAGGGGCGATGGTAGTTTATTTGAATATT; GGTGAGGAAGGCTGCACTTGTGAAATGAATGGGCTCACCCTCCCAGGTCCTGTGGGATTTGCTTCAACC; ACCACTATCAAGGATGCCCCTAAGCCAGCCACTCCATCCTCTAGCAGTGGGATTGCCTCTGAGTTCAGC; AGTGAGATGTCCACCTCAGAGGTGAGCAGTGAAGTGGGGTCCACTGCTTCTGATGAGCATAATGCTGGG; GGCCTGGACACTGCCTTGCTTCCGAGGCCAGAGCGGCGCTGCAGCCTCCACCCAACACCCACCTCTGGG; CTGTTTCAGCGCCAGCCTTCTTCTGCTACCTTCTCCAGTAACCAGTCTGACAACGGCCTGGACAGTGAT; GATGACCAGCCCGTTGAGGGGGTCATAACCAATGGCAGCAAGGTAGAGGTGGAAGTAGACATCCACTGC; TGCAGGGGGAGGGATCTGGAGAACTCACCCCCTCTCATAGAGAGTTCTCCTACCCTGTGTTCTGAGGAA; CATGCTAGAGGGTCGTGTTTTGGGATCCGAAGACAGAACAGTGTGAATAGTGGCATGCTCCTGCCAATG; AGCAAGGACAGGATGGAGTTACAGAAGTCTCCCTCCACCTCCTGCCTCTATGGGAAGAAACTCTCCAAT; GGCTCTATTGTGCCCCTAGAGGACAGCCTGAACCTCATTGAAGTGGCCACAGAAGTGCCCAAGAGGAAA; ACTGGCTATTTTGCTGCCCCCACTCAGATGGAACCAGAGGACCAGTTTGTTGTGCCTCATGACCTGGAA; GAAGAAGTGAAGGAACAAATGAAACAGCACCAGGACAGCCGGCTCGAGCCTGAGCCCCATGAAGAGGAT; CGGACCGAGCCCCCGGAGGAGTTCGACACAGCACTATGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_015020
• Insert Size: 3972 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015020.3
• RefSeq Size: 8317 bp
• RefSeq ORF: 3972 bp
• Locus ID: 23035
• UniProt ID: Q6ZVD8
• Cytogenetics: 16q22.2
• MW: 146.8 kDa
• Summary: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.