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FBH1 (NM_032807) Human Untagged Clone

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SKU
SC315174
FBXO18 (untagged)-Human F-box protein, helicase, 18 (FBXO18), transcript variant 1
  $953.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FBH1
Synonyms Fbx18; FBXO18; hFBH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC315174 representing NM_032807.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGCTACGAGGTGACTTCAGGCTGCCATTGGACCTGTCAAGTGCCTGAGTCATGTGATAATGGGCTA
CATTGCGCAGGGCCCCTGGGCCATCTCCACAGGAGATGCCAGAGGACGAGTGCCCACTTGCTGGTCTTC
ACAGAGCACGCTGAAATGAGACGGTTTAAGCGGAAGCATCTTACTGCCATTGACTGCCAGCATTTGGCT
CGGAGTCACTTGGCTGTGACCCAGCCCTTCGGTCAAAGATGGACAAACAGAGATCCGAACCATGGTCTC
TATCCTAAACCGAGAACAAAAAGAGGGAGTAGGGGTCAGGGAAGTCAAAGATGCATCCCTGAGTTCTTC
CTAGCAGGCAAGCAGCCGTGCACCAATGACATGGCCAAAAGCAATTCTGTTGGCCAGGACAGCTGTCAG
GACTCTGAGGGTGACATGATCTTTCCTGCAGAGAGCAGCTGTGCACTGCCTCAGGAAGGCAGTGCAGGG
CCGGGCTCACCAGGGTCTGCCCCGCCCTCCAGGAAGCGGTCTTGGTCCTCTGAGGAAGAGAGTAACCAG
GCTACCGGGACCAGCCGGTGGGATGGAGTTTCTAAGAAAGCTCCACGGCACCATTTGTCTGTGCCATGC
ACAAGGCCTAGGGAGGCCAGGCAAGAAGCAGAGGACAGTACGTCTCGGCTCTCTGCGGAGTCTGGTGAA
ACCGACCAAGATGCTGGGGACGTGGGTCCTGATCCCATTCCTGACTCATACTATGGGCTTCTTGGGACC
TTGCCCTGCCAGGAAGCACTGAGCCACATTTGCAGCCTGCCTAGTGAGGTCCTGAGGCACGTGTTTGCC
TTCCTCCCGGTGGAAGACCTCTATTGGAACCTGAGCTTGGTGTGCCACTTGTGGAGGGAGATCATCAGT
GACCCGCTGTTCATTCCTTGGAAGAAGCTGTACCATCGATACCTGATGAATGAAGAGCAAGCTGTCAGC
AAAGTGGACGGCATCCTGTCTAACTGTGGCATAGAAAAGGAGTCAGACCTGTGTGTGCTGAACCTCATA
CGATACACAGCCACCACTAAGTGCTCTCCGAGTGTGGATCCCGAGAGGGTGCTGTGGAGTCTGAGGGAC
CACCCCCTCCTCCCCGAGGCTGAGGCGTGTGTGCGGCAACACCTCCCCGACCTCTACGCTGCTGCCGGG
GGTGTCAACATCTGGGCCCTGGTGGCGGCTGTGGTGCTCCTCTCCAGCAGTGTGAATGACATCCAGCGA
CTGCTCTTCTGCCTCCGGAGACCCAGCTCCACGGTGACCATGCCAGATGTCACCGAGACCCTGTACTGC
ATAGCCGTGCTTCTCTACGCCATGAGGGAGAAGGGGATTAACATCAGCAATAGGATTCACTACAACATT
TTCTATTGCCTATATCTTCAGGAGAATTCCTGCACTCAGGCCACAAAAGTTAAAGAGGAGCCATCTGTC
TGGCCAGGCAAGAAAACCATCCAACTTACACATGAACAACAGCTGATTCTGAATCACAAGATGGAACCT
CTCCAGGTGGTGAAAATTATGGCCTTTGCCGGCACTGGGAAGACCTCAACGCTGGTCAAGTATGCAGAG
AAGTGGTCTCAGAGCAGGTTTCTGTATGTGACATTCAACAAGAGCATCGCAAAGCAGGCCGAACGCGTC
TTCCCCAGCAACGTCATCTGCAAAACCTTCCACTCCATGGCCTACGGGCACATAGGGCGGAAGTACCAG
TCAAAGAAGAAGTTGAATCTCTTCAAGTTAACACCCTTCATGGTCAACTCCGTCCTTGCTGAAGGGAAG
GGTGGATTCATAAGAGCCAAGCTTGTGTGTAAGACTCTAGAAAACTTCTTTGCCTCGGCTGACGAAGAG
