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ILF3 (NM_153464) Human Untagged Clone

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SKU
SC314442
ILF3 (untagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3
  $696.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ILF3
Synonyms CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF90; NF90a; NF90b; NF90c; NF90ctv; NF110; NF110b; NFAR; NFAR-1; NFAR-2; NFAR2; NFAR90; NFAR110; TCP80; TCP110
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC314442 representing NM_153464.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCGTCCAATGCGAATTTTTGTGAATGATGACCGCCATGTGATGGCAAAGCATTCTTCCGTTTATCCA
ACACAAGAGGAGCTGGAGGCAGTCCAGAACATGGTGTCCCACACGGAGCGGGCGCTCAAAGCTGTGTCC
GACTGGATAGACGAGCAGGAAAAGGGTAGCAGCGAGCAGGCAGAGTCCGATAACATGGATGTGCCCCCA
GAGGACGACAGTAAAGAAGGGGCTGGGGAACAGAAGACGGAGCACATGACCAGAACCCTGCGGGGAGTG
ATGCGGGTGGGCCTGGTGGCAAAGGGCCTCCTACTCAAGGGGGACTTGGATCTGGAGCTGGTGCTGCTG
TGTAAGGAGAAGCCCACAACCGCCCTCCTGGACAAGGTGGCCGACAACCTGGCCATCCAGCTTGCTGCT
GTAACAGAAGACAAGTACGAAATACTGCAATCTGTCGACGATGCTGCGATTGTGATAAAAAACACAAAA
GAGCCTCCATTGTCCCTGACCATCCACCTGACATCCCCTGTTGTCAGAGAAGAAATGGAGAAAGTATTA
GCTGGAGAAACGCTATCAGTCAACGACCCCCCGGACGTTCTGGACAGGCAGAAATGCCTTGCTGCCTTG
GCGTCCCTCCGACACGCCAAGTGGTTCCAGGCCAGAGCCAACGGGCTGAAGTCTTGTGTCATTGTGATC
CGGGTCTTGAGGGACCTGTGCACTCGCGTGCCCACCTGGGGTCCCCTCCGAGGCTGGCCTCTCGAGCTC
CTGTGTGAGAAATCCATTGGCACGGCCAACAGACCGATGGGTGCTGGCGAGGCCCTGCGGAGAGTGCTG
GAGTGCCTGGCGTCGGGCATCGTGATGCCAGATGGTTCTGGCATTTATGACCCTTGTGAAAAAGAAGCC
ACTGATGCTATTGGGCATCTAGACAGACAGCAACGGGAAGATATCACACAGAGTGCGCAGCACGCACTG
CGGCTCGCTGCCTTCGGCCAGCTCCATAAAGTCCTAGGCATGGACCCTCTGCCTTCCAAGATGCCCAAG
AAACCAAAGAATGAAAACCCAGTGGACTACACCGTTCAGATCCCACCAAGCACCACCTATGCCATTACG
CCCATGAAACGCCCAATGGAGGAGGACGGGGAGGAGAAGTCGCCCAGCAAAAAGAAGAAGAAGATTCAG
AAGAAAGAGGAGAAGGCAGAGCCCCCCCAGGCTATGAATGCCCTGATGCGGTTGAACCAGCTGAAGCCA
GGGCTGCAGTACAAGCTGGTGTCCCAGACTGGGCCCGTCCATGCCCCCATCTTTACCATGTCTGTGGAG
GTTGATGGCAATTCATTCGAGGCCTCTGGGCCCTCCAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAG
GTGTTACAGGACATGGGCTTGCCGACGGGTGCTGAAGGCAGGGACTCGAGCAAGGGGGAGGACTCGGCT
GAGGAGACCGAGGCGAAGCCAGCAGTGGTGGCCCCTGCCCCAGTGGTAGAAGCTGTCTCCACCCCTAGT
GCGGCCTTTCCCTCAGATGCCACTGCCGAGCAGGGGCCGATCCTGACAAAGCACGGCAAGAACCCAGTC
ATGGAGCTGAACGAGAAGAGGCGTGGGCTCAAGTACGAGCTCATCTCCGAGACCGGGGGCAGCCACGAC
AAGCGCTTCGTCATGGAGGTCGAAGTGGATGGACAGAAGTTCCAAGGTGCTGGTTCCAACAAAAAGGTG
GCGAAGGCCTACGCTGCTCTTGCTGCCCTAGAAAAGCTTTTCCCTGACACCCCTCTCGCCCTTGATGCC
AACAAAAAGAAGAGAGCCCCAGTACCCGTCAGAGGGGGACCGAAATTTGCTGCTAAGCCACATAACCCT
GGCTTCGGCATGGGAGGCCCCATGCACAACGAAGTGCCCCCACCCCCCAACCTTCGAGGGCGGGGAAGA
GGCGGGAGCATCCGGGGACGAGGGCGCGGGCGAGGATTTGGTGGCGCCAACCATGGAGGCTACATGAAT
GCCGGTGCTGGGTATGGAAGCTATGGGTACGGAGGCAACTCGGCGACAGCAGGCTACACAGGTTTTGTA
TGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_153464
Insert Size 2073 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153464.2
RefSeq Size 3302 bp
RefSeq ORF 2073 bp
Locus ID 3609
UniProt ID Q12906
Cytogenetics 19p13.2
Protein Families Druggable Genome, Transcription Factors
MW 74.6 kDa
Summary This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.provided by RefSeq, Dec 2014
Transcript Variant: This variant (3) differs in the 3' UTR and coding region compared to variant 4. The resulting isoform (c) has a distinct C-terminus compared to isoform d. This isoform is also called DRBP76 delta and ILF3-C.
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Documents
Resources
"NM_153464" in other vectors (4)
SKU Description Size Price
RC219617 ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 10 ug
$632.00
RC219617L3 Lenti-ORF clone of ILF3 (Myc-DDK-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 10 ug
$932.00
RC219617L4 Lenti-ORF clone of ILF3 (mGFP-tagged)-Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 10 ug
$932.00
RG219617 ILF3 (tGFP-tagged) - Human interleukin enhancer binding factor 3, 90kDa (ILF3), transcript variant 3 10 ug
$832.00

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