CTGACCATTGATCACGTGCCTATTTGGTGTAAGAACAGCCAAGGACAGAGAGTCATGGTTGAGCAGAGT
GAAAAACTGAATGGTGTCCTTGAAGCGAGCCGCCTCTGGGATAACATGCGGAAGCTGGGGGAGTGCACA
GAAGAGGCGCACCAGATGACTCATGACGGCTACTTGAAACTCTGGCAGCTGAGCAAGCCTTCGCTGGCC
TCTTTTGACGCCATCTTTGTGGATGAGGCCCAGGACTGCACACCAGCTATCATGAACATAGTTCTGTCT
CAGCCATGTGGGAAAATCTTTGTAGGGGACCCGCACCAGCAGATCTATACCTTCCGGGGTGCGGTCAAC
GCCCTGTTCACAGTGCCCCACACCCACGTCTTCTATCTCACGCAGAGTTTTCGGTTTGGTGTGGAAATA
GCTTATGTGGGAGCTACTATCTTGGATGTTTGCAAGAGAGTCAGGAAAAAGACTTTGGTTGGAGGAAAC
CATCAGAGTGGCATTAGAGGTGACGCAAAGGGGCAAGTGGCCTTGTTGTCCCGGACCAACGCCAACGTG
TTTGATGAGGCCGTACGGGTGACGGAAGGGGAATTCCCTTCAAGGATACATTTGATTGGGGGGATTAAA
TCATTTGGATTGGACAGAATCATTGATATTTGGATCCTTCTTCAGCCAGAGGAAGAACGGAGGAAACAA
AACCTCGTCATTAAAGACAAATTTATCAGAAGATGGGTGCACAAAGAAGGCTTTAGTGGCTTCAAGAGG
TATGTGACCGCTGCCGAGGACAAGGAGCTTGAAGCCAAGATCGCAGTTGTTGAAAAGTATAACATCAGG
ATTCCAGAGCTGGTGCAAAGGATAGAAAAATGCCATATAGAAGATTTGGACTTTGCAGAGTACATTCTG
GGCACTGTGCACAAAGCCAAAGGCCTGGAGTTTGACACTGTGCATGTTTTGGATGATTTTGTGAAAGTG
CCTTGTGCCCGGCATAACCTGCCCCAGCTTCCGCACTTCAGAGTTGAGTCATTTTCTGAGGATGAATGG
AATTTACTGTATGTTGCAGTAACTCGAGCCAAGAAGCGTCTCATCATGACCAAATCATTGGAAAACATT
TTGACTTTGGCTGGGGAGTACTTCTTGCAAGCAGAGCTGACAAGCAACGTCTTAAAAACAGGCGTGGTG
CGCTGCTGCGTGGGACAGTGCAACAATGCCATCCCTGTTGACACCGTCCTTACCATGAAGAAGCTGCCC
ATCACCTATAGCAACAGGAAGGAAAACAAGGGGGGCTACCTCTGCCACTCCTGTGCGGAGCAGCGCATC
GGGCCCCTGGCGTTCCTGACAGCCTCCCCGGAGCAGGTGCGCGCCATGGAGCGCACTGTGGAGAACATC
GTACTGCCCCGGCATGAGGCCCTGCTCTTCCTCGTCTTCTGA
AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_032807
Insert Size 3285 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032807.3
RefSeq Size 3718 bp
RefSeq ORF 3285 bp
Locus ID 84893
UniProt ID Q8NFZ0
Cytogenetics 10p15.1
Domains F-box
Protein Families Druggable Genome
MW 123.4 kDa
Summary This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_032807" in other vectors (4)
SKU Description Size Price
RC214006 FBXO18 (Myc-DDK-tagged)-Human F-box protein, helicase, 18 (FBXO18), transcript variant 1 10 ug
$952.00
RC214006L3 Lenti ORF clone of Human F-box protein, helicase, 18 (FBXO18), transcript variant 1, Myc-DDK-tagged 10 ug
$1,252.00
RC214006L4 Lenti ORF clone of Human F-box protein, helicase, 18 (FBXO18), transcript variant 1, mGFP tagged 10 ug
$1,252.00
RG214006 FBXO18 (tGFP-tagged) - Human F-box protein, helicase, 18 (FBXO18), transcript variant 1 10 ug
$1,152.00

file-alt Citations

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